LOCUS HUMPFKM23 2815 bp mRNA linear HUM 07-JAN-1995
DEFINITION Human muscle phosphofructokinase (PFKM) mRNA, complete cds.
ACCESSION M26066
VERSION M26066.1
KEYWORDS phosphofructokinase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2815)
AUTHORS Sharma,P.M., Reddy,G.R., Vora,S., Babior,B.M. and McLachlan,A.
TITLE Cloning and expression of a human muscle phosphofructokinase cDNA
JOURNAL Gene 77 (1), 177-183 (1989)
PUBMED 2526045
COMMENT Original source text: Human (34 week old) placenta, cDNA to mRNA,
clone HPFKM1.
Draft entry and computer-readable sequence for [1] kindly submitted
by P.M.Sharma, 12-JUL-1989.
FEATURES Location/Qualifiers
source 1..2815
/db_xref="H-InvDB:HIT000195032"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="1cen-q32"
/sex="female"
/tissue_type="placenta"
/dev_stage="34 week old"
gene 1..2815
/gene="PFKM"
mRNA <1..2815
/gene="PFKM"
/product="PFKM mRNA"
CDS 77..2419
/gene="PFKM"
/EC_number="2.7.1.11"
/codon_start=1
/product="phosphofructokinase"
/protein_id="AAA60068.1"
/db_xref="GDB:G00-120-277"
/translation="MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFT
GARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAA
YNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVG
LVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALV
TSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITS
EDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPAC
VVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVR
PPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEE
AGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLEL
MEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRR
VFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGL
VLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAK
AMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQ
WWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV"
BASE COUNT 686 a 657 c 795 g 677 t
ORIGIN 310 bp upstream of PvuII site.
1 cgggcctccc ccctttccca aggacaatct gcaagaaagc agcggcggag gagagctaag
61 actaaaagag tggatcatga cccatgaaga gcaccatgca gccaaaaccc tggggattgg
121 caaagccatt gctgtcttaa cctctggtgg agatgcccaa ggtatgaatg ctgctgtcag
181 ggctgtggtt cgagttggta tcttcaccgg tgcccgtgtc ttctttgtcc atgagggtta
241 tcaaggcctg gtggatggtg gagatcacat caaggaagcc acctgggaga gcgtttcgat
301 gatgcttcag ctgggaggca cggtgattgg aagtgcccgg tgcaaggact ttcgggaacg
361 agaaggacga ctccgagctg cctacaacct ggtgaagcgt gggatcacca atctctgtgt
421 cattgggggt gatggcagcc tcactggggc tgacaccttc cgttctgagt ggagtgactt
481 gttgagtgac ctccagaaag caggtaagat cacagatgag gaggctacga agtccagcta
541 cctgaacatt gtgggcctgg ttgggtcaat tgacaatgac ttctgtggca ccgatatgac
601 cattggcact gactctgccc tgcatcggat catggaaatt gtagatgcca tcactaccac
661 tgcccagagc caccagagga catttgtgtt agaagtaatg ggccgccact gtggatacct
721 ggcccttgtc acctctctgt cctgtggggc cgactgggtt tttattcctg aatgtccacc
781 agatgacgac tgggaggaac acctttgtcg ccgactcagc gagacaagga cccgtggttc
841 tcgtctcaac atcatcattg tggctgaggg tgcaattgac aagaatggaa aaccaatcac
901 ctcagaagac atcaagaatc tggtggttaa gcgtctggga tatgacaccc gggttactgt
961 cttggggcat gtgcagaggg gtgggacgcc atcagccttt gacagaattc tgggcagcag
1021 gatgggtgtg gaagcagtga tggcactttt ggaggggacc ccagataccc cagcctgtgt
1081 agtgagcctc tctggtaacc aggctgtgcg cctgcccctc atggaatgtg tccaggtgac
1141 caaagatgtg accaaggcca tggatgagaa gaaatttgac gaagccctga agctgagagg
1201 ccggagcttc atgaacaact gggaggtgta caagcttcta gctcatgtca gacccccggt
1261 atctaagagt ggttcgcaca cagtggctgt gatgaacgtg ggggctccgg ctgcaggcat
1321 gaatgctgct gttcgctcca ctgtgaggat tggccttatc cagggcaacc gagtgctcgt
1381 tgtccatgat ggtttcgagg gcctggccaa ggggcagata gaggaagctg gctggagcta
1441 tgttgggggc tggactggcc aaggtggctc taaacttggg actaaaagga ctctacccaa
1501 gaagagcttt gaacagatca gtgccaatat aactaagttt aacattcagg gccttgtcat
1561 cattgggggc tttgaggctt acacaggggg cctggaactg atggagggca ggaagcagtt
1621 tgatgagctc tgcatcccat ttgtggtcat tcctgctaca gtctccaaca atgtccctgg
1681 ctcagacttc agcgttgggg ctgacacagc actcaatact atctgcacaa cctgtgaccg
1741 catcaagcag tcagcagctg gcaccaagcg tcgggtgttt atcattgaga ctatgggtgg
1801 ctactgtggc tacctggcta ccatggctgg actggcagct ggggccgatg ctgcctacat
1861 ttttgaggag cccttcacca ttcgagacct gcaggcaaat gttgaacatc tggtgcaaaa
1921 gatgaaaaca actgtgaaaa ggggcttggt gttaaggaat gaaaagtgca atgagaacta
1981 taccactgac ttcattttca acctgtactc tgaggagggg aagggcatct tcgacagcag
2041 gaagaatgtg cttggtcaca tgcagcaggg tgggagccca accccatttg ataggaattt
2101 tgccactaag atgggcgcca aggctatgaa ctggatgtct gggaaaatca aagagagtta
2161 ccgtaatggg cggatctttg ccaatactcc agattcgggc tgtgttctgg ggatgcgtaa
2221 gagggctctg gtcttccaac cagtggctga gctgaaggac cagacagatt ttgagcatcg
2281 aatccccaag gaacagtggt ggctgaaact gaggcccatc ctcaaaatcc tagccaagta
2341 cgagattgac ttggacactt cagaccatgc ccacctggag cacatcaccc ggaagcggtc
2401 cggggaagct gccgtctaaa cctctctgga gtgaggggaa tagattacct gatcatggtc
2461 agctcacacc ctaataagtc cacatcttct cagtgtttta gctgtttttt tcattaggtt
2521 tccttttatt ctgtaccttg cagccatgac cagttctggc caggagctgg aggagcaggc
2581 agtgggtggg agctcctttt aggtagaatt taacatgact tctgccccag ctttatctgt
2641 cacacaaggc tgggcacctc tagtgctact gctagatatc acttactcag ttagaatttt
2701 cctaaaaata agctttattt atttctttgt gataacaaag agtcttggtt cctctactac
2761 ttttactaca gtgacaaatt gtaactacac taataaatgc caactggtca ctgtg
//