LOCUS HUMNCF1A 1339 bp mRNA linear HUM 14-DEC-1994
DEFINITION Human neutrophil cytosol factor 1 (NCF-47k) protein, complete cds.
ACCESSION M25665 M26193
VERSION M25665.1
KEYWORDS neutrophil cytosol factor 1.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1339)
AUTHORS Volpp,B.D., Nauseef,W.M., Donelson,J.E., Moser,D.R. and Clark,R.A.
TITLE Cloning of the cDNA and functional expression of the 47-kilodalton
cytosolic component of human neutrophil respiratory burst oxidase
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86 (18), 7195-7199 (1989)
PUBMED 2550933
REMARK Erratum:[Proc Natl Acad Sci U S A 1989 Dec;86(23):9563]
REFERENCE 2 (bases 1 to 1339)
AUTHORS Volpp,B.D., Nauseef,W.M. and Clark,R.A.
TITLE Correction
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 9563-9563 (1989)
REFERENCE 3 (bases 1 to 1339)
AUTHORS Lomax,K.J., Leto,T.L., Nunoi,H., Gallin,J.I. and Malech,H.L.
TITLE Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in
chronic granulomatous disease
JOURNAL Science 245 (4916), 409-412 (1989)
PUBMED 2547247
REMARK Erratum:[Science 1989 Nov 24;246(4933):987]
COMMENT Original source text: Human promyelocytic leukemia HL60 cell line,
cDNA to mRNA, clone 8A.
[2] revises [3],[1].
Draft entry and computer-readable sequence for [3] kindly submitted
by H.L.Malech, 21-JUN-1989, and [1] by B.D.Volpp, 14-JUL-1989.
FEATURES Location/Qualifiers
source 1..1339
/db_xref="H-InvDB:HIT000195006"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="7q11.23"
/cell_line="HL60"
/tissue_type="promyelocytic leukemia"
gene 1..1339
/gene="NCF1"
mRNA <1..1339
/gene="NCF1"
/note="G00-120-222"
CDS 13..1185
/gene="NCF1"
/codon_start=1
/product="neutrophil cytosol factor 1"
/protein_id="AAA57209.1"
/db_xref="GDB:G00-120-222"
/translation="MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRF
TEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLM
SLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQT
YRAIADYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDE
TEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFP
SMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQ
RRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV"
regulatory 1319..1324
/regulatory_class="polyA_signal_sequence"
/gene="NCF1"
polyA_site 1339
/gene="NCF1"
BASE COUNT 287 a 441 c 394 g 217 t
ORIGIN
1 ggccacccag tcatggggga caccttcatc cgtcacatcg ccctgctggg ctttgagaag
61 cgcttcgtac ccagccagca ctatgtgtac atgttcctgg tgaaatggca ggacctgtcg
121 gagaaggtgg tctaccggcg cttcaccgag atctacgagt tccataaaac cttaaaagaa
181 atgttcccta ttgaggcagg ggcgatcaat ccagagaaca ggatcatccc ccacctccca
241 gctcccaagt ggtttgacgg gcagcgggcc gccgagaacc gccagggcac acttaccgag
301 tactgcagca cgctcatgag cctgcccacc aagatctccc gctgtcccca cctcctcgac
361 ttcttcaagg tgcgccctga tgacctcaag ctccccacag acaaccagac aaaaaagcca
421 gagacatact tgatgcccaa agatggcaag agtaccgcga cagacatcac cggccccatc
481 atcctgcaga cgtaccgcgc cattgccgac tacgagaaga cctcgggctc cgagatggct
541 ctgtccacgg gggacgtggt ggaggtcgtg gagaagagcg agagcggttg gtggttctgt
601 cagatgaaag caaagcgagg ctggatccca gcatccttcc tcgagcccct ggacagtcct
661 gacgagacgg aagaccctga gcccaactat gcaggtgagc catacgtcgc catcaaggcc
721 tacactgctg tggaggggga cgaggtgtcc ctgctcgagg gtgaagctgt tgaggtcatt
781 cacaagctcc tggacggctg gtgggtcatc aggaaagacg acgtcacagg ctactttccg
841 tccatgtacc tgcaaaagtc ggggcaagac gtgtcccagg cccaacgcca gatcaagcgg
901 ggggcgccgc cccgcaggtc gtccatccgc aacgcgcaca gcatccatca gcggtcgcgg
961 aagcgcctca gccaggacgc ctatcgccgc aacagcgtcc gttttctgca gcagcgacgc
1021 cgccaggcgc ggccgggacc gcagagcccc gggagcccgc tcgaggagga gcggcagacg
1081 cagcgctcta aaccgcagcc ggcggtgccc ccgcggccga gcgccgacct catcctgaac
1141 cgctgcagcg agagcaccaa gcggaagctg gcgtctgccg tctgaggctg gagcgcagtc
1201 cccagctagc gtctcggccc ttgccgcccc gtgcctgtac atacgtgttc tatagagcct
1261 ggcgtctgga cgccgagggc agccccgacc cctgtccagc gcggctcccg ccaccctcaa
1321 taaatgttgc ttggagtgg
//