LOCUS HUMATP 4479 bp mRNA linear HUM 25-OCT-1994
DEFINITION Human plasma membrane calcium ATPase (hPMCA4) mRNA, complete cds.
ACCESSION M25874
VERSION M25874.1
KEYWORDS ATPase; transmembrane protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4479)
AUTHORS Strehler,E.E., James,P., Fischer,R., Heim,R., Vorherr,T.,
Filoteo,A.G., Penniston,J.T. and Carafoli,E.
TITLE Peptide sequence analysis and molecular cloning reveal two calcium
pump isoforms in the human erythrocyte membrane
JOURNAL J. Biol. Chem. 265 (5), 2835-2842 (1990)
PUBMED 2137451
REFERENCE 2 (bases 1 to 4479)
AUTHORS Strehler,E.E.
TITLE Direct Submission
JOURNAL Submitted (30-JUN-1989) Emanuel E. Strehler, Mayo
Clinic/Foundation, Biochemistry and Molecular Biology, 200 First
Street S.W., Rochester, MN 55905, USA
COMMENT Original source text: Human cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..4479
/db_xref="H-InvDB:HIT000195018"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="lambda [int5.1,t3.1,t5.4c]"
/cell_line="intestinal mucosa teratocarcinoma cell line
hTera2D"
mRNA 1..4479
/product="calcium ATPase"
CDS 398..4015
/codon_start=1
/product="calcium ATPase (hPMCA4) precursor"
/protein_id="AAA50819.1"
/translation="MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQI
NVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQ
DVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVI
IVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGD
LLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGV
NSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDN
EEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRR
PWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHL
DACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGI
SINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVY
TFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVR
TVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAI
AKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQ
EKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADV
GFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVI
VAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISR
TMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQL
FNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWL
WCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRR
GQILWFRGLNRIQTQIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTP
LLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETS
V"
regulatory 4458..4463
/regulatory_class="polyA_signal_sequence"
BASE COUNT 1163 a 1111 c 1138 g 1067 t
ORIGIN
1 gctggttgtt gctaaggttg ctgccatggt aacatgcaca tcctgtttac accttcatct
61 gggcaagttg gtctaagcta ggaacctacc taccctggac aaccactatc atcaccacct
121 ggggacacca atcatcgtga cacggagtcc accttccact cagttccccc atcctcttcc
181 tcctctcgct gccagacttc atagagaaga aaggatctag acttcggacg gctactcggg
241 agcttattgc acaagatata ttcaatctat tccctcactg ggcccccaga gaagcaagaa
301 gtaggaagaa gttgagacag ggaggcagga gacactggtc agttgaaggg aaacgctaca
361 tcttctctgg ttgaggggct tggtaacagc aggcaaaatg acgaacccat cagaccgtgt
421 cttgcctgcc aactcgatgg ccgagagccg tgaaggggac tttggctgca cagtaatgga
481 actgaggaag ctcatggagc tgcgttcgag ggatgcactg acccagatta atgtccacta
541 tggaggtgta cagaatctct gcagtagact gaaaacctcc cctgtggaag gtctgtctgg
601 gaaccctgca gatctggaga aacgtaggca ggtgtttgga cacaacgtga tcccccccaa
661 aaagcccaag actttcttag aattagtgtg ggaagctctt caagatgtca cgctcatcat
721 cctggagatt gcagccatca tctccctggt cctgtccttt tatcgccctg ctggtgaaga
781 aaatgaactg tgtggtcaag tcgcaactac cccagaagat gaaaatgagg cacaagctgg
841 ctggattgag ggggcagcca tccttttctc agtgatcatc gtggtgttag tgactgcctt
901 taatgattgg agcaaagaga agcaattccg ggggctgcag tgccgcattg aacaggagca
961 aaagttctcc atcatccgaa acggtcaact catccagctc cctgtggctg agattgtggt
1021 tggtgatatt gcccaagtca aatacggtga tctgctgcct gcagatggaa tcctgatcca
1081 agggaatgat ctgaagattg atgagagctc tctgacaggg gaatctgacc atgtcaagaa
