LOCUS HUMSF2P33 1428 bp mRNA linear HUM 24-NOV-1993 DEFINITION Human SF2p33 mRNA, complete cds. ACCESSION M69040 VERSION M69040.1 KEYWORDS SF2p33. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1428) AUTHORS Krainer,A.R., Mayeda,A., Kozak,D. and Binns,G. TITLE Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators JOURNAL Cell 66 (2), 383-394 (1991) PUBMED 1830244 COMMENT Original source text: Homo sapiens cDNA to mRNA. Comments: SF2p33 is an essential pre-mRNA splicing factor, which can also affect alternative 5' splice site selection in vitro by stimulating the use of proximal 5' splice sites. (Krainer, A.R., Conway, G.C., and Kozak, D. (1990) Purification and Characterization of SF2, a Human Pre-mRNA Splicing Factor. Genes Dev. 4, 1158-1171; Krainer, A.R., Conway, G.C., and Kozak, D. (1990) The Essential Pre-mRNA Splicing Factor SF2 Influences 5' Splice Site Selection by Activating Proximal Sites. Cell 62, 35-42). This factor is also known as ASF (Ge, H., and Manley, J.L. (1990) A protein Factor, ASF, Controls Alternative Splicing of SV40 Early Pre-mRNA In Vitro. Cell 62, 25-34). SF2 p33 consists of two polypeptides of apparent molecular weight 33 kd, approximately, although their predicted molecular weight is 27,744 daltons. The two forms appear to differ by the extent of post-translational modification, which includes phosphorylation. SF2p33 binds RNA and promotes the annealing of complementary RNAs. It is required for assembly of pre-spliceosome complexes. The N-termini of the HeLa polypeptides are blocked. FEATURES Location/Qualifiers source 1..1428 /db_xref="H-InvDB:HIT000196208" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /sex="female" 5'UTR <1..124 CDS 125..871 /function="pre-mRNA splicing factor" /codon_start=1 /product="SF2p33" /protein_id="AAA03476.1" /translation="MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRD IDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGG GGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVV EFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSR SNSRSRSYSPRRSRGSPRYSPRHSRSRSRT" 3'UTR 872..1428 BASE COUNT 341 a 296 c 400 g 391 t ORIGIN 1 cgagctgata aaggcgccat tttggagggg ccgcgggaga cgtggtgccg ctgcgggctc 61 gctctgccgt gcgctaggct tggtgggaag gcctgttctc gagtccgcgc ttttcgtcac 121 cgccatgtcg ggaggtggtg tgattcgtgg ccccgcaggg aacaacgatt gccgcatcta 181 cgtgggtaac ttacctccag acatccgaac caaggacatt gaggacgtgt tctacaaata 241 cggcgctatc cgcgacatcg acctcaagaa tcgccgcggg ggaccgccct tcgccttcgt 301 tgagttcgag gacccgcgag acgcggaaga cgcggtgtat ggtcgcgacg gctatgatta 361 cgatgggtac cgtctgcggg tggagtttcc tcgaagcggc cgtggaacag gccgaggcgg 421 cggcgggggt ggaggtggcg gagctccccg aggtcgctat ggccccccat ccaggcggtc 481 tgaaaacaga gtggttgtct ctggactgcc tccaagtgga agttggcagg atttaaagga 541 tcacatgcgt gaagcaggtg atgtatgtta tgctgatgtt taccgagatg gcactggtgt 601 cgtggagttt gtacggaaag aagatatgac ctatgcagtt cgaaaactgg ataacactaa 661 gtttagatct catgagggag aaactgccta catccgggtt aaagttgatg ggcccagaag 721 tccaagttat ggaagatctc gatctcgaag ccgtagtcgt agcagaagcc gtagcagaag 781 caacagcagg agtcgcagtt actccccaag gagaagcaga ggatcaccac gctattctcc 841 ccgtcatagc agatctcgct ctcgtacata agatgattgg tgacactttt tgtagaaccc 901 atgttgtata cagttttcct ttattcagta caatcttttc attttttaat tcaaactgtt 961 ttgttcagaa tgggctaaag tgttgaattg cattcttgta atatcccctt gctcctaaca 1021 tctacattcc cttcgtgtct ttgataaatt gtattttaag tgatgtcata gacaggattg 1081 tttaaattta gttaactcca tactcttcag actgtgatat tgtgtaaatg tctatctgcc 1141 ctggtttgtg tgaactggga tgttgggggt gtttgtggtt atcttacctg gggaagttct 1201 tatgtttatc ttgcttttca tgtgtctttc tgtagacata tctgaagaga tggattaaga 1261 atgctttgga ttaaggattg tggagcacat ttcaatcatt ttaggattgt caaaaggagg 1321 attgaggagg atcagatcaa taatggaggc aatggtatga ctccaagtgc tattgtcaca 1381 gatgaaattg gcagtattga ccttatacta aaaggcaggg gctaaaaa //