LOCUS HUMNF1B 9026 bp mRNA linear HUM 07-JAN-1995
DEFINITION Human neurofibromin (NF1) gene, complete cds.
ACCESSION M89914
VERSION M89914.1
KEYWORDS neurofibromatosis protein type 1; neurofibromin; tumor suppressor.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (sites)
AUTHORS Xu,G., O'Connell,P., Viskochil,D., Cawthon,R., Robertson,M.,
Culver,M., Dunn,D., Stevens,J., Gesteland,R., White,R. and
Weiss,R.L.
TITLE The neurofibromatosis type 1 gene encodes a protein related to GAP
JOURNAL Cell 62 (3), 599-608 (1990)
PUBMED 2116237
REFERENCE 2 (sites)
AUTHORS Marchuk,D.A., Saulino,A.M., Tavakkol,R., Swaroop,M., Wallace,M.R.,
Andersen,L.B., Mitchell,A.L., Gutmann,D.H., Boguski,M. and
Collins,F.S.
TITLE cDNA cloning of the type 1 neurofibromatosis gene: complete
sequence of the NF1 gene product
JOURNAL Genomics 11 (4), 931-940 (1991)
PUBMED 1783401
REFERENCE 3 (sites)
AUTHORS Nishi,T., Lee,P.S., Oka,K., Levin,V.A., Tanase,S., Morino,Y. and
Saya,H.
TITLE Differential expression of two types of the neurofibromatosis type
1 (NF1) gene transcripts related to neuronal differentiation
JOURNAL Oncogene 6 (9), 1555-1559 (1991)
PUBMED 1923522
REFERENCE 4 (bases 1 to 9026)
AUTHORS Bernards,A., Haase,V.H., Murthy,A.E., Menon,A., Hannigan,G.E. and
Gusella,J.F.
TITLE Complete human NF1 cDNA sequence: two alternatively spliced mRNAs
and absence of expression in a neuroblastoma line
JOURNAL DNA Cell Biol. 11 (10), 727-734 (1992)
PUBMED 1457041
COMMENT On Jun 12, 1993 this sequence version replaced gi:189166.
Original source text: Homo sapiens cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..9026
/db_xref="H-InvDB:HIT000196759"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="Nalm6 pre-B cell and RIC2 retinal cell"
/germline
gene 1..9026
/gene="NF1"
CDS 198..8717
/gene="NF1"
/codon_start=1
/product="neurofibromin"
/protein_id="AAA59925.1"
/translation="MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLI
NISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTM
RLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTR
LQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEK
AFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLIL
CPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYI
NWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQ
HFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKA
VQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLL
CNPRKQGPETQGSTAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAP
VETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHF
LLFYGVGCDIPSSGNTSQMSMDHEELLRTPGASLRKGKGNSSMDSAAGCSGTPPICRQ
AQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNLLPNYNTFME
FASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTHAKWEQATKLILNYPKAK
MEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRS
NSGLATYSPPMGPVSERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIR
TNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNLL
DNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLS
FCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQASMEAVVSLLAGLPLQPE
EGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDESAQTGGRKRGMSRRLASLRHCTV
LAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADR
FERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFS
KEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFE
VDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLN
KATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIE
RGLKLMSKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDAVNH
SLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHK
PVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVA
RRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPG
FAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLVFIDCPGKLAEHIEHEQQKL
PAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYY
ASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQH
TKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCI
PANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVR
FCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIK
TSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLM
WDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHS
LCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPG
SYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRA
LVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIEATVIALTKLQPLLN
KDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIR
NPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNK
HRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHH
GDPSYRTLKETQPWSSPKGSEGYLAATYPTVGQTSPRARKSMSLDMGQPSQANTKKLL
GTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPHLRKV
SVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVF
PVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLW
RFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQL
SITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAG
SFKRNSIKKIV"
exon 4309..4371
/gene="NF1"
/note="alternative"
BASE COUNT 2635 a 2026 c 1911 g 2454 t
ORIGIN
1 ccggggcggc gcgggggcct cccactcccg ctcggcgctg accccccatc cccacccccg
61 tgggaacact gggagcctgc actccacaga ccctctcctt gcctcttccc tcacctcagc
121 ctccgctccc cgccctcttc ccggcccagg gcgccggccc acccttccct ccgccgcccc
181 ccggccgcgg ggaggacatg gccgcgcaca ggccggtgga atgggtccag gccgtggtca
241 gccgcttcga cgagcagctt ccaataaaaa caggacagca gaacacacat accaaagtca
301 gtactgagca caacaaggaa tgtctaatca atatttccaa atacaagttt tctttggtta
361 taagcggcct cactactatt ttaaagaatg ttaacaatat gagaatattt ggagaagctg
421 ctgaaaaaaa tttatatctc tctcagttga ttatattgga tacactggaa aaatgtcttg
481 ctgggcaacc aaaggacaca atgagattag atgaaacgat gctggtcaaa cagttgctgc
541 cagaaatctg ccattttctt cacacctgtc gtgaaggaaa ccagcatgca gctgaacttc
601 ggaattctgc ctctggggtt ttattttctc tcagctgcaa caacttcaat gcagtcttta
661 gtcgcatttc taccaggtta caggaattaa ctgtttgttc agaagacaat gttgatgttc
721 atgatataga attgttacag tatatcaatg tggattgtgc aaaattaaaa cgactcctga
781 aggaaacagc atttaaattt aaagccctaa agaaggttgc gcagttagca gttataaata
841 gcctggaaaa ggcattttgg aactgggtag aaaattatcc agatgaattt acaaaactat
901 accagatccc acagactgat atggctgaat gtgcagaaaa gctatttgac ttggtggatg
961 gttttgctga aagcaccaaa cgtaaagcag cagtttggcc actacaaatc attctcctta
1021 tcttgtgtcc agaaataatc caggatatat ccaaagacgt ggttgatgaa aacaacatga
