LOCUS MG462882 7276 bp DNA circular SYN 04-JAN-2018
DEFINITION Cloning vector PVRL1, complete sequence.
ACCESSION MG462882
VERSION MG462882.1
KEYWORDS .
SOURCE Cloning vector PVRL1
ORGANISM Cloning vector PVRL1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7276)
AUTHORS Lucidi,M., Runci,F., Rampioni,G., Frangipani,E., Leoni,L. and
Visca,P.
TITLE New shuttle vectors for gene cloning and expression in
multidrug-resistant Acinetobacter species
JOURNAL Antimicrob. Agents Chemother. (2018) In press
REFERENCE 2 (bases 1 to 7276)
AUTHORS Lucidi,M., Runci,F., Rampioni,G., Frangipani,E., Leoni,L. and
Visca,P.
TITLE Direct Submission
JOURNAL Submitted (10-NOV-2017) Department of Science, Roma Tre University,
Viale Marconi 446, Rome, Roma 00146, Italia
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7276
/organism="Cloning vector PVRL1"
/mol_type="other DNA"
/db_xref="taxon:2067737"
/note="Acinetobacter sp. - Escherichia coli shuttle
vector"
gene complement(527..1060)
/gene="aacC1"
CDS complement(527..1060)
/gene="aacC1"
/note="gentamicin resistance protein"
/codon_start=1
/transl_table=11
/product="gentamicin 3-N-acetyltransferase"
/protein_id="AUJ88094.1"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
GIREEVMHFDIDPSTAT"
regulatory complement(1249..1277)
/regulatory_class="promoter"
/note="class 1 integron promoter"
regulatory complement(1295..1399)
/regulatory_class="promoter"
/note="beta-lactamase promoter"
CDS complement(1489..1854)
/note="allows blue/white screening in Escherichia coli
DH5alpha"
/codon_start=1
/transl_table=11
/product="beta-galactosidase LacZalpha"
/protein_id="AUJ88092.1"
/translation="MTMITPSAQLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPL
VLERPPPRWSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWKL"
primer_bind 1638..1654
/note="M13 forward, universal sequencing primer"
regulatory 1664..1682
/regulatory_class="promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 1715..1731
/note="SK primer, pBluescript II universal sequencing
primer"
primer_bind complement(1765..1781)
/note="KS primer, pBluescript II universal sequencing
primer"
regulatory complement(1811..1829)
/regulatory_class="promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1850..1866)
/note="M13 reverse, universal sequencing primer"
regulatory complement(1874..1890)
/regulatory_class="other"
/note="operator; lac gene operator; lacI repressor binding
site"
regulatory complement(1898..1928)
/regulatory_class="promoter"
/note="lac promoter"
regulatory complement(1943..1964)
/regulatory_class="promoter"
/note="CAP binding site"
regulatory complement(2252..2840)
/regulatory_class="replication_regulatory_region"
/note="ColE1-like origin of replication"
regulatory 3164..3193
/regulatory_class="promoter"
/note="putative MobA/MobL gene predicted promoter"
CDS 3317..3877
/note="contains MobA/MobL family domain; may be involved
in plasmid mobilization"
/codon_start=1
/transl_table=11
/product="putative MobA/MobL protein"
/protein_id="AUJ88093.1"
/translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDD
KKKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQ
KYEQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLE
NERQPSFKPRYDQEQPKPKKDNDLTF"
regulatory 3861..3888
/regulatory_class="promoter"
/note="putative antitoxin-toxin module-predicted promoter"
CDS 3922..4215
/note="putative component of an antitoxin-toxin system
involved in plasmid stability and maintenance"
/codon_start=1
/transl_table=11
/product="putative PaaA2-like antitoxin"
/protein_id="AUJ88095.1"
/translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQ
QEAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
CDS 4205..4474
/note="putative component of an antitoxin-toxin system
involved in plasmid stability and maintenance"
/codon_start=1
/transl_table=11
/product="putative ParE2-like toxin"
/protein_id="AUJ88096.1"
/translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVD
NRLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
regulatory 4238..5574
/regulatory_class="replication_regulatory_region"
/note="minimal origin of replication for Acinetobacter
sp."
