LOCUS HUMSAPABCD 2171 bp mRNA linear HUM 09-JAN-1995
DEFINITION Human saposin proteins A-D mRNA, complete cds.
ACCESSION M32221
VERSION M32221.1
KEYWORDS saposin.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2171)
AUTHORS Kretz,K.A., Carson,G.S., Morimoto,S., Kishimoto,Y., Fluharty,A.L.
and O'Brien,J.S.
TITLE Characterization of a mutation in a family with saposin B
deficiency: a glycosylation site defect
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87 (7), 2541-2544 (1990)
PUBMED 2320574
COMMENT Original source text: Human lymphoblast, cDNA to mRNA.
Draft entry and computer-readable sequence for [1] kindly submitted
by J.S.O'Brien, 22-MAR-1990.
FEATURES Location/Qualifiers
source 1..2171
/db_xref="H-InvDB:HIT000195459"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="10q21-q22"
gene 1..2171
/gene="PSAP"
CDS 7..1581
/gene="PSAP"
/note="prosaposin"
/codon_start=1
/protein_id="AAA60303.1"
/db_xref="GDB:G00-120-366"
/translation="MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGA
VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF
VQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKE
MPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKT
EKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTR
LPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQ
CDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTET
AAQCNAVEHCKRHVWN"
mat_peptide 184..435
/gene="PSAP"
/product="saposin A"
mat_peptide 589..831
/gene="PSAP"
/product="saposin B"
mat_peptide 937..1176
/gene="PSAP"
/product="saposin C"
mat_peptide 1219..1467
/gene="PSAP"
/product="saposin D"
variation 656
/gene="PSAP"
/note="c in wt; t in saposin B deficiency (Thr->Ile)"
BASE COUNT 496 a 565 c 610 g 500 t
ORIGIN Chromosome 10, q21-q22.
1 cgcgctatgt acgccctctt cctcctggcc agcctcctgg gcgcggctct agccggcccg
61 gtccttggac tgaaagaatg caccaggggc tcggcagtgt ggtgccagaa tgtgaagacg
121 gcgtccgact gcggggcagt gaagcactgc ctgcagaccg tttggaacaa gccaacagtg
181 aaatcccttc cctgcgacat atgcaaagac gttgtcaccg cagctggtga tatgctgaag
241 gacaatgcca ctgaggagga gatccttgtt tacttggaga agacctgtga ctggcttccg
301 aaaccgaaca tgtctgcttc atgcaaggag atagtggact cctacctccc tgtcatcctg
361 gacatcatta aaggagaaat gagccgtcct ggggaggtgt gctctgctct caacctctgc
421 gagtctctcc agaagcacct agcagagctg aatcaccaga agcagctgga gtccaataag
481 atcccagagc tggacatgac tgaggtggtg gcccccttca tggccaacat ccctctcctc
541 ctctaccctc aggacggccc ccgcagcaag ccccagccaa aggataatgg ggacgtttgc
601 caggactgca ttcagatggt gactgacatc cagactgctg tacggaccaa ctccaccttt
661 gtccaggcct tggtggaaca tgtcaaggag gagtgtgacc gcctgggccc tggcatggcc
721 gacatatgca agaactatat cagccagtat tctgaaattg ctatccagat gatgatgcac
781 atgcaaccca aggagatctg tgcgctggtt gggttctgtg atgaggtgaa agagatgccc
841 atgcagactc tggtccccgc caaagtggcc tccaagaatg tcatccctgc cctggaactg
901 gtggagccca ttaagaagca cgaggtccca gcaaagtctg atgtttactg tgaggtgtgt
961 gaattcctgg tgaaggaggt gaccaagctg attgacaaca acaagactga gaaagaaata
1021 ctcgacgctt ttgacaaaat gtgctcgaag ctgccgaagt ccctgtcgga agagtgccag
1081 gaggtggtgg acacgtacgg cagctccatc ctgtccatcc tgctggagga ggtcagccct
1141 gagctggtgt gcagcatgct gcacctctgc tctggcacgc ggctgcctgc actgaccgtt
1201 cacgtgactc agccaaagga cggtggcttc tgcgaagtgt gcaagaagct ggtgggttat
1261 ttggatcgca acctggagaa aaacagcacc aagcaggaga tcctggctgc tcttgagaaa
1321 ggctgcagct tcctgccaga cccttaccag aagcagtgtg atcagtttgt ggcagagtac
1381 gagcccgtgc tgatcgagat cctggtggag gtgatggatc cttccttcgt gtgcttgaaa
1441 attggagcct gcccctcggc ccataagccc ttgttgggaa ctgagaagtg tatatggggc
1501 ccaagctact ggtgccagaa cacagagaca gcagcccagt gcaatgctgt cgagcattgc
1561 aaacgccatg tgtggaacta ggaggaggaa tattccatct tggcagaaac cacagcattg
1621 gtttttttct acttgtgtgt ctgggggaat gaacgcacag atctgtttga ctttgttata
1681 aaaatagggc tcccccacct cccccatttc tgtgtccttt attgtagcat tgctgtctgc
1741 aagggagccc ctagcccctg gcagacatag ctgcttcagt gccccttttc tctctgctag
1801 atggatgttg atgcactgga ggtcttttag cctgcccttg catggcgcct gctggaggag
1861 gagagagctc tgctggcatg agccacagtt tcttgactgg aggccatcaa ccctcttggt
1921 tgaggccttg ttctgagccc tgacatgtgc ttgggcactg gtgggcctgg gcttctgagg
1981 tggcctcctg ccctgatcag ggaccctccc cgctttcctg ggcctctcag ttgaaccaaa
2041 gcagcaaaac aaaggcagtt ttatatgaaa gattagaagc ctggaataat caggcttttt
2101 aaatgatgta attcccactg taatagcata gggattttgg aagcagctgc tggtggcttg
2161 ggacatcagt g
//