1081 ataagaagtt atttctggac agtctacgaa aagctcttgc tggccatgga ggaagtaggc
1141 agctgacaga aagtgctgca attgcctgtg tcaaactgtg taaagcaagt acttacatca
1201 attgggaaga taactctgtc attttcctac ttgttcagtc catggtggtt gatcttaaga
1261 acctgctttt taatccaagt aagccattct caagaggcag tcagcctgca gatgtggatc
1321 taatgattga ctgccttgtt tcttgctttc gtataagccc tcacaacaac caacacttta
1381 agatctgcct ggctcagaat tcaccttcta catttcacta tgtgctggta aattcactcc
1441 atcgaatcat caccaattcc gcattggatt ggtggcctaa gattgatgct gtgtattgtc
1501 actcggttga acttcgaaat atgtttggtg aaacacttca taaagcagtg caaggttgtg
1561 gagcacaccc agcaatacga atggcaccga gtcttacatt taaagaaaaa gtaacaagcc
1621 ttaaatttaa agaaaaacct acagacctgg agacaagaag ctataagtat cttctcttgt
1681 ccatggtgaa actaattcat gcagatccaa agctcttgct ttgtaatcca agaaaacagg
1741 ggcccgaaac ccaaggcagt acagcagaat taattacagg gctcgtccaa ctggtccctc
1801 agtcacacat gccagagatt gctcaggaag caatggaggc tctgctggtt cttcatcagt
1861 tagatagcat tgatttgtgg aatcctgatg ctcctgtaga aacattttgg gagattagct
1921 cacaaatgct tttttacatc tgcaagaaat taactagtca tcaaatgctt agtagcacag
1981 aaattctcaa gtggttgcgg gaaatattga tctgcaggaa taaatttctt cttaaaaata
2041 agcaggcaga tagaagttcc tgtcactttc tcctttttta cggggtagga tgtgatattc
2101 cttctagtgg aaataccagt caaatgtcca tggatcatga agaattacta cgtactcctg
2161 gagcctctct ccggaaggga aaagggaact cctctatgga tagtgcagca ggatgcagcg
2221 gaaccccccc gatttgccga caagcccaga ccaaactaga agtggccctg tacatgtttc
2281 tgtggaaccc tgacactgaa gctgttctgg ttgccatgtc ctgtttccgc cacctctgtg
2341 aggaagcaga tatccggtgt ggggtggatg aagtgtcagt gcataacctc ttgcccaact
2401 ataacacatt catggagttt gcctctgtca gcaatatgat gtcaacagga agagcagcac
2461 ttcagaaaag agtgatggca ctgctgaggc gcattgagca tcccactgca ggaaacactg
2521 aggcttggga agatacacat gcaaaatggg aacaagcaac aaagctaatc cttaactatc
2581 caaaagccaa aatggaagat ggccaggctg ctgaaagcct tcacaagacc attgttaaga
2641 ggcgaatgtc ccatgtgagt ggaggaggat ccatagattt gtctgacaca gactccctac
2701 aggaatggat caacatgact ggcttccttt gtgcccttgg gggagtgtgc ctccagcaga
2761 gaagcaattc tggcctggca acctatagcc cacccatggg tccagtcagt gaacgtaagg
2821 gttctatgat ttcagtgatg tcttcagagg gaaacgcaga tacacctgtc agcaaattta
2881 tggatcggct gttgtcctta atggtgtgta accatgagaa agtgggactt caaatacgga
2941 ccaatgttaa ggatctggtg ggtctagaat tgagtcctgc tctgtatcca atgctattta
3001 acaaattgaa gaataccatc agcaagtttt ttgactccca aggacaggtt ttattgactg
3061 ataccaatac tcaatttgta gaacaaacca tagctataat gaagaacttg ctagataatc
3121 atactgaagg cagctctgaa catctagggc aagctagcat tgaaacaatg atgttaaatc
3181 tggtcaggta tgttcgtgtg cttgggaata tggtccatgc aattcaaata aaaacgaaac
3241 tgtgtcaatt agttgaagta atgatggcaa ggagagatga cctctcattt tgccaagaga
3301 tgaaatttag gaataagatg gtagaatacc tgacagactg ggttatggga acatcaaacc
3361 aagcagcaga tgatgatgta aaatgtctta caagagattt ggaccaggca agcatggaag
3421 cagtagtttc acttctagct ggtctccctc tgcagcctga agaaggagat ggtgtggaat
3481 tgatggaagc caaatcacag ttatttctta aatacttcac attatttatg aaccttttga
3541 atgactgcag tgaagttgaa gatgaaagtg cgcaaacagg tggcaggaaa cgtggcatgt
3601 ctcggaggct ggcatcactg aggcactgta cggtccttgc aatgtcaaac ttactcaatg
3661 ccaacgtaga cagtggtctc atgcactcca taggcttagg ttaccacaag gatctccaga