regulatory 5747..5773
/regulatory_class="promoter"
/note="putative RCR protein gene predicted promoter"
CDS 5826..6101
/note="contains a domain belonging to RepB proteins; may
be involved in rolling circle replication (RCR)"
/codon_start=1
/transl_table=11
/product="putative RCR protein"
/protein_id="AUJ88097.1"
/translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLD
SQRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
regulatory 6150..6170
/regulatory_class="terminator"
/note="bidirectional Rho-independent transcriptional
terminator"
CDS complement(6189..6458)
/note="contains MobA/MobL family domain; may be involved
in plasmid mobilization"
/codon_start=1
/transl_table=11
/product="putative MobA/MobL protein"
/protein_id="AUJ88098.1"
/translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQAR
SDDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
regulatory complement(6491..6516)
/regulatory_class="promoter"
/note="putative MobA/MobL gene predicted promoter"
regulatory 6627..6653
/regulatory_class="promoter"
/note="putative nickase-like gene predicted promoter"
CDS 6743..7216
/note="may be involved in rolling circle replication
(RCR)"
/codon_start=1
/transl_table=11
/product="putative nickase-like protein"
/protein_id="AUJ88099.1"
/translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDY
TKKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPH
ELDKTQRQALVTELCQSLVKAYGVAVDVAIHAPHVHGGRRKKPHAHIRHRRQATC"
BASE COUNT 2202 a 1573 c 1692 g 1809 t
ORIGIN
1 cccctcatgt gcatggggga agtgatgaaa agaaacccat cacgcccaca tactaatgac
61 aacaagaagc cttacaccgc aagggctagg gaagaaagtc agggagctag atcagaactc
121 aacgctgaaa aaaatcaggg aaaaagtggc ggatctgacg aacgtccacc tagagcgagc
181 agggcttgag gtgagagttg atcaccgcag tcataaggat cgtggactag acatcgagcc
241 gacattcaag gagggacttg aagccaccca cgccaagcgc aggggggagg aactggagat
301 cgtcaagcgt aatgatgaaa tcaaaaagcg caataaaaaa gctgaaaggc ttgatatgtc
361 tatcattgag acgagcaaca gagtagagca attgacactt gaacagcagc aacagcagca
421 ggggctgcat acttggtctg acaatcgatg cgaattggcc gcggcgttgt gacaatttac
481 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
541 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
601 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
661 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
721 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
781 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
841 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
901 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
961 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
1021 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
1081 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
1141 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
1201 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
1261 gaacaggctt atgtcaattc ggttgaatac tcatactctt cctttttcaa tattattgaa
1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
1441 ttattatcat gacattaacc tataaaaata ggcgtatcac gaccatggtt acaatttcca
1501 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
1561 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
1621 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
1681 gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct
1741 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
1801 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
1861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
1921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
1981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
2041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
2101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
2161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
2221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
2281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2461 ggtatctctg ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
2701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
3001 gtgatccccc ctggaggatg ccgtagacca agcccccgca gtctatgcca ccgcacaaca
3061 ggcactatac gaccaccaac aacaccgcca aacgcttata gatgtgtatt tgattgaagt
3121 aaaaacggat gagaagcgag cagagcatag attttacaag gctttgcaag acaatatcga
3181 ccgcttgcag cttgataaag tgatcaaaga gggacgagca gctacagcac tgctaaggga
3241 gatcggagag ccactgccgc caccagtgaa agtgaaaaca ggttggttta gtgctgatta
3301 ttacggcttt gaggacatgc tcacacgttg ggataatgat caatctagcc tttccattta
3361 tcacagagag ataaaagagg agcaacagga acagcaacga aaacaacagc agatagaaag
3421 agaaactaaa caaaaacagg aagatgacaa aaagaaacgg caggactatg aaagatattt
