LOCUS HUMIDE 3337 bp mRNA linear HUM 08-NOV-1994
DEFINITION Human insulin-degrading enzyme (IDE) mRNA, complete cds.
ACCESSION M21188
VERSION M21188.1
KEYWORDS insulin-degrading enzyme; proteinase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3337)
AUTHORS Affholter,J.A., Fried,V.A. and Roth,R.A.
TITLE Human insulin-degrading enzyme shares structural and functional
homologies with E. coli protease III
JOURNAL Science 242 (4884), 1415-1418 (1988)
PUBMED 3059494
REFERENCE 2 (sites)
AUTHORS Affholter,J.A., Hsieh,C.L., Francke,U. and Roth,R.A.
TITLE Insulin-degrading enzyme: stable expression of the human
complementary DNA, characterization of its protein product, and
chromosomal mapping of the human and mouse genes
JOURNAL Mol. Endocrinol. 4 (8), 1125-1135 (1990)
PUBMED 2293021
COMMENT Original source text: Homo sapiens hepatoma cDNA to mRNA.
[2] revises [1].
Computer readable copy of sequence [1] kindly provided by R.A.Roth,
02-NOV-1988.
FEATURES Location/Qualifiers
source 1..3337
/db_xref="H-InvDB:HIT000194776"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="10"
/cell_type="lymphocyte"
/tissue_type="hepatoma"
gene 1..3337
/gene="IDE"
mRNA <1..3279
/gene="IDE"
/note="G00-118-817"
CDS 58..3117
/gene="IDE"
/codon_start=1
/product="insulin-degrading enzyme"
/protein_id="AAA52712.1"
/db_xref="GDB:G00-118-817"
/translation="MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNN
PAIKRIGNHITKSPEDKREYRGLELANGIKVLLMSDPTTDKSSAALDVHIGSLSDPPN
IAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG
ALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF
GTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFS
EVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYL
GHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIL
HMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVL
TAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDE
VIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTVMSKLWFKQDDK
KKKPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTI
YGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHA
MYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALG
IMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTD
MQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQSLRFIIQSEKPP
HYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQ
QYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEF
PCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL"
BASE COUNT 1060 a 694 c 694 g 889 t
ORIGIN Chromosome 10./kastern.
1 ccggctcgaa gcgcaacgag gaagcgtttg cggtgatccc ggcgactgcg ctggctaatg
61 cggtaccggc tagcgtggct tctgcacccc gcactgccca gcaccttccg ctcagtcctc
121 ggcgcccgcc tgccgcctcc ggagcgcctg tgtggtttcc aaaaaaagac ttacagcaaa
181 atgaataatc cagccatcaa gagaatagga aatcacatta ccaagtctcc tgaagacaag
241 cgagaatatc gagggctaga gctggccaat ggtatcaaag tacttcttat gagtgatccc
301 accacggata agtcatcagc agcacttgat gtgcacatag gttcattgtc ggatcctcca
361 aatattgctg gcttaagtca tttttgtgaa catatgcttt ttttgggaac aaagaaatac
421 cctaaagaaa atgaatacag ccagtttctc agtgagcatg caggaagttc aaatgccttt
481 actagtggag agcataccaa ttactatttt gatgtttctc atgaacacct agaaggtgcc
541 ctagacaggt ttgcacagtt ttttctgtgc cccttgttcg atgaaagttg caaagacaga
