LOCUS HUMKAD 1521 bp mRNA linear HUM 06-JAN-1995
DEFINITION Human branched-chain alpha-keto acid dehydrogenase (E2) mRNA,
complete cds.
ACCESSION M19301
VERSION M19301.1
KEYWORDS alpha-keto acid dehydrogenase; transacylase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1521)
AUTHORS Lau,K.S., Griffin,T.A., Hu,C.W. and Chuang,D.T.
TITLE Conservation of primary structure in the lipoyl-bearing and
dihydrolipoyl dehydrogenase binding domains of mammalian
branched-chain alpha-keto acid dehydrogenase complex: molecular
cloning of human and bovine transacylase (E2) cDNAs
JOURNAL Biochemistry 27 (6), 1972-1981 (1988)
PUBMED 2837277
COMMENT Original source text: Human (foetus) liver, cDNA to mRNA, clone
hE2-1.
Draft entry and printed copy of sequence for [1] kindly provided by
K.S.Lau, 18-MAY-1988.
FEATURES Location/Qualifiers
source 1..1521
/db_xref="H-InvDB:HIT000194683"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="1p31"
gene 1..1521
/gene="DBT"
mRNA <1..1521
/gene="DBT"
/product="KAD mRNA"
CDS 14..961
/gene="DBT"
/note="alpha-keto acid dehydrogenase precursor"
/codon_start=1
/protein_id="AAA59200.1"
/db_xref="GDB:G00-118-784"
/translation="MLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSH
PHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKAS
VTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQE
IKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKV
EIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDE
IDLTELVKLREELKPIAFARGIKLSFMPFFLKYYFSWKS"
sig_peptide 14..181
/gene="DBT"
/note="alpha-keto acid dehydrogenase signal peptide"
mat_peptide 182..958
/gene="DBT"
/product="alpha-keto acid dehydrogenase"
BASE COUNT 478 a 277 c 295 g 471 t
ORIGIN 7 bp upstream of PstI site.
1 cgctgcagtc cgtatgctga gaacctggag caggaatgcg gggaagctga tttgtgttcg
61 ctattttcaa acatgtggta atgttcatgt tttgaagcca aattatgtgt gtttctttgg
121 ttatccttca ttcaagtata gtcatccaca tcacttcctg aaaacaactg ctgctctccg
181 tggacaggtt gttcagttca agctctcaga cattggagaa gggattagag aagtaactgt
241 taaagaatgg tatgtaaaag aaggagatac agtgtctcag tttgatagca tctgtgaagt
301 tcaaagtgat aaagcttctg ttaccatcac tagtcgttat gatggagtca ttaaaaaact
361 ctattataat ctagacgata ttgcctatgt ggggaagcca ttagtagaca tagaaacgga
421 agctttaaaa gattcagaag aagatgttgt tgaaactcct gcagtgtctc atgatgaaca
481 tacacaccaa gagataaagg gccgaaaaac actggcaact cctgcagttc gccgtctggc
541 aatggaaaac aatattaagc tgagtgaagt tgttggctca ggaaaagatg gcagaatact
601 taaagaagat atcctcaact atttggaaaa gcagacagga gctatattgc ctccttcacc
661 caaagttgaa attatgccac ctccaccaaa gccaaaagac atgactgttc ctatactagt
721 atcaaaacct ccggtattca caggcaaaga caaaacagaa cccataaaag gctttcaaaa
781 agcaatggtc aagactatgt ctgcagccct gaagatacct cattttggtt attgtgatga
841 gattgacctt actgaactgg ttaagctccg agaagaatta aaacccattg catttgctcg
901 tggaattaaa ctctccttta tgcctttctt cttaaagtat tacttctcat ggaaatctta
961 agatcaacat gtcagtgagg aaatcaccta gaatttagac attttctttg gaactttggc
1021 tggtctgtat cattattttg aaaatctcct tgatgtttac aagctacctg actactttat
1081 cttttattct ttaatgatta gggtgaccta gttccacatg gcctgaaggt aacattggca
1141 gtaatgtgac taggtacatg tagacttgaa attaaactca gcataaataa gagttctctc
1201 tctccctcct tttatacctc ttattgtttc aaattctcta tcctttctaa gttaaaagta
1261 ctaaaattgc attctttttc tgttaaacag gctgcttcct tgggattact acagtttcct
1321 atccttaacg cttctgtgga tgaaaactgc cacaatataa catataaggt tggctatgga
1381 aggagtaaaa atgttcttat tattaataga agatggggca aatgtgcttg tagattagag
1441 acttctattg tacctcaaat agtgatcttt tttagactag aattaaaggt atggggtaaa
1501 acattaaact taagtctaat t
//