LOCUS HUMLSPRO 2622 bp mRNA linear HUM 27-APR-1993
DEFINITION Human lymphocyte surface protein exons 1-5, complete cds.
ACCESSION M99578
VERSION M99578.1
KEYWORDS lymphocyte surface protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2622)
AUTHORS Voland,J.R., Wyzykowski,R.J., Huang,M. and Dutton,R.W.
TITLE Cloning and sequencing of a trophoblast-endothelial-activated
lymphocyte surface protein: cDNA sequence and genomic structure
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10425-10429 (1992)
PUBMED 1438229
COMMENT Original source text: Homo sapiens (library: lambda gt11) neonate
placenta cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..2622
/db_xref="H-InvDB:HIT000197044_03"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_type="trophoblast"
/tissue_type="placenta"
/dev_stage="neonate"
/tissue_lib="lambda gt11"
mRNA join(1..153,153..933,934..1082,1083..1323,1324..2622)
5'UTR 1..171
/note="84% GC in 5'UTR"
exon 1..153
/note="codes the 5' UTR; intron between exon 1 and exon 2
is 2.7Kb"
/number=1
exon 153..933
/note="codes the extracellular domain; intron between exon
2 and exon 3 is 0.7Kb"
/number=2
CDS 170..1822
/note="550 amino acids MW=61kDa, glycosylated=75 kDa;
expressed on endothelium, activated lymphocytes and
syncytiotrophoblast, contains leucine zipper and basic
region homologous to myc; 721P"
/codon_start=1
/protein_id="AAA36187.1"
/translation="MAAATIVHDTSEAVELCPAYGLYLKPITKMTISVALPQLKQPGK
SISNWEVMERLKGMVQNHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLACLDGKTIK
LSGFSDILKVRAAEFKIDFPTRHDWDSFFRDAKDMNETLPGERPDTIHLEGLPCKWFA
LKESGSEKPSEDVLVKVFEKFGEIRNVDIPMLDPYREEMTGRNFHTFSFGGHLNFEAY
VQYREYMGFIQAMSALRGMKLMYKGEDGKAVACNIKVSFDSTKHLSDASIKKRQLERQ
KLQELEQQREEQKRREKEAEERQRAEERKQKELEELERERKREEKLRKREQKQRDREL
RRNQKKLEKLQAEEQKQLQEKIKLEERKLLLAQRNLQSIRLIAELLSRAKAVKLREQE
QKEEKLRLQQQEERRRLQEAELRRVEEEKERALGLQRKERELRERLLSILQSKKPDDS
HTHDELGVAHGPAAARPGHPADRVVRLCERHHAAPPRGPAPGRCPQGEPGPPRGRRRS
QKRERERGRGGPMQGGSELLSCGPRGWLSREEVPGRRPLLHS"
misc_feature 172..232
/note="codes for protein leader sequence"
exon 934..1082
/note="codes the putative basic region; intron between
exon 3 and exon 4 is 3.5Kb"
/number=3
exon 1083..1323
/note="codes the leucine zipper region; the intron between
exon 4 and exon 5 is 1.3Kb"
/number=4
misc_feature 1199..1263
/note="codes for leucine zipper, homologous to myc"
exon 1324..2622
/note="codes the serine phosphorylation site and the
3'UTR"
/number=5
BASE COUNT 589 a 792 c 898 g 343 t
ORIGIN
1 ggcgacggcg gtggcggcgt cggaggcgcc tccgggggac ggtggcggct cccggcggtg
61 aggccgcgcc tgtccgggga tcgtcgaggg acggcgggag cttgggccag cggcggcggc
121 ggcctgggac gcaggcggag ccccgcgcag gcccaaggtc ccggaggcta tggcagcggc
181 taccatcgtg cacgacacgt ctgaggccgt ggagctctgc cctgcttacg gcttgtacct
