LOCUS HUMSAP1A 1933 bp mRNA linear HUM 17-DEC-1993
DEFINITION Homo sapiens SRF accessory protein 1A (SAP-1) mRNA, complete cds.
ACCESSION M85165
VERSION M85165.1
KEYWORDS serum response factor; SAP-1; Elk-1.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1933)
AUTHORS Dalton,S. and Treisman,R.
TITLE Characterization of SAP-1, a protein recruited by serum response
factor to the c-fos serum response element
JOURNAL Cell 68 (3), 597-612 (1992)
PUBMED 1339307
REFERENCE 2 (bases 1 to 1933)
AUTHORS Treisman,R.
TITLE Direct Submission
JOURNAL Submitted (05-MAR-1992) Richard Treisman, Transcription Laboratory,
Imperial Cancer Research Fund, London, England
COMMENT On Nov 29, 1993 this sequence version replaced gi:338036.
Original source text: Homo sapiens cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..1933
/db_xref="H-InvDB:HIT000196607"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
gene 1..1933
/gene="SAP-1"
CDS 150..1445
/gene="SAP-1"
/note="Homology region A with Elk-1 protein (Ets domain)
is bp 150-417, amino acids 1-89; Homology region B with
Elk-1 protein, required for cooperative ternary complex
formation with SRF is bp 565-617, amino acids 136-157;
Sequence diverges from SAP-1B at bp 1229, amino acid 360;
Homology region C with Elk-1 protein, core of regulated
transcription activation domain, is bp 1203-1355, amino
acids 352-402; (S/T)P motifs conserved between SAP-1A and
Elk-1 are located at amino acids T354, T361, T366, S381,
S387, T420, S425, corresponding to bp 1209-1214, bp
1230-1235, bp 1245-1250, bp 1290-1295, bp 1308-1313, bp
1407-1412, bp-1422-1427"
/codon_start=1
/product="SAP-1A protein"
/protein_id="AAA03631.1"
/translation="MDSAITLWQFLLQLLQKPQNKHMICWTSNDGQFKLLQAEEVARL
WGIRKNKPNMNYDKLSRALRYYYVKNIIKKVNGQKFVYKFVSYPEILNMDPMTVGRIE
GDCESLNFSEVSSSSKDVENGGKDKPPQPGAKTSSRNDYIHSGLYSSFTLNSLNSSNV
KLFKLIKTENPAEKLAEKKSPQEPTPSVIKFVTTPSKKPPVEPVAATISIGPSISPSS
EETIQALETLVSPKLPSLEAPTSASNVMTAFATTPPISSIPPLQEPPRTPSPPLSSHP
DIDTDIDSVASQPMELPENLSLEPKDQDSVLLEKDKVNNSSRSKKPKGLGLAPTLVIT
SSDPSPLGILSPSLPTASLTPAFFSQTPIILTPSPLLSSIHFWSTLSPVAPLSPARLQ
GANTLFQFPSVLNSHGPFTLSGLDGPSTPGPFSPDLQKT"
BASE COUNT 556 a 486 c 385 g 506 t
ORIGIN
1 ccgccgcctt ctactccgcc gcgggggtcg cagcggctgc cgcgccgtcc tcgagtttcc
61 agcgtgagga ggaggctgag ggcggagagg cgcatcgtgt tcgaggcgga gaccgagggg
121 gagccccgcg cgcggcgtcg ctcattgcta tggacagtgc tatcaccctg tggcagttcc
181 ttcttcagct cctgcagaag cctcagaaca agcacatgat ctgttggacc tctaatgatg
241 ggcagtttaa gcttttgcag gcagaagagg tggctcgtct ctgggggatt cgcaagaaca
301 agcctaacat gaattatgac aaactcagcc gagccctcag atactattat gtaaagaata
361 tcatcaaaaa agtgaatggt cagaagtttg tgtacaagtt tgtctcttat ccagagattt
421 tgaacatgga tccaatgaca gtgggcagga ttgagggtga ctgtgaaagt ttaaacttca
481 gtgaagtcag cagcagttcc aaagatgtgg agaatggagg gaaagataaa ccacctcagc
541 ctggtgccaa gacctctagc cgcaatgact acatacactc tggcttatat tcttcattta
601 ctctcaactc tttgaactcc tccaatgtaa agcttttcaa attgataaag actgagaatc
661 cagccgagaa actggcagag aaaaaatctc ctcaggagcc cacaccatct gtcatcaaat
721 ttgtcacgac accttccaaa aagccaccag ttgaacctgt tgctgccacc atttcaattg
781 gcccaagtat ttctccatct tcagaagaaa ctatccaagc tttggagaca ttggtttccc
841 caaaactgcc ttccctggaa gccccaacct ctgcctctaa cgtaatgact gcttttgcca
901 ccacaccacc catttcgtcc ataccccctt tgcaggaacc tcccagaaca ccttcaccac
961 cactgagttc tcacccagac atcgacacag acattgattc agtggcttct cagccaatgg
1021 aacttccaga gaatttgtct ctggagccta aagaccagga ttcagtcttg ctagaaaagg
1081 acaaagtaaa taattcatca agatccaaga aacccaaagg gttaggactg gcacccaccc
1141 ttgtgatcac gagcagtgat ccaagcccac tgggaatact gagcccatct ctccctacag
1201 cttctcttac accagcattt ttttcacaga cacccatcat actgactcca agccccttgc
1261 tctccagtat ccacttctgg agtactctca gtcctgttgc tcccctaagt ccagccagac
1321 tgcaaggtgc taacacactt ttccagtttc cttctgtact gaacagtcat gggccattca
1381 ctctgtctgg gctggatgga ccttccaccc ctggcccatt ttccccagac ctacagaaga
1441 cataacctat gcacttgtgg aatgagagaa ccgaggaacg aagaaacaga cattcaacat
1501 gattgcattt gaagtgagca attgatagtt ctacaatgct gataatagac tattgtgatt
1561 tttgccattc cccattgaaa acatcttttt aggattctct ttgaatagga ctcaagttgg
1621 actatatgta taaaaatgcc ttaattggag tctaaactcc acctccctct gtcttttcct
1681 tttctttttc tttccttcct tccttttctt ttctccttta aaaatatttt gagctttgtg
1741 ctgaagaagt ttttggtggg ctttagtgac tgtgctttgc aaaagcaatt aagaacaaag
1801 ttactccttc tggctattgg gaccctttgg ccaggaaaaa ttatgcttag aatctattat
1861 ttaaagaagt atttgtgaaa tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1921 aaaaaaaaaa aaa
//