3721 caagagctac atttatggaa gttctgacaa aaatccttca acaaggcaca gaatttgaca
3781 cacttgcaga aacagtattg gctgatcggt ttgagagatt ggtggaactg gtcacaatga
3841 tgggtgatca aggagaactc cctatagcga tggctctggc caatgtggtt ccttgttctc
3901 agtgggatga actagctcga gttctggtta ctctgtttga ttctcggcat ttactctacc
3961 aactgctctg gaacatgttt tctaaagaag tagaattggc agactccatg cagactctct
4021 tccgaggcaa cagcttggcc agtaaaataa tgacattctg tttcaaggta tatggtgcta
4081 cctatctaca aaaactcctg gatcctttat tacgaattgt gatcacatcc tctgattggc
4141 aacatgttag ctttgaagtg gatcctacca ggttagaacc atcagagagc cttgaggaaa
4201 accagcggaa cctccttcag atgactgaaa agttcttcca tgccatcatc agttcctcct
4261 cagaattccc ccctcaactt cgaagtgtgt gccactgttt ataccaggca acttgccact
4321 ccctactgaa taaagctaca gtaaaagaaa aaaaggaaaa caaaaaatca gtggttagcc
4381 agcgtttccc tcagaacagc atcggtgcag taggaagtgc catgttcctc agatttatca
4441 atcctgccat tgtctcaccg tatgaagcag ggattttaga taaaaagcca ccacctagaa
4501 tcgaaagggg cttgaagtta atgtcaaaga tacttcagag tattgccaat catgttctct
4561 tcacaaaaga agaacatatg cggcctttca atgattttgt gaaaagcaac tttgatgcag
4621 cacgcaggtt tttccttgat atagcatctg attgtcctac aagtgatgca gtaaatcata
4681 gtctttcctt cataagtgac ggcaatgtgc ttgctttaca tcgtctactc tggaacaatc
4741 aggagaaaat tgggcagtat ctttccagca acagggatca taaagctgtt ggaagacgac
4801 cttttgataa gatggcaaca cttcttgcat acctgggtcc tccagagcac aaacctgtgg
4861 cagatacaca ctggtccagc cttaacctta ccagttcaaa gtttgaggaa tttatgacta
4921 ggcatcaggt acatgaaaaa gaagaattca aggctttgaa aacgttaagt attttctacc
4981 aagctgggac ttccaaagct gggaatccta ttttttatta tgttgcacgg aggttcaaaa
5041 ctggtcaaat caatggtgat ttgctgatat accatgtctt actgacttta aagccatatt
5101 atgcaaagcc atatgaaatt gtagtggacc ttacccatac cgggcctagc aatcgcttta
5161 aaacagactt tctctctaag tggtttgttg tttttcctgg ctttgcttac gacaacgtct
5221 ccgcagtcta tatctataac tgtaactcct gggtcaggga gtacaccaag tatcatgagc
5281 ggctgctgac tggcctcaaa ggtagcaaaa ggcttgtttt catagactgt cctgggaaac
5341 tggctgagca catagagcat gaacaacaga aactacctgc tgccaccttg gctttagaag
5401 aggacctgaa ggtattccac aatgctctca agctagctca caaagacacc aaagtttcca
5461 ttaaagttgg ttctactgct gtccaagtaa cttcagcaga gcgaacaaaa gtcctagggc
5521 aatcagtctt tctaaatgac atttattatg cttcggaaat tgaagaaatc tgcctagtag
5581 atgagaacca gttcacctta accattgcaa accagggcac gccgctcacc ttcatgcacc
5641 aggagtgtga agccattgtc cagtctatca ttcatatccg gacccgctgg gaactgtcac
5701 agcccgactc tatcccccaa cacaccaaga ttcggccaaa agatgtccct gggacactgc
5761 tcaatatcgc attacttaat ttaggcagtt ctgacccgag tttacggtca gctgcctata
5821 atcttctgtg tgccttaact tgtaccttta atttaaaaat cgagggccag ttactagaga
5881 catcaggttt atgtatccct gccaacaaca ccctctttat tgtctctatt agtaagacac
5941 tggcagccaa tgagccacac ctcacgttag aatttttgga agagtgtatt tctggattta
6001 gcaaatctag tattgaattg aaacaccttt gtttggaata catgactcca tggctgtcaa
6061 atctagttcg tttttgcaag cataatgatg atgccaaacg acaaagagtt actgctattc
6121 ttgacaagct gataacaatg accatcaatg aaaaacagat gtacccatct attcaagcaa
6181 aaatatgggg aagccttggg cagattacag atctgcttga tgttgtacta gacagtttca
6241 tcaaaaccag tgcaacaggt ggcttgggat caataaaagc tgaggtgatg gcagatactg
6301 ctgtagcttt ggcttctgga aatgtgaaat tggtttcaag caaggttatt ggaaggatgt