601 gaggtgaatg cagttgattc agaacatgag aagaatgtga tgaatgatgc ctggagactc
661 tttcaattgg aaaaagctac agggaatcct aaacacccct tcagtaaatt tgggacaggt
721 aacaaatata ctctggagac tagaccaaac caagaaggca ttgatgtaag acaagagcta
781 ctgaaattcc attctgctta ctattcatcc aacttaatgg ctgtttgtgt tttaggtcga
841 gaatctttag atgacttgac taatctggtg gtaaagttat tttctgaagt agagaacaaa
901 aatgttccat tgccagaatt tcctgaacac cctttccaag aagaacatct taaacaactt
961 tacaaaatag tacccattaa agatattagg aatctctatg tgacatttcc catacctgac
1021 cttcagaaat actacaaatc aaatcctggt cattatcttg gtcatctcat tgggcatgaa
1081 ggtcctggaa gtctgttatc agaacttaag tcaaagggct gggttaatac tcttgttggt
1141 gggcagaagg aaggagcccg aggttttatg ttttttatca ttaatgtgga cttgaccgag
1201 gaaggattat tacatgttga agatataatt ttgcacatgt ttcaatacat tcagaagtta
1261 cgtgcagaag gacctcaaga atgggttttc caagagtgca aggacttgaa tgctgttgct
1321 tttaggttta aagacaaaga gaggccacgg ggctatacat ctaagattgc aggaatattg
1381 cattattatc ccctagaaga ggtgctcaca gcggaatatt tactggaaga atttagacct
1441 gacttaatag agatggttct cgataaactc agaccagaaa atgtccgggt tgccatagtt
1501 tctaaatctt ttgaaggaaa aactgatcgc acagaagagt ggtatggaac ccagtacaaa
1561 caagaagcta taccggatga agtcatcaag aaatggcaaa atgctgacct gaatgggaaa
1621 tttaaacttc ctacaaagaa tgaatttatt cctacgaatt ttgagatttt accgttagaa
1681 aaagaggcga caccataccc tgctcttatt aaggatacag tcatgagcaa actttggttc
1741 aaacaagatg ataagaaaaa aaagccgaag gcttgtctca actttgaatt tttcagccca
1801 tttgcttatg tggacccctt gcactgtaac atggcctatt tgtaccttga gctcctcaaa
1861 gactcactca acgagtatgc atatgcagca gagctagcag gcttgagcta tgatctccaa
1921 aataccatct atgggatgta tctttcagtg aaaggttaca atgacaagca gccaatttta
1981 ctaaagaaga ttattgagaa aatggctacc tttgagattg atgaaaaaag atttgaaatt
2041 atcaaagaag catatatgcg atctcttaac aatttccggg ctgaacagcc tcaccagcat
2101 gccatgtact acctccgctt gctgatgact gaagtggcct ggactaaaga tgagttaaaa
2161 gaagctctgg atgatgtaac ccttcctcgc cttaaggcct tcatacctca gctcctgtca
2221 cggctgcaca ttgaagccct tctccatgga aacataacaa agcaggctgc attaggaatt
2281 atgcagatgg ttgaagacac cctcattgaa catgctcata ccaaacctct ccttccaagt
2341 cagctggttc ggtatagaga agttcagctc cctgacagag gatggtttgt ttatcagcag
2401 agaaatgaag ttcacaataa ctgtggcatc gagatatact accaaacaga catgcaaagc
2461 acctcagaga atatgtttct ggagctcttc tgtcagatta tctcggaacc ttgcttcaac
2521 accctgcgca ccaaggagca gttgggctat atcgtcttca gcgggccacg tcgagctaat
2581 ggcatacaga gcttgagatt catcatccag tcagaaaagc cacctcacta cctagaaagc
2641 agagtggaag ctttcttaat taccatggaa aagtccatag aggacatgac agaagaggcc
2701 ttccaaaaac acattcaggc attagcaatt cgtcgactag acaaaccaaa gaagctatct
2761 gctgagtgtg ctaaatactg gggagaaatc atctcccagc aatataattt tgacagagat
2821 aacactgagg ttgcatattt aaagacactt accaaggaag atatcatcaa attctacaag
2881 gaaatgttgg cagtagatgc tccaaggaga cataaggtat ccgtccatgt tcttgccagg
2941 gaaatggatt cttgtcctgt tgttggagag ttcccatgtc aaaatgacat aaatttgtca
3001 caagcaccag ccttgccaca acctgaagtg attcagaaca tgaccgaatt caagcgtggt
3061 ctgccactgt ttccccttgt gaaaccacat attaacttca tggctgcaaa actctgaaga
3121 ttccccatgc atgggaaagt gcaagtggat gcattcctga gtcttccaga gcctaagaaa
3181 atcatcttgg ccactttaat agtttctgat tcactattag agaaacaaac aaaaaattgt
3241 caaatgtcat tatgtagaaa tattataaat ccaaagtaaa ttacaaaatc ttatagatgt
3301 agaatatttt ttaaatacat gccctcttaa atatttc
//