LOCUS HUMAUTOANT 1542 bp mRNA linear HUM 31-DEC-1994
DEFINITION Human 75-kD autoantigen (PM-Sc1) mRNA, complete cds.
ACCESSION M58460
VERSION M58460.1
KEYWORDS autoantigen; nucleolar protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1542)
AUTHORS Alderuccio,F., Chan,E.K. and Tan,E.M.
TITLE Molecular characterization of an autoantigen of PM-Scl in the
polymyositis/scleroderma overlap syndrome: a unique and complete
human cDNA encoding an apparent 75-kD acidic protein of the
nucleolar complex
JOURNAL J. Exp. Med. 173 (4), 941-952 (1991)
PUBMED 2007859
COMMENT Original source text: Homo sapiens (tissue library: lambda gt11)
lymphoblastoma cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..1542
/db_xref="H-InvDB:HIT000195869"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="MOLT-4"
/cell_type="T-cell"
/tissue_type="lymphoblastoma"
/tissue_lib="lambda gt11"
gene 1..1542
/gene="PM-Sc1"
CDS 420..1487
/gene="PM-Sc1"
/note="75-kD"
/codon_start=1
/product="autoantigen"
/protein_id="AAA58384.1"
/translation="MAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEK
VWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLS
IHHMPICVSFAFFQQGTYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLK
DQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAP
IDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLEDSEKEDDEG
GGDQAIILDGIKMDTGVEVSDIGSQDAPIILSDSEEEEMIILEPDKNPKKIRTQTTSA
KQEKAPSKKPVKRRKKKRAAN"
BASE COUNT 494 a 283 c 355 g 410 t
ORIGIN
1 cgaccggcac gttcacccca tccctcaggc tttatttatt ttttttcgac aggttctttt
61 caaggctcca gtcaccgcag cagttgtcca tgctgtagtt tccactttcc tgtatgggcg
121 ggctggttag gattccactt tcccccaagt gcttagccca gggccagaca aaaagtagtt
181 gcttaagaaa tacttgttga aggaataaat taatgaatga atttgtgctt acagcggctg
241 gatggcagac aaacctatga ttataggaac atcaggatct catttggaac agattacgga
301 tgctgcattg tggaacttgg aaaaacaaga gttcttggac aggtttcctg tgaacttgtg
361 tctccaaaac tcaatcgggc aacagaaggt attctttttt taaccttgaa ctctctcaga
421 tggccgctcc agctttcgaa cctggcaggc agtcagatct cttggtgaag ttgaatcgac
481 tcatggaaag atgtctaaga aattcgaagt gtatagacac tgagtctctc tgtgttgttg
541 ctggtgaaaa ggtttggcaa atacgtgtag acctacattt attaaatcat gatggaaata
601 ttattgatgc tgccagcatt gctgcaatcg tggccttatg tcatttccga agacctgatg
661 tctctgtcca aggagatgaa gtaacactgt atacacctga agagcgtgat cctgtaccat
721 taagtatcca ccacatgccc atttgtgtca gttttgcctt tttccagcaa ggaacatatt
781 tattggtgga tcccaatgaa cgagaagaac gtgtgatgga tggcttgctg gtgattgcca
841 tgaacaaaca tcgagagatt tgtactatcc agtccagtgg tgggataatg ctactaaaag
901 atcaagttct gagatgcagt aaaatcgctg gtgtgaaagt agcagaaatt acagagctaa
961 tattgaaagc tttggagaat gaccaaaaag taaggaaaga aggtggaaag tttggttttg
1021 cagagtctat agcaaatcaa aggatcacag catttaaaat ggaaaaggcc cctattgata
1081 cctcggatgt agaagaaaaa gcagaagaaa tcattgctga agcagaacct ccttcagaag
1141 ttgtttctac acctgtgcta tggactcctg gaactgccca aattggagag ggagtagaaa
1201 actcctgggg tgatcttgaa gactctgaga aggaagatga tgaaggcggt ggtgatcaag
1261 ctatcattct tgatggtata aaaatggaca ctggagtaga agtctctgat attggaagcc
1321 aagatgctcc cataatactc tcagatagtg aagaagaaga aatgatcatt ttggaaccag
1381 acaagaatcc aaagaaaata agaacacaga ccaccagtgc aaaacaagaa aaagcaccaa
1441 gtaaaaagcc agtgaaaaga agaaaaaaga agagagctgc caattaaagc taacagttgt
1501 atatctgtat atataactat taaaagggat atttattcca tt
//