LOCUS HSU04641 3953 bp mRNA linear HUM 16-MAY-1996
DEFINITION Human pyruvate carboxylase (PC) mRNA, complete cds.
ACCESSION U04641
VERSION U04641.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3753)
AUTHORS Wexler,I.D., Du,Y., Lisgaris,M.V., Mandal,S.K., Freytag,S.O.,
Yang,B.S., Liu,T.C., Kwon,M., Patel,M.S. and Kerr,D.S.
TITLE Primary amino acid sequence and structure of human pyruvate
carboxylase
JOURNAL Biochim. Biophys. Acta 1227 (1-2), 46-52 (1994)
PUBMED 7918683
REFERENCE 2 (bases 1 to 3953)
AUTHORS Wexler,I.D.
TITLE Direct Submission
JOURNAL Submitted (21-DEC-1993) Isaiah D. Wexler, Pediatrics, Case Western
Reserve University, 2047 Abington Road, Cleveland, OH 44106, USA
FEATURES Location/Qualifiers
source 1..3953
/db_xref="H-InvDB:HIT000217557"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="liver and kidney"
/clone_lib="lambda GT11 libraries"
/dev_stage="adult"
gene 1..3953
/gene="PC"
mRNA <1..>3953
/gene="PC"
5'UTR 1..38
/gene="PC"
CDS 39..3575
/gene="PC"
/EC_number="6.4.1.1"
/experiment="experimental evidence, no additional details
recorded"
/codon_start=1
/product="pyruvate: carbon-dioxide ligase (ADP-forming)"
/protein_id="AAA99537.1"
/translation="MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVAN
RGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDI
IKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAI
AAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYT
RAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVE
IAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHT
VTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTG
RIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEF
RVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVM
VNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLM
DTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPW
RRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNY
LPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCI
KDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAA
DSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKS
GNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIV
GDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLP
RVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPL
DSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSIL
VKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKME
TVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE"
sig_peptide 39..98
/gene="PC"
mat_peptide 99..3572
/gene="PC"
/product="pyruvate: carbon-dioxide ligase (ADP-forming)"
/EC_number="6.4.1.1"
/function="carboxylation of pyruvate"
/note="pyruvate carboxylase"
misc_feature 3462..3473
/gene="PC"
/experiment="experimental evidence, no additional details
recorded"
/note="encodes biotin binding site"
/function="lysine residue binds biotin"
3'UTR 3576..3953
/gene="PC"
regulatory 3928..3933
/regulatory_class="polyA_signal_sequence"
/gene="PC"
polyA_site 3953
/gene="PC"
/note="19 A nucleotides"
BASE COUNT 808 a 1219 c 1213 g 713 t
ORIGIN
1 gggttcttac ccatttaaag tttgaccaaa cactaaggat gctgaagttc cgaacagtcc
61 atgggggcct gaggctcctg ggaatccgcc gaacctccac cgcccccgct gcctccccaa
121 atgtccggcg cctggagtat aagcccatca agaaagtcat ggtggccaac agaggtgaga
181 ttgccatccg tgtgttccgg gcctgcacgg agctgggcat ccgcaccgta gccatctact
241 ctgagcagga cacgggccag atgcaccggc agaaagcaga tgaagcctat ctcatcggcc
301 gcggcctggc ccccgtgcag gcctacctgc acatcccaga catcatcaag gtggccaagg
361 agaacaacgt agatgcagtg caccctggct acgggttcct ctctgagcga gcggacttcg
421 cccaggcctg ccaggatgca ggggtccggt ttattgggcc aagcccagaa gtggtccgca
481 agatgggaga caaggtggag gcccgggcca tcgccattgc tgcgggtgtt cccgttgtcc
541 ctggcacaga tgcccccatc acgtccctgc atgaggccca cgagttctcc aacacctacg
601 gcttccccat catcttcaag gcggcctatg ggggtggagg gcgtggcatg agggtggtgc
661 acagctacga ggagctggag gagaattaca cccgggccta ctcagaggct ctggccgcct
