LOCUS HSU02388 2369 bp mRNA linear HUM 21-JUL-2000
DEFINITION Homo sapiens cytochrome P450 4F2 (CYP4F2) mRNA, complete cds.
ACCESSION U02388
VERSION U02388.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2369)
AUTHORS Chen,L. and Hardwick,J.P.
TITLE The human liver CYP4F2 cDNA sequence and expression in
Baculovirus-infected insect cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2369)
AUTHORS Chen,L. and Hardwick,J.P.
TITLE Identification of a new P450 subfamily, CYP4F1, expressed in rat
hepatic tumors
JOURNAL Arch. Biochem. Biophys. 300 (1), 18-23 (1993)
PUBMED 8424651
REFERENCE 3 (bases 1 to 2369)
AUTHORS Kikuta,Y., Kusunose,E., Endo,K., Yamamoto,S., Sogawa,K.,
Fujii-Kuriyama,Y. and Kusunose,M.
TITLE A novel form of cytochrome P-450 family 4 in human
polymorphonuclear leukocytes: cDNA cloning and expression of
leukotriene B4 omega-hydroxylase, D12620 and D12621
JOURNAL Journal of Biochemistry 268, 9376-9380 (1993)
REFERENCE 4 (bases 1 to 2369)
AUTHORS Hardwick,J.P.
TITLE Direct Submission
JOURNAL Submitted (05-OCT-1993) James P. Hardwick, Northeastern Ohio
Universities College of Medicine, 4209 State Rt. 44, Rootstown, OH
44272, USA
REFERENCE 5 (bases 1 to 2369)
AUTHORS Hardwick,J.P.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2000) James P. Hardwick, Northeastern Ohio
Universities College of Medicine, 4209 State Rt. 44, Rootstown, OH
44272, USA
REMARK Sequence update by submitter
COMMENT On Jul 21, 2000 this sequence version replaced U02388.1.
FEATURES Location/Qualifiers
source 1..2369
/db_xref="H-InvDB:HIT000217458"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="C13"
/sex="male"
/cell_type="hepatocytes"
/clone_lib="Uni-zap CFHL"
gene 1..2369
/gene="CYP4F2"
5'UTR 1..59
/gene="CYP4F2"
CDS 60..1622
/gene="CYP4F2"
/function="fatty acid hydroxylase; eicosanoid metabolism"
/note="expressed in hepatic tumors"
/codon_start=1
/product="cytochrome P450 4F2"
/protein_id="AAC50052.2"
/translation="MSQLSLSWLGLCRVAASPWLLLLLVGASWLLAHVLAWTYAFYDN
CRRLRCFPQPPRRNWFWGHQGMVNPTEEGMRVLTQLVATYPQGFKVWMGPISPLLSLC
HPDIIRSVINASAAIAPKDKFFYSFLEPWLGDGLLLSAGDKWSRHRRMLTPAFHFNIL
KPYMKIFNESVNIMHAKWQLLASEGSACLDMFEHISLMTLDSLQKCVFSFDSHCQEKP
SEYIAAILELSALVSKRHHEILLHIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRR
TLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTA
SVLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAHLPFLTMCMKESLRVH
PPVPVISRHVTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPENI
KERSPLAFIPFSAGPRNCIGQTFAMAEMKVVLALTLLRFRVLPDHTEPRRKPELVLRA
EGGLWLRVEPLS"
misc_feature 1128..1151
/gene="CYP4F2"
/note="Region: 4F subfamily specific region 4Fa"
misc_feature 1194..1223
/gene="CYP4F2"
/note="Region: 4F subfamily specific region 4Fb"
misc_feature 1461..1463
/gene="CYP4F2"
/note="heme; binding site"
misc_feature 1008..1056
/gene="CYP4F2"
/note="family 4 consensus sequence in the translated
peptide"