241 gaagcccatc accaagatga ccatcagcgt ggcactcccg cagctgaagc agccggggaa
301 gtccatctcc aactgggagg tgatggagag gctgaagggc atggtgcaga accaccagtt
361 ctccacgctg cgtatttcca agagcaccat ggacttcatc cgcttcgagg gggaggtgga
421 gaacaagagc ctggtcaagt cttttctggc ctgcctggac ggcaagacca tcaagctcag
481 cggcttctcc gacatcctga aggtgcgcgc ggccgagttc aagatcgact tccccacccg
541 ccacgactgg gactccttct tccgcgacgc caaggacatg aacgagaccc tgccggggga
601 gcggccggac accatccacc tggaggggct gccctgcaag tggttcgccc tgaaggagtc
661 gggctccgag aagcccagcg aggacgtcct ggtcaaggtg tttgagaagt tcggggagat
721 ccggaatgtg gacatcccca tgctggaccc ctaccgggag gagatgacgg gccgcaactt
781 ccacaccttc agtttcgggg ggcacttgaa cttcgaggcc tatgtgcagt accgtgagta
841 catgggcttc atccaggcca tgagcgccct gcgcgggatg aaactcatgt acaagggcga
901 ggacggcaag gccgtggcct gcaacatcaa ggtttctttt gattcgacca aacacctgag
961 tgatgcctca attaagaagc ggcagctgga gaggcagaag cttcaggaac tggagcagca
1021 aagagaagaa caaaagcgca gagagaagga agcggaggag aggcagcgag cggaggaaag
1081 gaaacaaaag gagctggaag agctggagcg agagaggaaa agagaagaga agcttcgcaa
1141 gagggagcag aagcagaggg accgtgagct gcgccggaat cagaagaagc tggagaagct
1201 gcaggcggag gagcagaagc agctgcagga gaagatcaag ctggaggagc gcaagctgct
1261 gctggcccag aggaacctgc agtccatccg gctcatcgcc gagctgctca gcagagccaa
1321 ggctgtgaag ctacgggaac aggagcagaa ggaggagaag ctgaggctcc agcagcagga
1381 ggagcggcgg cggctgcagg aggccgagct gcggcgcgtg gaggaggaga aggagcgcgc
1441 gctgggcctg cagcggaaag agcgggagct gcgcgagcgg ctgctgagca tcctgcagag
1501 caagaagccg gacgacagcc acacacacga cgagctgggc gtggcacacg gacctgctgc
1561 agcccgtcct ggacatcctg cagaccgtgt cgtccggctg tgtgagcgcc accacgctgc
1621 accccctcgg gggccagccc ccggccggtg cccccaagga gagcccggcc cccccagagg
1681 ccgacggcgc tcccaaaagc gtgaacggga gcgtggccga ggaggcccca tgcaaggagg
1741 ttcagagctc ctgtcgtgtg gtccccgagg atggctctcc agagaagagg tgcccgggcg
1801 gcgtcctctc ctgcattcct gacaacaacc aacagcccaa gggcatccct gcctgcgagc
1861 agaatgtctc cagaaaggac acccggtcag aacaggacaa gtgcaaccgg gagcccagca
1921 agggccgggg ccgggccacc ggagacgggc ttgctgaccg gcacaagcgg gagaggagcc
1981 gggccaggcg ggccagcagc agggaggacg ggaggccacg caaggagcgg cggccccaca
2041 agaagcacgc ctacaaggat gacagccccc gccggcgcag cacgagcccg gaccacaccc
2101 ggtcccggag gtcccacagc aaagacaggc accggaggga gcggagccgg gagcggaggg
2161 gcagcgccag caggaagcac agccgccacc gccgccgaag cgagcggtcg cgctcccggt
2221 ccccgagcag gcaccgcagt acctggaaca ggtaatgacg ggcacggcct ccccacggcc
2281 tgtccgggaa agaccaggac ctgctcgagc ctcctggccg ctccttggcc gctctccgtc
2341 cacccctgca aagccaagac ccttctgcag ccacgaatgt ccacggagcc cgccggcagg
2401 aaggaagaca ccatgcttta gagatccatc tttctccact caccgcagcg tacttggcac
2461 ttcagtttca aacacgtagt cctttaaaac ttgatccgat agctttaatg cggccggtcc
2521 tctctcagtc aggaaaattg cacagaccga cagtcgtgag gatggcagag ctgctgcatt
2581 cccccacacg gggatttctg tgtctgcttg gcgacctcct ac
//