LOCUS HUMRBS 4740 bp mRNA linear HUM 12-JUL-1995
DEFINITION Human retinoblastoma susceptibility mRNA, complete cds.
ACCESSION M15400
VERSION M15400.1
KEYWORDS retinoblastoma susceptibility.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 243 to 4740)
AUTHORS Lee,W.H., Bookstein,R., Hong,F., Young,L.J., Shew,J.Y. and Lee,E.Y.
TITLE Human retinoblastoma susceptibility gene: cloning, identification,
and sequence
JOURNAL Science 235 (4794), 1394-1399 (1987)
PUBMED 3823889
REFERENCE 2 (bases 1 to 480)
AUTHORS Lee,E.Y., Bookstein,R., Young,L.J., Lin,C.J., Rosenfeld,M.G. and
Lee,W.H.
TITLE Molecular mechanism of retinoblastoma gene inactivation in
retinoblastoma cell line Y79
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 85 (16), 6017-6021 (1988)
PUBMED 3413073
COMMENT Original source text: Homo sapiens (clone: RB-[1,5]) foetus retina
cDNA to mRNA.
Draft entry and computer-readable copy of sequence in [1] kindly
provided by R.Bookstein, 27-APR-1987.
FEATURES Location/Qualifiers
source 1..4740
/db_xref="H-InvDB:HIT000194394"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="13q14.2"
/clone="RB-[1,5]"
/tissue_type="retina"
/dev_stage="foetus"
gene 1..4740
/gene="RB1"
mRNA <1..4740
/gene="RB1"
/product="rbs mRNA"
CDS 139..2925
/gene="RB1"
/note="retinoblastoma susceptibility protein"
/codon_start=1
/protein_id="AAA69807.1"
/db_xref="GDB:G00-118-734"
/translation="MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPL
VRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGIC
IFIARVDLDEMSFTLLSYRKTYEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFA
LFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQL
MLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEV
LCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDRR
LFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNIIPPHTPVRTVMNTIQQLMMILNSA
SDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGV
RLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDS
GTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSD
SPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNS
TANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWT
LFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFK
RVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLR
IPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSD
RVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQ
KMNDSMDTSNKEEK"
BASE COUNT 1508 a 887 c 862 g 1483 t
ORIGIN 1038 bp upstream of EcoRI site; chromosome 13q14.1-q14.2.
1 ttccggtttt tctcagggga cgttgaaatt atttttgtaa cgggagtcgg gagaggacgg
61 ggcgtgcccc gcgtgcgcgc gcgtcgtcct ccccggcgct cctccacagc tcgctggctc
121 ccgccgcgga aaggcgtcat gccgcccaaa accccccgaa aaacggccgc caccgccgcc
181 gctgccgccg cggaaccccc ggcaccgccg ccgccgcccc ctcctgagga ggacccagag
241 caggacagcg gcccggagga cctgcctctc gtcaggcttg agtttgaaga aacagaagaa
301 cctgatttta ctgcattatg tcagaaatta aagataccag atcatgtcag agagagagct
361 tggttaactt gggagaaagt ttcatctgtg gatggagtat tgggaggtta tattcaaaag
421 aaaaaggaac tgtggggaat ctgtatcttt attgcacgag ttgacctaga tgagatgtcg
481 ttcactttac tgagctacag aaaaacatac gaaatcagtg tccataaatt ctttaactta
541 ctaaaagaaa ttgataccag taccaaagtt gataatgcta tgtcaagact gttgaagaag
601 tatgatgtat tgtttgcact cttcagcaaa ttggaaagga catgtgaact tatatatttg
661 acacaaccca gcagttcgat atctactgaa ataaattctg cattggtgct aaaagtttct
721 tggatcacat ttttattagc taaaggggaa gtattacaaa tggaagatga tctggtgatt
781 tcatttcagt taatgctatg tgtccttgac tattttatta aactctcacc tcccatgttg
841 ctcaaagaac catataaaac agctgttata cccattaatg gttcacctcg aacacccagg
901 cgaggtcaga acaggagtgc acggatagca aaacaactag aaaatgatac aagaattatt
961 gaagttctct gtaaagaaca tgaatgtaat atagatgagg tgaaaaatgt ttatttcaaa
1021 aattttatac cttttatgaa ttctcttgga cttgtaacat ctaatggact tccagaggtt
1081 gaaaatcttt ctaaacgata cgaagaaatt tatcttaaaa ataaagatct agatcgaaga
1141 ttatttttgg atcatgataa aactcttcag actgattcta tagacagttt tgaaacacag
1201 agaacaccac gaaaaagtaa ccttgatgaa gaggtgaata taattcctcc acacactcca
1261 gttaggactg ttatgaacac tatccaacaa ttaatgatga ttttaaattc tgcaagtgat
