LOCUS HUMBRAF 2513 bp mRNA linear HUM 29-JAN-2004
DEFINITION Homo sapiens B-raf protein (BRAF) mRNA, complete cds.
ACCESSION M95712 M95720 X54072
VERSION M95712.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (sites)
AUTHORS Sithanandam,G.
JOURNAL Unpublished
REFERENCE 2 (bases 288 to 2513)
AUTHORS Sithanandam,G., Kolch,W., Duh,F.M. and Rapp,U.R.
TITLE Complete coding sequence of a human B-raf cDNA and detection of
B-raf protein kinase with isozyme specific antibodies
JOURNAL Oncogene 5 (12), 1775-1780 (1990)
PUBMED 2284096
REFERENCE 3 (sites)
AUTHORS Stephens,R.M., Sithanandam,G., Copeland,T., Kaplan,D.R., Rapp,U.R.
and Morrison,D.K.
TITLE 95kDa b-Raf serine/threonine kinase: idendification of the protein
and its major autophosphorylation site
JOURNAL Unpublished
REFERENCE 4 (bases 1 to 2513)
AUTHORS Sithanandam,G., Kolch,W., Duh,F.M. and Rapp,U.R.
TITLE Direct Submission
JOURNAL Submitted (27-APR-1993) Laboratory of Viral Carcinogenesis,
National Cancer Institute, Frederick Cancer Research and
Development Center, Frederick, MD, USA
REFERENCE 5 (bases 1 to 2513)
AUTHORS Albert,S., Wixler,L. and Rapp,U.R.
TITLE Direct Submission
JOURNAL Submitted (29-JAN-2004) Institute of Medical Radiation and Cell
Research, Versbacherstr. 5, Wuerzburg 97078, Germany
REMARK Sequence update by submitter
COMMENT On Jan 29, 2004 this sequence version replaced M95712.1.
Original source text: Homo sapiens RNA.
From EMBL 27 entry HSBRAF; dated 11-MAR-1991.
FEATURES Location/Qualifiers
source 1..2513
/db_xref="H-InvDB:HIT000196904"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="testes"
gene 1..2513
/gene="BRAF"
5'UTR 1..61
/gene="BRAF"
/note="putative"
CDS 62..2362
/gene="BRAF"
/note="serine/threonine protein kinase; b-raf oncogene"
/codon_start=1
/product="B-raf protein"
/protein_id="AAA35609.2"
/translation="MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIP
EEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLES
LGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVF
LPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLT
GEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEV
PLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSP
SPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDG
GSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDW
EIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKT
RHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDY
LHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEV
IRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVR
SNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQT
EDFSLYACASPKTPIQAGGYGAFPVH"
regulatory 2406..2411
/regulatory_class="polyA_signal_sequence"
/gene="BRAF"
regulatory 2442..2447
/regulatory_class="polyA_signal_sequence"
/gene="BRAF"
BASE COUNT 747 a 579 c 566 g 621 t
ORIGIN
1 cgcctcccgg ccccctcccc gcccgacagc ggccgctcgg gccccggctc tcggttataa
61 gatggcggcg ctgagcggtg gcggtggtgg cggcgcggag ccgggccagg ctctgttcaa
121 cggggacatg gagcccgagg ccggcgccgg cgccggcgcc gcggcctctt cggctgcgga
181 ccctgccatt ccggaggagg tgtggaatat caaacaaatg attaagttga cacaggaaca
241 tatagaggcc ctattggaca aatttggtgg ggagcataat ccaccatcaa tatatctgga
301 ggcctatgaa gaatacacca gcaagctaga tgcactccaa caaagagaac aacagttatt
361 ggaatctctg gggaacggaa ctgatttttc tgtttctagc tctgcatcaa tggataccgt
421 tacatcttct tcctcttcta gcctttcagt gctaccttca tctctttcag tttttcaaaa
481 tcccacagat gtggcacgga gcaaccccaa gtcaccacaa aaacctatcg ttagagtctt
541 cctgcccaac aaacagagga cagtggtacc tgcaaggtgt ggagttacag tccgagacag
601 tctaaagaaa gcactgatga tgagaggtct aatcccagag tgctgtgctg tttacagaat
661 tcaggatgga gagaagaaac caattggttg ggacactgat atttcctggc ttactggaga
721 agaattgcat gtggaagtgt tggagaatgt tccacttaca acacacaact ttgtacgaaa
781 aacgtttttc accttagcat tttgtgactt ttgtcgaaag ctgcttttcc agggtttccg
841 ctgtcaaaca tgtggttata aatttcacca gcgttgtagt acagaagttc cactgatgtg
901 tgttaattat gaccaacttg atttgctgtt tgtctccaag ttctttgaac accacccaat
961 accacaggaa gaggcgtcct tagcagagac tgccctaaca tctggatcat ccccttccgc
1021 acccgcctcg gactctattg ggccccaaat tctcaccagt ccgtctcctt caaaatccat
1081 tccaattcca cagcccttcc gaccagcaga tgaagatcat cgaaatcaat ttgggcaacg
1141 agaccgatcc tcatcagctc ccaatgtgca tataaacaca atagaacctg tcaatattga
1201 tgacttgatt agagaccaag gatttcgtgg tgatggagga tcaaccacag gtttgtctgc
1261 taccccccct gcctcattac ctggctcact aactaacgtg aaagccttac agaaatctcc
1321 aggacctcag cgagaaagga agtcatcttc atcctcagaa gacaggaatc gaatgaaaac
1381 acttggtaga cgggactcga gtgatgattg ggagattcct gatgggcaga ttacagtggg
1441 acaaagaatt ggatctggat catttggaac agtctacaag ggaaagtggc atggtgatgt
1501 ggcagtgaaa atgttgaatg tgacagcacc tacacctcag cagttacaag ccttcaaaaa
1561 tgaagtagga gtactcagga aaacacgaca tgtgaatatc ctactcttca tgggctattc
1621 cacaaagcca caactggcta ttgttaccca gtggtgtgag ggctccagct tgtatcacca
1681 tctccatatc attgagacca aatttgagat gatcaaactt atagatattg cacgacagac
1741 tgcacagggc atggattact tacacgccaa gtcaatcatc cacagagacc tcaagagtaa
1801 taatatattt cttcatgaag acctcacagt aaaaataggt gattttggtc tagctacagt
1861 gaaatctcga tggagtgggt cccatcagtt tgaacagttg tctggatcca ttttgtggat
1921 ggcaccagaa gtcatcagaa tgcaagataa aaatccatac agctttcagt cagatgtata
1981 tgcatttggg attgttctgt atgaattgat gactggacag ttaccttatt caaacatcaa
2041 caacagggac cagataattt ttatggtggg acgaggatac ctgtctccag atctcagtaa
2101 ggtacggagt aactgtccaa aagccatgaa gagattaatg gcagagtgcc tcaaaaagaa
2161 aagagatgag agaccactct ttccccaaat tctcgcctct attgagctgc tggcccgctc
2221 attgccaaaa attcaccgca gtgcatcaga accctccttg aatcgggctg gtttccaaac
2281 agaggatttt agtctatatg cttgtgcttc tccaaaaaca cccatccagg cagggggata
2341 tggtgcgttt cctgtccact gaaacaaatg agtgagagag ttcaggagag tagcaacaaa
2401 aggaaaataa atgaacatat gtttgcttat atgttaaatt gaataaaata ctctcttttt
2461 ttttaaggtg gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ccc
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