LOCUS HSU04953 4508 bp mRNA linear HUM 31-OCT-1995
DEFINITION Human isoleucyl-tRNA synthetase mRNA, complete cds.
ACCESSION U04953
VERSION U04953.1
KEYWORDS IRS.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4508)
AUTHORS Nichols,R.C., Raben,N., Boerkoel,C.F. and Plotz,P.H.
TITLE Human isoleucyl-tRNA synthetase: sequence of the cDNA, alternative
mRNA splicing, and the characteristics of an unusually long
C-terminal extension
JOURNAL Gene 155 (2), 299-304 (1995)
PUBMED 7721108
REFERENCE 2 (bases 1 to 4508)
AUTHORS Nichols,R.C.
TITLE Direct Submission
JOURNAL Submitted (07-JAN-1994) Ralph C. Nichols, National Institutes of
Health, Arthritis and Rheumatism Branch, 9000 Rockville Pike,
Bethesda, MD 20892, USA
COMMENT On Jan 27, 1994 this sequence version replaced gi:440798.
FEATURES Location/Qualifiers
source 1..4508
/db_xref="H-InvDB:HIT000217576"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="Hep G2"
/cell_type="hepatocyte"
/tissue_type="liver"
/clone_lib="Hep G2 cDNA library, Stratagene"
intron 79..248
/note="This region is excised in some transcripts and is
therefore probably an intron. This suggests that the ATG
at 256 rather than the one at 245 is the start codon."
CDS 256..4044
/EC_number="6.1.1.5"
/function="ligates isoleucine to isoleucyl-tRNA"
/note="The following aa are consensus sequences: HYGH(aa
55-58), WTISR(aa 455-459), KMSKR(aa 600-604); 180 aa at
the C-terminal region are not present in the homologous
enzymes from yeast (Swiss-Prot Accession Number P09436),
tetrahymena (PIR Accession Number A42399) and
methanobacter (Swiss-Prot Accession Number P26499)"
/codon_start=1
/product="isoleucyl-tRNA synthetase"
/protein_id="AAA80153.1"
/translation="MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFY
DGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGI
RGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVW
WVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETV
SLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEIL
ERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYR
VCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATT
FTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLK
DARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTI
PSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRG
WFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALR
LYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNE
NTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRR
RLKGENGMEDCVMALETLFSVLLSLCRLIAPYTPFLTELMYQNLKVLIDPVSVQDKDT
LSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQD
PEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMT
SIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVL
LDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHT
EFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNIC
ANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLS
LSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNG
LTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
"
polyA_site 4508
BASE COUNT 1299 a 947 c 1036 g 1226 t
ORIGIN
1 cgggcagcgt ggaccccgga tgagttgctt ttaggcttgc tggcccgcgg ggctgtccag
61 gcacgcgagg cccctcaggt acgccctctc ttccctgcag gatccggccc tcaaagacga
121 gggtcacgca cgcgttacaa ccccgaaaca gtagcacaag atttaatttt taaaagagcg
181 tgtttcttcg gggcttgccg ttcgttcgtt tccagcctca ggaatttatg gtcgcctttt
241 tgaatgagca acaaaatgct tcaacaagtt ccagaaaaca taaattttcc tgctgaagaa
301 gagaaaatct tggagttttg gactgaattt aattgttttc aggaatgctt aaagcaatca
361 aaacataaac caaaatttac cttctatgat ggtcctcctt ttgcaactgg actgcctcac
421 tatggacata tacttgcggg tacaattaaa gatatagtta caagatatgc tcaccagagt
481 gggtttcatg ttgacagaag atttggatgg gattgccatg gcttacctgt ggaatatgaa
541 attgataaga cactgggaat cagaggacca gaggatgtgg ccaaaatggg gattacagag
601 tataacaatc agtgccgagc aattgtgatg agatattctg ctgagtggaa gtctactgtt
661 agcagacttg gccgatggat tgactttgac aatgactata aaactctgta tccacaattc
721 atggaatcag tctggtgggt cttcaaacaa ctctatgata aaggccttgt ttatagaggt
781 gtgaaagtca tgcccttctc tacggcatgt aacactccac tttccaactt cgagtcacac
841 cagaattata aggatgttca agatccttca gtatttgtaa ctttcccttt ggaagaagat
901 gaaactgtat ctttagttgc ttggacaacc actccctgga ctctacctag taaccttgct