721 ttgggaatgg ggcgctgttt gtggagaagt tcatcgagaa gccacggcac atcgaggtgc
781 agatcttggg ggaccagtat gggaacatcc tgcacctgta cgagcgagac tgctccatcc
841 agcggcggca ccagaaggtg gtcgagattg cccccgccgc ccacctggac ccgcagcttc
901 ggactcggct caccagcgac tctgtgaaac tcgctaaaca ggtgggctac gagaacgcag
961 gcaccgtgga gttcctggtg gacaggcacg gcaagcacta cttcatcgag gtcaactccc
1021 gcctgcaggt ggagcacacg gtcacagagg agatcaccga cgtagacctg gtccatgctc
1081 agatccacgt ggctgagggc aggagcctac ccgacctggg cctgcggcag gagaacatcc
1141 gcatcaacgg gtgtgccatc cagtgccggg tcaccaccga ggaccccgcg cgcagcttcc
1201 agccggacac cggccgcatt gaggtgttcc ggagcggaga gggcatgggc atccgcctgg
1261 ataatgcttc cgccttccaa ggagccgtca tctcgcccca ctacgactcc ctgctggtca
1321 aagtcattgc ccacggcaaa gaccacccca cggccgccac caagatgagc agggcccttg
1381 cggagttccg cgtccgaggt gtgaagacca acatcgcctt cctgcagaat gtgctcaaca
1441 accagcagtt cctggcaggc actgtggaca cccagttcat cgacgagaac ccagagctgt
1501 tccagctgcg gcctgcacag aaccgggccc aaaagctgtt gcactacctc ggccatgtca
1561 tggtaaacgg tccaaccacc ccgattcccg tcaaggccag ccccagcccc acggaccccg
1621 ttgtccctgc agtgcccata ggcccgcccc cggctggttt cagagacatc ctgctgcgag
1681 aggggcctga gggctttgct cgagctgtgc ggaaccaccc ggggctgctg ctgatggaca
1741 cgaccttcag ggacgcccac cagtcactgc tggccactcg tgtgcgcacc cacgatctca
1801 aaaagatcgc cccctatgtt gcccacaact tcagcaagct cttcagcatg gagaactggg
1861 gaggagccac gtttgacgtc gccatgcgct tcctgtatga gtgcccctgg cggcggctgc
1921 aggagctccg ggagctcatc cccaacatcc ctttccagat gctgctgcgg ggggccaatg
1981 ctgtgggcta caccaactac ccagacaacg tggtcttcaa gttctgtgaa gtggccaaag
2041 agaatggcat ggatgtcttc cgtgtgtttg actccctcaa ctacttgccc aacatgctgc
2101 tgggcatgga ggcggcagga agtgccggag gcgtggtgga ggctgccatc tcatacacgg
2161 gcgacgtggc cgaccccagc cgcaccaagt actcactgca gtactacatg ggcttggccg
2221 aagagctggt gcgagctggc acccacatcc tgtgcatcaa ggacatggcc gggctgctga
2281 agcccacggc ctgcaccatg ctggtcagct ccctccggga ccgcttcccc gacctcccac
2341 tgcacatcca cacccacgac acgtcagggg caggcgtggc agccatgctg gcctgtgccc
2401 aggctggagc tgatgtggtg gatgtggcag ctgattccat gtctgggatg acttcacagc
2461 ccagcatggg ggccctggtg gcctgtacca gagggactcc cctggacaca gaggtgccca
2521 tggagcgcgt gtttgactac agtgagtact gggagggggc tcggggactg tacgcggcct
2581 tcgactgcac ggccaccatg aagtctggca actcggacgt gtatgaaaat gagatcccag
2641 ggggccagta caccaacctg cacttccagg cccacagcat ggggcttggc tccaagttca
2701 aggaggtcaa gaaggcctat gtggaggcca accagatgct gggcgatctc atcaaggtga
2761 cgccctcctc caagatcgtg ggggacctgg cccagtttat ggtgcagaat ggattgagcc
2821 gggcagaggc cgaagctcag gcggaagagc tgtcctttcc ccgctccgtg gtggagttcc
2881 tgcagggcta catcggtgtc ccccatgggg ggttccccga accctttcgc tctaaggtac
2941 tgaaggacct gccaagggtg gaggggcggc ctggagcctc cctccctccc ctggatctgc
3001 aggcactgga gaaggagctg gtagaccggc atggggagga ggtgacgccg gaagatgtgc
3061 tctcagcagc tatgtacccc gatgtgtttg cccacttcaa ggacttcact gccacctttg
3121 gccccctgga tagcctgaat actcgcctct tcctgcaggg acccaagatc gcagaggagt
3181 ttgaggtgga gctggagcgg ggcaagacgc tgcacatcaa agccctggcc gtgagcgacc
3241 tgaaccgggc cggccagagg caggtcttct ttgagctcaa tgggcagctg cggtccatct
3301 tggtcaagga cacccaggcc atgaaggaga tgcacttcca ccccaaggcc ctaaaggacg
3361 tgaagggcca gatcggggcg cccatgcctg ggaaggtgat agacatcaaa gtggtggcag
3421 gggccaaggt ggccaagggc cagcccctgt gtgtgctcag tgccatgaag atggagactg
3481 tggtgacctc acccatggag ggtactgtcc gcaaggttca tgtgaccaag gacatgacac
3541 tggaaggtga cgacctcatc ctggagatcg agtgatcttg ccccagaccg gcagcctggc
3601 catccccaag ccttcaacag aagctgtgct gccacggcag gcccaggcca gccagtgccc
3661 gaggccagga aggccgggcc gtggaggtcc tgtgtccaca gctggacagg agagacaccg
3721 cctgcggtgg ttcattcctt tcagccatcg tcctttcctc cggcggacag ctgcttacat
3781 gttcatctct tgccaaataa gggtcccctc ctcactggag actacaagtg gtgggtcagg
3841 tggtcctagg acccagggga ggtttagggg tcctatctcc tgggggaagg ggagatcaag
3901 atgtcccagg tcctgggaag tttactcaat aaagctggct ttcccctgcc ctc
//