3'UTR 1623..2369
/gene="CYP4F2"
BASE COUNT 568 a 648 c 598 g 555 t
ORIGIN
1 ctcggatcag tctggcagag agaggaggtt gtctgggaca gactgctcct gacagaagga
61 tgtcccagct gagcctgtcc tggctgggcc tctgccgagt ggcagcatcc ccttggctgc
121 tcctcctgct ggtcggggcc tcctggctcc tggcccatgt cctggcctgg acctacgcct
181 tctatgacaa ctgccgccgc cttcggtgtt tcccacaacc cccaagacgg aactggtttt
241 ggggacacca gggcatggtc aaccccacag aggagggcat gagagttctg actcagctgg
301 tggccaccta cccccagggc tttaaggtct ggatgggacc catctccccc ctcctcagtt
361 tgtgccaccc cgacatcatc cggtctgtca tcaacgcctc agctgccatt gcaccaaagg
421 acaagttctt ctacagcttc ctggagccct ggctggggga tgggctcctg ctgagtgctg
481 gtgacaagtg gagccgccac cgtcggatgc tgacgcctgc cttccatttc aacatcctga
541 agccctatat gaagattttc aatgagagtg tgaacatcat gcacgccaag tggcagctcc
601 tggcctcaga gggtagtgcc tgtttggata tgtttgagca catcagcctc atgaccttgg
661 acagtctaca gaaatgtgtc ttcagctttg acagccattg tcaggagaaa cccagtgaat
721 atattgccgc catcttggag ctcagtgccc ttgtatcaaa aagacaccat gagatcctcc
781 tgcatattga cttcctgtat tatctcaccc ctgatgggca gcgtttccgc agggcctgcc
841 gcctggtgca cgacttcaca gatgccgtca tccaggagcg gcgccgcact ctccctagcc
901 agggtgttga tgacttcctc caagccaagg ccaaatccaa gactttggac ttcattgatg
961 tactcctgct gagcaaggat gaagacggga agaagttatc tgatgaggac ataagagcag
1021 aagctgacac ctttatgttt gagggccatg acaccacggc cagtgtcctc tcctgggtcc
1081 tgtaccacct tgcaaagcac ccagaatacc aggagcgctg ccggcaggag gtgcaagaac
1141 ttctgaagga ccgtgagcct aaagagattg aatgggacga cctggcccat ttgcccttcc
1201 tgaccatgtg catgaaggag agcctgcgcg tgcatccccc agtcccggtc atctcccgcc
1261 atgtcaccca ggacattgtg ctcccagacg gccgggtcat ccccaaaggc attatctgcc
1321 tcatcagtgt tttcggaacc catcacaacc cagctgtgtg gccggaccct gaggtctacg
1381 acccctttcg ctttgaccca gagaacatca aggagaggtc acctctggct tttattccct
1441 tctcggcagg gcccaggaac tgcatcgggc agacgttcgc gatggcggag atgaaggtgg
1501 tcctggcgct cacgctgctg cgcttccgcg tcctgcctga ccacaccgag ccccgcagga
1561 agccggagct ggtcctgcgc gcagagggcg gactttggct gcgggtggag cccctgagct
1621 gagttctgca gagacccact ctgaccccac taaaatgacc cctgattcat caaaagtgaa
1681 gcctagaatt accctaagac cctgttccac agtcctgtat tccatcctag atatctactc
1741 aaaataattg agacaagtgt tcaaacagaa agacgcttgt gcgtgaatgt tcatggcggc
1801 cctattcaca gtagccaaac gatgaaaaca accccaagct atatattacc agatgaaagg
1861 ataaacaaaa tgtggtccat ccatacaatg gagtattaca cagccataaa aaggaatgaa
1921 gcagtgatcc ctactacact gtggatgaac cttgaatgca tgatactgaa tgaaagacgt
1981 cagatgcaaa aggtcacata gtgtactgtc cttttatacg aaatttccag aacaggccaa
2041 tctgaagaga tgcatagcgg attggtggct ttcagcagct gtggggaggt gggactgagg
2101 agcgactgct aatcagtatg gggtttcctc ccgggatggt gaaaatgttc cggacctaga
2161 tactgacgaa ggtagcacga cactgtgagt gcactaaatg ctattgaatt ggacactttg
2221 aaatggtgaa tttcgtggta tgtgaattct acctcaatca aaaaaatttg ctattttatc
2281 tcacatacat tttttttctg tccaggttgt tcatataata atatgctgtg agcatctttc
2341 catgacatta aatcatctta ggaaacatt
//