1141 gtccctggac aaagacccca tgttgctctc agggacccat gtcatggaag gttctggccg
1201 gatggtggtg acagctgttg gtgtcaactc tcagactgga atcatcctta ctctcttggg
1261 ggtcaatgag gatgacgaag gggagaaaaa gaagaaaggt aaaaaacaag gagtccctga
1321 aaatcgcaac aaagcaaaga cccaagacgg agtggccctg gaaatccagc cactcaacag
1381 ccaggaggga atcgacaatg aggaaaagga caagaaggca gtcaaggtgc ctaaaaagga
1441 gaagtcagtg ctgcagggca agctgactcg cctggctgtt cagattggga aagccggtct
1501 gctcatgtct gctctcacgg ttttcatcct gattctatac tttgtgattg acaactttgt
1561 gataaatcgc agaccatggc tccctgagtg tactcccatc tacatccagt actttgtcaa
1621 gttcttcatc atcggcatca ctgtactggt ggtggctgtg ccagaggggc tgcctctggc
1681 tgtcaccatc tcactggcct actctgtgaa gaaaatgatg aaagacaata acctagtacg
1741 gcacttggat gcttgtgaga ccatgggcaa cgccaccgcc atctgctctg ataagacagg
1801 cacgttgacc atgaaccgca tgactgtggt acaagcttat attgggggca tccattaccg
1861 tcaaatccca agccctgatg tcttcctgcc caaagtcctg gacctcattg tcaatggcat
1921 ttctatcaac agtgcttata cctccaagat tctgcctcca gagaaggagg gaggcctgcc
1981 tcggcaggtg ggcaacaaga ccgagtgtgc tctgctaggc tttgtcacag atctgaagca
2041 ggattatcag gctgtgcgta atgaagtgcc cgaggagaag ctctacaagg tgtacacctt
2101 taactcagtg cgcaagtcaa tgagcaccgt catcaggaat cccaacggtg gcttccgtat
2161 gtacagcaag ggcgcctctg agatcatctt gcgcaagtgt aatcgaatcc tggaccggaa
2221 aggggaagca gtgccattca agaataaaga cagagatgat atggtacgca ctgtcatcga
2281 gcccatggcc tgtgatggac tccggactat ctgcatagct taccgggact tcgatgacac
2341 agagccctct tgggacaatg agaatgagat cctcaccgaa ctgacctgta tcgcggtggt
2401 gggcattgag gaccctgtgc gcccagaggt gccagatgct attgccaaat gcaaacaagc
2461 tggcattact gtcagaatgg tgacaggtga caacatcaac acagcccggg ccattgccac
2521 caaatgtggc attctgacac ctggggatga cttcctgtgc ttagaaggca aagaattcaa
2581 ccggctcatc cgcaacgaga aaggcgaggt agagcaagaa aagctggaca agatctggcc
2641 taagcttcgg gtcctggcgc gatcttctcc cactgacaag cacaccctgg tgaaaggcat
2701 aattgacagc actgttgggg aacaccggca ggtcgtggct gtcactggtg atggcacaaa
2761 tgacgggcct gctctgaaga aagcggatgt tggttttgcc atgggcatcg caggcacaga
2821 tgtagcaaag gaggcttcag acatcatcct aacagatgac aacttcacca gcattgtgaa
2881 ggcagtgatg tggggacgaa atgtctatga cagcatctcc aagttcctgc agttccagct
2941 cactgtcaat gtggtggccg tgattgtagc cttcactgga gcctgtatca ctcaggattc
3001 cccattgaaa gctgtgcaga tgttgtgggt taatctgatc atggacactt ttgcttcatt
3061 ggccctggcc acagagcccc ctacggaatc tctgttgaag cggcgcccct atggccgaaa
3121 taagcctctg atctcacgca ctatgatgaa gaacatcttg ggccatgcat tctatcagct
3181 cattgtcatc tttatccttg tctttgcggg tgagaaattc tttgatattg atagtgggag
3241 gaaggcacct ctacattcac cacccagcca gcactatacc attgttttta acaccttcgt
3301 gctgatgcag ctcttcaatg aaatcaactc ccgaaagatc catggagaga agaacgtctt
3361 ttcaggcatc taccgcaaca ttatcttctg ctctgtagtc ttgggcacat tcatctgcca
3421 gattttcatc gtggaatttg ggggtaaacc cttcagttgt acaagcctca gcctgtctca
3481 gtggctgtgg tgtctcttca ttgggattgg agaacttctg tggggccagt tcatctccgc
3541 aatacctacc cgatccctga agttcctgaa ggaggctggg catggcacca ccaaagagga
3601 gatcaccaag gatgccgagg gactggatga gattgaccat gctgagatgg agctgcgccg
3661 aggccagatc ctctggttcc ggggcctgaa ccgtatccag actcagatca aagtggtcaa
3721 agcgttccat agttccctcc acgaaagcat tcagaaaccc tacaaccaaa agtccatcca
3781 cagcttcatg acccaccctg aattcgccat agaggaggag ttgccacgaa caccactcct
3841 ggatgaggaa gaggaggaaa atcctgacaa ggcttctaag tttgggacta gggtgctcct
3901 gttggatggt gaggtcactc catatgccaa tacaaacaac aatgcggtgg attgcaacca
3961 agtgcagctc ccccagtcgg acagctctct acagagccta gagacatcag tttgaatttt
4021 ctttctctga ctctcatccc tattttacct atttccattt tcgtctgtcc catctatgag
4081 gtgatgatgg gacttttcat cgtcacgtca gctgctgtgt taacagcagt gtgtgtgaag
4141 tgaacctcta cctgaccatg aagaggcaag agcacagact tacaaggatt tcaacttaag
4201 cttgacttgg ggtttgtagc gggacccagt caaaccccat tcaggtcatg gtagaatcta
4261 ctccttggta gtcacttgtc atttttaaag aaatgatgac aatcctcttg gcatcacccc
4321 accccacatt ctccccgatg ttcctctcct gaattctgga ttttgtccta caagtctgtg
4381 ccatttataa gctaagttga gggttcagag ggagacaaat atccccaagt gaaatatgtt
4441 gcggtagaga tggaatgtat aaaatccgcc agtagtgtg
//