LOCUS HUMADH2BA 1618 bp mRNA linear HUM 01-NOV-1994
DEFINITION Human class I alcohol dehydrogenase (ADH2) beta-1 subunit mRNA,
complete cds.
ACCESSION M24317 K01883 M11831 M11832 M11833 M11834 M11835 M11836 M11837
M11838 M11839
VERSION M24317.1
KEYWORDS alcohol dehydrogenase; dehydrogenase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1618)
AUTHORS Ikuta,T., Fujiyoshi,T., Kurachi,K. and Yoshida,A.
TITLE Molecular cloning of a full-length cDNA for human alcohol
dehydrogenase
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (9), 2703-2707 (1985)
PUBMED 2986130
REFERENCE 2 (bases 733 to 738)
AUTHORS Ikuta,T., Fujiyoshi,T., Kurachi,K. and Yoshida,A.
TITLE Correction: Molecular cloning of a full-length cDNA for human
alcohol dehydrogenase
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 5578-5578 (1985)
REFERENCE 3 (bases 164 to 183; 1132 to 1148)
AUTHORS Xu,Y.L., Carr,L.G., Bosron,W.F., Li,T.K. and Edenberg,H.J.
TITLE Genotyping of human alcohol dehydrogenases at the ADH2 and ADH3
loci following DNA sequence amplification
JOURNAL Genomics 2 (3), 209-214 (1988)
PUBMED 3397059
COMMENT Original source text: Human liver, cDNA to mRNA, (library of Woo)
clone lambda-ADH14.
[2] revises [1].
Reprint of [1] kindly provided by A.Yoshida, 15-SEP-1985. Draft
entry and clean copy sequence for [2] kindly provided by G.Duester,
31-MAR-1986.
ADH is encoded by at least five genes that fall into three classes.
Class I, which evolved as a multigene family, contains a large
group of isoenzymes possessing various combinations of alpha, beta,
and gamma subunits, coded for by three genes designated ADH1, ADH2,
and ADH3, respectively. Class II contains the pi isoenzyme and
class III contains the chi isoenzyme. The majority of Orientals
have the 'atypical' 2-ADH-2 gene, producing an atypical beta-2
subunit instead of the beta-1 subunit, which is predominant in
Caucasians.
The other human class I ADH1 beta subunit sequences are found under
accession D00137 X03350 M24308-M24316 M21692.
FEATURES Location/Qualifiers
source 1..1618
/db_xref="H-InvDB:HIT000194923"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="4q21-q23"
gene 1..1618
/gene="ADH2"
CDS 31..1158
/gene="ADH2"
/note="alcohol dehydrogenase beta subunit"
/codon_start=1
/protein_id="AAA51884.1"
/db_xref="GDB:G00-119-651"
/translation="MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAV
GICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCR
VCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAK
IDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAA
RIIAVDINKDKKAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTM
MASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVA
DFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF"
variation 173
/gene="ADH2"
/note="g in ADH2-1; a in ADH2-2"
old_sequence 733..738
/gene="ADH2"
/citation=[1]
variation 1137
/gene="ADH2"
/note="c in ADH2-1; t in ADH2-3"
BASE COUNT 437 a 353 c 401 g 427 t
ORIGIN 67 bp upstream of PvuII site; chromosome 4q21-q25.
1 ctgctggtgg gcagagaaga cagaaacgac atgagcacag caggaaaagt aatcaaatgc
61 aaagcagctg tgctatggga ggtaaagaaa cccttttcca ttgaggatgt ggaggttgca
121 cctcctaagg cttatgaagt tcgcattaag atggtggctg taggaatctg tcgcacagat
181 gaccacgtgg ttagtggcaa cctggtgacc ccccttcctg tgattttagg ccatgaggca
241 gccggcatcg tggagagtgt tggagaaggg gtgactacag tcaaaccagg tgataaagtc
301 atcccgctct ttactcctca gtgtggaaaa tgcagagttt gtaaaaaccc ggagagcaac
361 tactgcttga aaaatgatct aggcaatcct cgggggaccc tgcaggatgg caccaggagg
421 ttcacctgca gggggaagcc cattcaccac ttccttggca ccagcacctt ctcccagtac
481 acggtggtgg atgagaatgc agtggccaaa attgatgcag cctcgcccct ggagaaagtc
541 tgcctcattg gctgtggatt ctcgactggt tatgggtctg cagttaacgt tgccaaggtc
601 accccaggct ctacctgtgc tgtgtttggc ctgggagggg tcggcctatc tgctgttatg
661 ggctgtaaag cagctggagc agccagaatc attgcggtgg acatcaacaa ggacaaaaaa
721 gcaaaggcca aagagttggg tgccactgaa tgcatcaacc ctcaagacta caagaaaccc
781 atccaggaag tgctaaagga aatgactgat ggaggtgtgg atttttcgtt tgaagtcatc
841 ggtcggcttg acaccatgat ggcttccctg ttatgttgtc atgaggcatg tggcacaagc
901 gtcatcgtag gggtacctcc tgcttcccag aacctctcaa taaaccctat gctgctactg
961 actggacgca cctggaaggg ggctgtttat ggtggcttta agagtaaaga aggtatccca
1021 aaacttgtgg ctgattttat ggctaagaag ttttcactgg atgcgttaat aacccatgtt
1081 ttaccttttg aaaaaataaa tgaaggattt gacctgcttc actctgggaa aagtatccgt
1141 accgtcctga cgttttgagg caatagagat gccttcccct gtagcagtct tcagcctcct
1201 ctaccctaca agatctggag caacagctag gaaatatcat taattcagct cttcagagat
1261 gttatcaata aattacacat gggggctttc caaagaaatg gaaattgatg ggaaattatt
1321 tttcaggaaa atttaaaatt caagtgagaa gtaaataaag tgttgaacat cagctgggga
1381 attgaagcca acaaaccttc cttcttaacc attctactgt gtcacctttg ccattgagga
1441 aaaatattcc tgtgacttct tgcatttttg gtatcttcat aatctttagt catcgaatcc
1501 cagtggaggg gaccctttta cttgccctga acatacacat gctgggccat tgtgattgaa
1561 gtcttctaac tctgtctcag ttttcactgt cgacattttc ctttttctaa taaaaatg
//