LOCUS HUME2B 2649 bp mRNA linear HUM 28-MAR-1995
DEFINITION Homo sapiens nuclear-encoded mitochondrial branched chain
alpha-keto acid dehydrogenase transacylase subunit (E2b) mRNA,
complete cds.
ACCESSION M27093
VERSION M27093.1
KEYWORDS branched chain alpha-keto acid dehydrogenase; nuclear-encoded
mitochondrial protein; transacylase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2649)
AUTHORS Nobukuni,Y., Mitsubuchi,H., Endo,F. and Matsuda,I.
TITLE Complete primary structure of the transacylase (E2b) subunit of the
human branched chain alpha-keto acid dehydrogenase complex
JOURNAL Biochem. Biophys. Res. Commun. 161 (3), 1035-1041 (1989)
PUBMED 2742576
COMMENT On Mar 29, 1995 this sequence version replaced gi:341635.
Original source text: Homo sapiens (clone library: lambda gt11 of
J.E.Sadler) placenta cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..2649
/db_xref="H-InvDB:HIT000195107"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="placenta"
/clone_lib="lambda gt11 of J.E.Sadler"
gene 1..2649
/gene="E2B"
CDS 14..1447
/gene="E2B"
/EC_number="1.2.4.4"
/codon_start=1
/product="dihydrolipoyl transacylase"
/protein_id="AAA64512.1"
/translation="MLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSH
PHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKAS
VTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQE
IKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKV
EIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDE
IDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLPFPILNASVDENCQNITYK
ASHNIGIAMDTEQGVIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFT
LSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVI
DGATMSRFSNLWKSYLENPAFMLLDLK"
sig_peptide 14..181
/gene="E2B"
mat_peptide 182..1444
/gene="E2B"
/product="dihydrolipoyl transacylase"
/EC_number="1.2.4.4"
/function="catalyzes oxidative decarboxylation of the
alpha-keto acids derived from transamination of the
branched chain amino acids"
BASE COUNT 821 a 478 c 553 g 797 t
ORIGIN
1 ggctgcagtc cgtatgctga gaacctggag caggaatgcg gggaagctga tttgtgttcg
61 ctattttcaa acatgtggta atgttcatgt tttgaagcca aattatgtgt gtttctttgg
121 ttatccttca ttcaagtata gtcatccaca tcacttcctg aaaacaactg ctgctctccg
181 tggacaggtt gttcagttca agctctcaga cattggagaa gggattagag aagtaactgt
241 taaagaatgg tatgtaaaag aaggagatac agtgtctcag tttgatagca tctgtgaagt
301 tcaaagtgat aaagcttctg ttaccatcac tagtcgttat gatggagtca ttaaaaaact
361 ctattataat ctagacgata ttgcctatgt ggggaagcca ttagtagaca tagaaacgga
421 agctttaaaa gattcagaag aagatgttgt tgaaactcct gcagtgtctc atgatgaaca
481 tacacaccaa gagataaagg gccgaaaaac actggcaact cctgcagttc gccgtctggc
541 aatggaaaac aatattaagc tgagtgaagt tgttggctca ggaaaagatg gcagaatact
601 taaagaagat atcctcaact atttggaaaa gcagacagga gctatattgc ctccttcacc
661 caaagttgaa attatgccac ctccaccaaa gccaaaagac atgactgttc ctatactagt
721 atcaaaacct ccggtattca caggcaaaga caaaacagaa cccataaaag gctttcaaaa
781 agcaatggtc aagactatgt ctgcagccct gaagatacct cattttggtt attgtgatga
841 gattgacctt actgaactgg ttaagctccg agaagaatta aaacccattg catttgctcg
901 tggaattaaa ctctccttta tgcctttctt cttaaaggct gcttccttgg gattactacc
961 atttcctatc cttaacgctt ctgtggatga aaactgccag aatataacat ataaggcttc
1021 tcataacatt gggatagcaa tggatactga gcagggtgtg attgtcccta atgtgaaaaa
1081 tgttcagatc tgctctatat ttgacatcgc cactgaactg aaccgcctcc agaaattggg
1141 ctctgtgggt cagctcagca ccactgatct tacaggagga acatttactc tttccaacat
1201 tggatcaatt ggtggtacct ttgccaaacc agtgataatg ccacctgaag tagccattgg
1261 ggcccttgga tcaattaagg ccattccccg atttaaccag aaaggagaag tatataaggc
1321 acagataatg aatgtgagct ggtcagctga tcacagagtt attgatggtg ctacaatgtc
1381 acgcttctcc aatttgtgga aatcctattt agaaaaccca gcttttatgc tactagatct
1441 gaaatgaaga tgataagaca ttcttgaact ttttgagctt ccaaagagta tgtaaaccct
1501 agctgtgcca gcacatgttc atctttacaa tttatagtgt aaatgatttg tatcgtatga
1561 ttaaggatct aagcgcacaa tatttgtcac tgttctatta gactttttat gaaaatgaat
1621 aatggtgtaa tggttctcct ggggctgtca catttatagg tcagagtgtg acttcttaat
1681 atggtgttgt tgtttttgtg gtcaatggct tgaaactggc aagattaaca aaattaggcc
1741 gggcatggtg ctcacgcctg taatcccagc actttgggag gcccaggtgg cggatcacct
1801 gaggttagaa gtttgagacc agcctggcca acatggtgaa acctggcctc tactaaaaat
1861 acaaaaatta gccgggtgtg gtggtgggta cctataatcc cagctacttg ggaggctgag
1921 gcaggagaat cgcttgaacc tgggaggtgg agtcttgcag tgagctgaga tcgtgctatt
1981 gcactccagc ctgggcgaca gagcagacgc catctcaaaa acaaaaaaac aaaattcatg
2041 ttactaaaag acaggtagcc atatacagac agtatatgcc ctattttttt aactgactct
2101 taatgaaacc tttaatttac ttaattaaga aaatggaatt tatatacaaa aatattttcc
2161 atttccgtta ttatgctaat gtgtatgaaa taagtgcaat tatacattct ctttgagata
2221 tccaagagta tattcttgct ctgtatagag aatatcatct gatagtgtct tatttatatt
2281 aattaatgtc tttgaaaagg gaaaagtata aactgggcct taaaattgtc caattatagt
2341 tttataacca gtctattaaa gttggtttgg ttaaaatgga tatagtttta gatttgtggt
2401 atgctttgtt attttcttgg ggaagacctt caccttgcaa acttccctca tgtaaggaag
2461 gtactttaaa tgtagcagcc actgacattt ctttttttaa aaaaaatttg agaagtctac
2521 ttccttttaa cttttttggt cttcagctaa aaaataggaa taagaaatta aggtctattc
2581 cattccttat atcctgggta agaatgtaaa taagaggaga aggaagagtc taatagtaat
2641 tatggatat
//