1321 caaccttcag aaaatctgat ttcctatttt aacaactgca cagtgaatcc aaaagaaagt
1381 atactgaaaa gagtgaagga tataggatac atctttaaag agaaatttgc taaagctgtg
1441 ggacagggtt gtgtcgaaat tggatcacag cgatacaaac ttggagttcg cttgtattac
1501 cgagtaatgg aatccatgct taaatcagaa gaagaacgat tatccattca aaattttagc
1561 aaacttctga atgacaacat ttttcatatg tctttattgg cgtgcgctct tgaggttgta
1621 atggccacat atagcagaag tacatctcag aatcttgatt ctggaacaga tttgtctttc
1681 ccatggattc tgaatgtgct taatttaaaa gcctttgatt tttacaaagt gatcgaaagt
1741 tttatcaaag cagaaggcaa cttgacaaga gaaatgataa aacatttaga acgatgtgaa
1801 catcgaatca tggaatccct tgcatggctc tcagattcac ctttatttga tcttattaaa
1861 caatcaaagg accgagaagg accaactgat caccttgaat ctgcttgtcc tcttaatctt
1921 cctctccaga ataatcacac tgcagcagat atgtatcttt ctcctgtaag atctccaaag
1981 aaaaaaggtt caactacgcg tgtaaattct actgcaaatg cagagacaca agcaacctca
2041 gccttccaga cccagaagcc attgaaatct acctctcttt cactgtttta taaaaaagtg
2101 tatcggctag cctatctccg gctaaataca ctttgtgaac gccttctgtc tgagcaccca
2161 gaattagaac atatcatctg gacccttttc cagcacaccc tgcagaatga gtatgaactc
2221 atgagagaca ggcatttgga ccaaattatg atgtgttcca tgtatggcat atgcaaagtg
2281 aagaatatag accttaaatt caaaatcatt gtaacagcat acaaggatct tcctcatgct
2341 gttcaggaga cattcaaacg tgttttgatc aaagaagagg agtatgattc tattatagta
2401 ttctataact cggtcttcat gcagagactg aaaacaaata ttttgcagta tgcttccacc
2461 aggcccccta ccttgtcacc aatacctcac attcctcgaa gcccttacaa gtttcctagt
2521 tcacccttac ggattcctgg agggaacatc tatatttcac ccctgaagag tccatataaa
2581 atttcagaag gtctgccaac accaacaaaa atgactccaa gatcaagaat cttagtatca
2641 attggtgaat cattcgggac ttctgagaag ttccagaaaa taaatcagat ggtatgtaac
2701 agcgaccgtg tgctcaaaag aagtgctgaa ggaagcaacc ctcctaaacc actgaaaaaa
2761 ctacgctttg atattgaagg atcagatgaa gcagatggaa gtaaacatct cccaggagag
2821 tccaaatttc agcagaaact ggcagaaatg acttctactc gaacacgaat gcaaaagcag
2881 aaaatgaatg atagcatgga tacctcaaac aaggaagaga aatgaggatc tcaggacctt
2941 ggtggacact gtgtacacct ctggattcat tgtctctcac agatgtgact gtataacttt
3001 cccaggttct gtttatggcc acatttaata tcttcagctc tttttgtgga tataaaatgt
3061 gcagatgcaa ttgtttgggt gagtcctaag ccacttgaaa tgttagtcat tgttatttat
3121 acaagattga aaatcttgtg taaatcctgc catttaaaaa gttgtagcag attgtttcct
3181 cttccaaagt aaaattgctg tgctttatgg atagtaagaa tggccctaga gtgggagtcc
3241 tgataaccca ggcctgtctg actactttgc cttcttttgt agcatatagg tgatgtttgc
3301 tcttgttttt attaatttat atgtatattt ttttaattta acatgaacac ccttagaaaa
3361 tgtgtcctat ctatcttcca aatgcaattt gattgactgc ccattcacca aaattatcct
3421 gaactcttct gcaaaaatgg atattattag aaattagaaa aaaattacta attttacaca
3481 ttagatttta ttttactatt ggaatctgat atactgtgtg cttgttttat aaaattttgc
3541 ttttaattaa ataaaagctg gaagcaaagt ataaccatat gatactatca tactactgaa
3601 acagatttca tacctcagaa tgtaaaagaa cttactgatt attttcttca tccaacttat
3661 gtttttaaat gaggattatt gatagtactc ttggttttta taccattcag atcactgaat
3721 ttataaagta cccatctagt acttgaaaaa gtaaagtgtt ctgccagatc ttaggtatag
3781 aggaccctaa cacagtatat cccaagtgca ctttctaatg tttctgggtc ctgaagaatt
3841 aagatacaaa ttaattttac tccataaaca gactgttaat tataggagcc ttaatttttt
3901 tttcatagag atttgtctaa ttgcatctca aaattattct gccctcctta atttgggaag
3961 gtttgtgttt tctctggaat ggtacatgtc ttccatgtat cttttgaact ggcaattgtc
4021 tatttatctt ttattttttt aagtcagtat ggtctaacac tggcatgttc aaagccacat
4081 tatttctagt ccaaaattac aagtaatcaa gggtcattat gggttaggca ttaatgtttc
4141 tatctgattt tgtgcaaaag cttcaaatta aaacagctgc attagaaaaa gaggcgcttc
4201 tcccctcccc tacacctaaa ggtgtattta aactatcttg tgtgattaac ttatttagag
4261 atgctgtaac ttaaaatagg ggatatttaa ggtagcttca gctagctttt aggaaaatca
4321 ctttgtctaa ctcagaatta tttttaaaaa gaaatctggt cttgttagaa aacaaaattt
4381 tattttgtgc tcatttaagt ttcaaactta ctattttgac agttattttg ataacaatga
4441 cactagaaaa cttgactcca tttcatcatt gtttctgcat gaatatcata caaatcagtt
4501 agtttttagg tcaagggctt actatttctg ggtcttttgc tactaagttc acattagaat
4561 tagtgccaga attttaggaa cttcagagat cgtgtattga gatttcttaa ataatgcttc
4621 agatattatt gctttattgc ttttttgtat tggttaaaac tgtacattta aaattgctat
4681 gttactattt tctacaatta atagtttgtc tattttaaaa taaattagtt gttaagagtc
//