6361 gcaaaataat tgacaagaca tgcttatctc caactcctac tttagaacaa catcttatgt
6421 gggatgatat tgctatttta gcacgctaca tgctgatgct gtccttcaac aattcccttg
6481 atgtggcagc tcatcttccc tacctcttcc acgttgttac tttcttagta gccacaggtc
6541 cgctctccct tagagcttcc acacatggac tggtcattaa tatcattcac tctctgtgta
6601 cttgttcaca gcttcatttt agtgaagaga ccaagcaagt tttgagactc agtctgacag
6661 agttctcatt acccaaattt tacttgctgt ttggcattag caaagtcaag tcagctgctg
6721 tcattgcctt ccgttccagt taccgggaca ggtcattctc tcctggctcc tatgagagag
6781 agacttttgc tttgacatcc ttggaaacag tcacagaagc tttgttggag atcatggagg
6841 catgcatgag agatattcca acgtgcaagt ggctggacca gtggacagaa ctagctcaaa
6901 gatttgcatt ccaatataat ccatccctgc aaccaagagc tcttgttgtc tttgggtgta
6961 ttagcaaacg agtgtctcat gggcagataa agcagataat ccgtattctt agcaaggcac
7021 ttgagagttg cttaaaagga cctgacactt acaacagtca agttctgata gaagctacag
7081 taatagcact aaccaaatta cagccacttc ttaataagga ctcgcctctg cacaaagccc
7141 tcttttgggt agctgtggct gtgctgcagc ttgatgaggt caacttgtat tcagcaggta
7201 ccgcacttct tgaacaaaac ctgcatactt tagatagtct ccgtatattc aatgacaaga
7261 gtccagagga agtatttatg gcaatccgga atcctctgga gtggcactgc aagcaaatgg
7321 atcattttgt tggactcaat ttcaactcta actttaactt tgcattggtt ggacaccttt
7381 taaaagggta caggcatcct tcacctgcta ttgttgcaag aacagtcaga attttacata
7441 cactactaac tctggttaac aaacacagaa attgtgacaa atttgaagtg aatacacaga
7501 gcgtggccta cttagcagct ttacttacag tgtctgaaga agttcgaagt cgctgcagcc
7561 taaaacatag aaagtcactt cttcttactg atatttcaat ggaaaatgtt cctatggata
7621 catatcccat tcatcatggt gacccttcct ataggacact aaaggagact cagccatggt
7681 cctctcccaa aggttctgaa ggataccttg cagccaccta tccaactgtc ggccagacca
7741 gtccccgagc caggaaatcc atgagcctgg acatggggca accttctcag gccaacacta
7801 agaagttgct tggaacaagg aaaagttttg atcacttgat atcagacaca aaggctccta
7861 aaaggcaaga aatggaatca gggatcacaa caccccccaa aatgaggaga gtagcagaaa
7921 ctgattatga aatggaaact cagaggattt cctcatcaca acagcaccca catttacgta
7981 aagtttcagt gtctgaatca aatgttctct tggatgaaga agtacttact gatccgaaga
8041 tccaggcgct gcttcttact gttctagcta cactggtaaa atataccaca gatgagtttg
8101 atcaacgaat tctttatgaa tacttagcag aggccagtgt tgtgtttccc aaagtctttc
8161 ctgttgtgca taatttgttg gactctaaga tcaacaccct gttatcattg tgccaagatc
8221 caaatttgtt aaatccaatc catggaattg tgcagagtgt ggtgtaccat gaagaatccc
8281 caccacaata ccaaacatct tacctgcaaa gttttggttt taatggcttg tggcggtttg
8341 caggaccgtt ttcaaagcaa acacaaattc cagactatgc tgagcttatt gttaagtttc
8401 ttgatgcctt gattgacacg tacctgcctg gaattgatga agaaaccagt gaagaatccc
8461 tcctgactcc cacatctcct taccctcctg cactgcagag ccagcttagt atcactgcca
8521 accttaacct ttctaattcc atgacctcac ttgcaacttc ccagcattcc ccaggaatcg
8581 acaaggagaa cgttgaactc tcccctacca ctggccactg taacagtgga cgaactcgcc
8641 acggatccgc aagccaagtg cagaagcaaa gaagcgctgg cagtttcaaa cgtaatagca
8701 ttaagaagat cgtgtgaagc ttgcttgctt tcttttttaa aatcaactta acatgggctc
8761 ttcactagtg accccttccc tgtccttgcc ctttcccccc atgttgtaat gctgcacttc
8821 ctgttttata atgaacccat ccggtttgcc atgttgccag atgatcaact cttcgaagcc
8881 ttgcctaaat ttaatgctgc cttttcttta acttttttct tctacttttg gcgtgtatct
8941 ggtatatgta agtgttcaga acaactgcaa agaaagtggg aggtcaggaa acttttaact
9001 gagaaatctc aattgtaaga gaggat
//