3481 atcaaaatac ggctatgtca aagatgctga gtatgaccat atcagcgacc atgttgcttc
3541 agacatttcc atgtttacac gtcttcaggc gcaggcatgg gcaagtaacg atatgcaaga
3601 gtacataagg tgttcaaagc aaaaatacga gcagatcagc gacagaatag cctatgagcg
3661 tgacttgaaa aaaatcgggc aattgagccg tatcctggat aaagacagac aggcacttgc
3721 tgatatttta ccgcagttga tgccttacta tgagcagatg caacaggctg ttaataaacg
3781 gaaaatttta cttgagaatg agcgacagcc atcatttaaa ccacgttatg accaagaaca
3841 gcctaagccg aaaaaagaca atgacctaac tttttaatgt gttacaatag cattcaaatg
3901 taacacattg gagtgaaaaa catgacagaa gcgactttta cctttcgggt agaccatgac
3961 ctaaagcaag aattttcaag ccttgctaag actgttgatc ggtcgggggc gcagctaatc
4021 cgtgacttca tgcgtgactt tgttaagaag caacaggaag ccgcagacta tgacaaatgg
4081 ttcaagcagc aggtacagat cggcttaaat gaagccaatg caggcaagtt gattccacat
4141 gaggacgtaa aggcagagtt tgccgctaga cgtgctgcaa cattggcgaa actggcagcg
4201 aagaatgaag attgagtgga ctgaaacagc tcgccaagat cgtagaaata tctatgatta
4261 tcttgaagaa cgcaacccta tagcagctat tgaaattgat gatttaattg aagaaaagac
4321 agatttactt gttgataatc gactgatggg gcgcacaggc agacagaaag atactaggga
4381 gttagtgata catccgcatt atgtggttgt atatgacatc actgatataa tacggatact
4441 cagagtgcta cacacatcgc aggagtggtc atgacttact catgtacttt ggattattta
4501 gtgttataaa atcctgattt ataaattttt tttgttaaaa aagataaaag ccccttgcaa
4561 ttgcttgggg ctttaccgta atttatgggg tacagatctt cgatactgac atatcggcaa
4621 tcgaaagcat taaggtttga cgaccgctaa tgatttcacc acaggggctt aatgtacctg
4681 tcttaaattc taaggtttta actcgctttg tcaagcatag accccaaaaa tttagccaat
4741 gtctgtaact caatctgtcc atgtgtgggt gatgaggtac agtgacgcta gcacacatcg
4801 gaaaaacgct attactaggg gaactgaaca gagtagcgga cgcaatgagt agtcatttaa
4861 ttggcggtta tgagcgtgtt caggcggtgc tatcaatcgt aatcataaca gtggcagctt
4921 gatacagtga tgtcatccct gatgcgaaag cgaccgaccg acggtacatc gaatgggaat
4981 actttagggt gatttttaag aatcgctcta gggtgagtat ttcccattca gctctgctcc
5041 ctccctctgg tactttaatc aaaagcacta ctaaacatat gtttttaaat aaaaaatatt
5101 gatatagaga taatattagt aagaataatt aaacaattga atatagataa atcattgtta
5161 aataaagatt aattattaaa atgaatgtat acttatatat aaatcaatga tttaaaatat
5221 ttgataaaga aaacttttca aaaaaaatat aattgagatt gtgtcatttc ggtcaattct
5281 taatatgttc cacgcaagtt ttagctatgg tgctaaacag aaatttgctg aaaaagaact
5341 tttcactgaa ctggttaaaa tgtaagcagc ctgagagccg ccaaaaattt taaaaacaaa
5401 ccgccttaat catcttcaaa aaatacctct aaaacctcac catttgcgtt ttaagaccca
5461 tatttcatcc tgcccttatg ttcccatgct gatagctata aagtgtctgt aatcgcttcc
5521 tatgacgttc taggctgttg ataacttttg gaacaacgca aaatgttaaa atccgatcat
5581 tttttaacct agttattttc gttacagggt aactttggta gtattatttc aatatttagt
5641 tgttacagga taacaaaata tatgttacag ggtaattaat ttagcgttac aggataacta
5701 tagttacagg gtaacgttaa atagttatcc tgtaatttaa aaatagttgc aggatagcat
5761 aaatttttac aaataaattt tataaaaagt tatcctgtaa ctaaaaacaa tgttatcctg
5821 taactatgat atactatatg ggagttaaag acatgaaaga cccgaacgat cagaaaacac
5881 aggacatgct aaaagagccg ccaaaaacca atgccgaacg acaaaaagca tatcgtgaaa
5941 aacgcaaaag ccttgatagt caacgtttgg aggtgtttat agataagggt gtgtcggata
6001 tgcttgcgga catggtgggc gcagcagggg agagccaaaa agccattttg accgcattga
6061 ttgagaaaga gtataagcgg ctgtatgcag tgaaaaaata gcaagctaaa aaagtacatt
6121 gattcaaaaa gtaatgcaga taaaaaagaa accctcgatt tatgagggtt ttttataact
6181 actaatgcct atgaaaataa gtgcttatca ttttctctaa tgtacccaat aaaatcgggg
6241 ttttgttcca caagtttctt gatgtcatta tcatgtttct tcagtaagta agccccgatc
6301 aggatcttgc gttttgcatc gtctgacctt gcctgttttt gctctcgacc tttcaccaca
6361 gcgtcagcta gatcctttct tgccttttcc tgacgttcca atttttcctt ttcttcatcg
6421 agcatttgac ccaattccat agctgttttt ttgaccattt ttaatgcctg tgtctcaacc
6481 tattttccga agtatgaacc atcatcaatg tgaaaagtgt tctacttcaa aaactcgact
6541 tacatcaaga tccgagcgca gcgagcaaaa tcaaaaaaca aagtcaaagc cttattgctc
6601 ttgcttttgc ttttagcctc gcagagttcc cgaagggcgc acttacgcaa aatttttgct
6661 acgccaaatt ttgcaagtac ggtcagggaa accccgacac cccaaccgcc caaaacttgg
6721 ggcggctgtt aataaacagg tgatgaaaat ggcaatttac cattgtgaaa tgcagaacat
6781 ttcgaggtca gatggtcgct caatcgtggc atgtgcagca taccgagcag gcgaaaaatt
6841 gtactgtgat acgtacggaa aagagcagga ctacaccaaa aaaacaggca ttgaatacac
6901 ccaaattttt gccccaactg gggcaagtcc tgacatgtta gatcgtcaaa ccctatggaa
6961 tcgggtagag caatccgaac taaaaaagaa cggtgacatc aaacaggagg caagattagc
7021 aaaggaagta gagatagcat tgccgcatga actggataag acacagcgtc aggcacttgt
7081 taccgagttg tgccagtcct tagttaaagc ctatggagtg gcggtggacg tagcgatcca
7141 tgcccctcat gtgcatgggg gaaggagaaa gaaacctcac gcccacataa gacacaggcg
7201 tcaggcaact tgttaaccga gttgtgccag tccttagtta aagcctatgg agtggcggtg
7261 gacgtagcga tccatg
//