961 gtgtgtgtta atccagaaat gcaatatgtg aaaattaaag atgttgccag aggacgatta
1021 ctcattttaa tggaagccag attgtcagcc ctctataaat tggagagtga ctatgagatc
1081 cttgaaagat ttcctggtgc ctatcttaaa ggcaagaagt acaggcccct gtttgactat
1141 ttcctgaagt gtaaagagaa tggcgctttc actgtgcttg ttgacaacta tgtgaaggaa
1201 gaagaaggca caggggttgt ccaccaagct ccttacttcg gtgctgagga ctatcgggtc
1261 tgtatggact ttaacattat tcggaaagac tcactccctg tttgccctgt ggatgcttca
1321 ggctgcttca caacggaggt gacagatttc gcaggacagt atgtgaagga tgctgacaaa
1381 agtatcatca ggactttgaa ggaacaaggc cgacttctgg ttgccaccac cttcactcac
1441 agctaccctt tttgctggag atcagacact cctctaattt acaaagcagt gcccagctgg
1501 tttgtgcgag tggagaacat ggtggaccag ctcctaagga acaatgacct gtgctactgg
1561 gtcccagagt tggtacgaga aaaacgattt ggaaattggc tgaaagatgc acgtgactgg
1621 acaatttcca gaaacagata ctggggcacc cccatcccac tgtgggtcag cgatgacttt
1681 gaggaggtgg tatgcattgg gtcagtggcg gaacttgaag aactgtcagg agcaaagatc
1741 tcagatctcc acagagagag tgttgaccac ctgaccattc cttcacgctg tgggaaggga
1801 tccttgcacc gcatctctga agtgtttgac tgttggtttg agagtggcag catgccctat
1861 gctcaggttc attacccgtt tgaaaacaag agggagtttg aggatgcttt tcctgcagat
1921 ttcattgccg agggcatcga ccaaaccaga ggatggtttt ataccctgct ggtgctggcc
1981 acggccctct ttggacaacc gcctttcaag aacgtaattg tgaatgggct tgtcctggca
2041 agtgatggcc aaaaaatgag caaacggaaa aagaattatc cagatccagt ttccatcatc
2101 cagaagtatg gtgctgatgc cctcagatta tatctgatta actcccctgt ggtgagagca
2161 gaaaacctcc gctttaaaga agagggtgtg cgggacgtcc ttaaggatgt actgctccca
2221 tggtacaatg cctatcgctt cttaatccag aacgttctga ggctccagaa ggaggaagaa
2281 atagaatttc tctacaatga gaacacggtt agagaaagcc ccaacattac agaccggtgg
2341 atcctgtcct tcatgcagtc tctcattggc ttctttgaga ctgaaatggc agcttatagg
2401 ctttatactg tggtgcctcg cctggtcaag tttgtagata ttctgaccaa ttggtatgtt
2461 agaatgaacc gcagaagatt aaagggtgaa aatgggatgg aggattgtgt catggcccta
2521 gaaaccttgt ttagtgttct gctttctctt tgcagactta tagctcccta cacacctttt
2581 ctcactgaat tgatgtacca gaatctaaag gtgctgattg accctgtttc tgttcaggac
2641 aaggacacac tcagcattca ctacctcatg ctgccccgtg ttcgagaaga attgattgac
2701 aagaaaacag agagtgcagt atctcagatg cagtctgtga ttgaacttgg aagagtgatc
2761 agagaccgaa aaactattcc cataaagtat cctttgaaag aaattgtggt tatccatcaa
2821 gatccagaag ctcttaaaga tatcaagtct ttggagaagt atatcattga ggaactcaat
2881 gttcgaaaag ttacactgtc tacagataaa aacaagtatg gcattcggct aagggcagaa
2941 ccagatcaca tggtcctggg gaagcgtctg aagggagcct ttaaggcagt gatgacgtcc
3001 atcaagcagt tgagcagtga ggagctggag cagttccaga agactgggac cattgttgtg
3061 gaaggccatg aattgcacga tgaagacatc cgcctcatgt acacctttga tcaggccaca
3121 ggtgggactg cgcaatttga agcacactca gatgctcagg ctttggtcct cttagatgtc
3181 actcctgacc agtcaatggt agatgaagga atggctcggg aagtcatcaa tcgcatacag
3241 aaacttcgca aaaagtgcaa tctggttcca actgatgaaa tcacagtgta ctataaagca
3301 aagtctgaag gaacatatct gaatagtgtt attgaaagcc acacagagtt catatttacc
3361 accataaagg ctcccttgaa accatatcca gtttctccat cggataaagt ccttattcaa
3421 gaaaaaacac agttgaaggg atctgaactg gaaattacac tcaccagagg atcttccctt
3481 cctggtcctg cttgtgcata tgtcaatctt aacatttgtg caaatggcag tgaacaaggt
3541 ggagtattgc tcctggaaaa tccaaaaggt gacaataggt tggacctttt aaagctgaag
3601 agtgttgtca ctagcatttt tggtgtgaaa aatacagagc tggctgtctt ccatgatgaa
3661 acagaaatac aaaaccaaac tgacttactg agtcttagtg gaaaaacact ttgtgtgact
3721 gcaggatcgg ctccctctct gatcaacagt tctagtactc ttctttgtca gtatatcaac
3781 ctacagctcc tgaatgcaaa gccacaagag tgtttaatgg ggacagtggg cactctcctg
3841 cttgaaaacc cacttgggca gaatggactc acccaccaag gtcttctgta tgaagcagcc
3901 aaggtgtttg gccttcggag caggaagcta aagctgtttc tgaatgagac ccaaacgcag
3961 gaaattacag aagacatccc cgtgaagact ttgaatatga agactgtgta tgtttctgtg
4021 ttaccaacaa cagcagactt ctagcatgta cttatcaatg ttgttcggtc agcccttccc
4081 taattacacc tatcccctac acatacatgc acatagacac acacatgaac acactgaaga
4141 tatttccttc aggtgtgtgt aaaatatgct gcttggattg aaattcaaat gggattgatt
4201 agtcaagtaa cttgagacct cacagtaatc ttcacactta accttagaca cctatgcagt
4261 catgttggga gcaggttaca atgttacttc agcccacagt ttatttctat tcttgagttc
4321 ttaagtacag aagatagaag tgatttaaat ggcatagtat atatatcatt ttctggcctt
4381 ttaaaattta tttgagacct cttgatgaaa tggacatatt atatatttct gccacctgga
4441 ttttcctgga taatttgatg gaatatttta agtttcagta aatcagaaca ataaacaaac
4501 tcagatat
//