LOCUS HUMGLCB 2191 bp mRNA linear HUM 08-NOV-1994
DEFINITION Human beta-glucuronidase mRNA, complete cds.
ACCESSION M15182
VERSION M15182.1
KEYWORDS alternative splicing; beta-D-glucuronoside glucuronosohydrolase;
beta-glucuronidase; glucuronidase; glucuronohydrolase; hydrolase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2191)
AUTHORS Oshima,A., Kyle,J.W., Miller,R.D., Hoffmann,J.W., Powell,P.P.,
Grubb,J.H., Sly,W.S., Tropak,M., Guise,K.S. and Gravel,R.A.
TITLE Cloning, sequencing, and expression of cDNA for human
beta-glucuronidase
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 84 (3), 685-689 (1987)
PUBMED 3468507
COMMENT Original source text: Human fibroblasts cDNA clone pHUGF and
placenta cDNA clones pHUGP13 and pHUGP15.
Draft entry and copy of computer-readable sequence of [1] kindly
provided by R.D.Miller, 08-MAY-1987.
Four potential N-linked glycosylation sites are located at bases
543-551, 840-848, 1284-1292 and 1917-1925. Potential poly-A
signals were found 25 and 48 bp upstream of the poly-A site at
nucleotides 2139-2144 and 2162-2167 respectively.
Two types of mRNA exit due to alternative splicing. The short form,
lacking bases 939-1091 below, encodes a protein lacking
beta-glucuronidase activity.
FEATURES Location/Qualifiers
source 1..2191
/db_xref="H-InvDB:HIT000194383"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="7q22"
gene 1..2191
/gene="GUSB"
mRNA <1..2191
/gene="GUSB"
/product="glucuronidase-b mRNA"
CDS 27..1982
/gene="GUSB"
/note="beta-glucuronidase precursor (EC 3.2.1.31)"
/codon_start=1
/protein_id="AAA52561.1"
/db_xref="GDB:G00-120-025"
/translation="MARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGL
WSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYE
REVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVG
PLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSV
LLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGT
QGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKS
QFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEV
MQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVAN
EPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWY
HDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLL
EQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRE
RYWKIANETRYPHSVAKSQCLENSPFT"
sig_peptide 27..92
/gene="GUSB"
/note="beta-glucuronidase signal peptide"
mat_peptide 93..1979
/gene="GUSB"
/product="beta-glucuronidase mature peptide"
BASE COUNT 497 a 589 c 630 g 475 t
ORIGIN 33 bp upstream of SmaI site.
1 ggtggccgag cgggggaccg ggaagcatgg cccgggggtc ggcggttgcc tgggcggcgc
61 tcgggccgtt gttgtggggc tgcgcgctgg ggctgcaggg cgggatgctg tacccccagg
121 agagcccgtc gcgggagtgc aaggagctgg acggcctctg gagcttccgc gccgacttct
181 ctgacaaccg acgccggggc ttcgaggagc agtggtaccg gcggccgctg tgggagtcag
241 gccccaccgt ggacatgcca gttccctcca gcttcaatga catcagccag gactggcgtc
301 tgcggcattt tgtcggctgg gtgtggtacg aacgggaggt gatcctgccg gagcgatgga
361 cccaggacct gcgcacaaga gtggtgctga ggattggcag tgcccattcc tatgccatcg
421 tgtgggtgaa tggggtcgac acgctagagc atgagggggg ctacctcccc ttcgaggccg
481 acatcagcaa cctggtccag gtggggcccc tgccctcccg gctccgaatc actatcgcca
541 tcaacaacac actcaccccc accaccctgc caccagggac catccaatac ctgactgaca
601 cctccaagta tcccaagggt tactttgtcc agaacacata ttttgacttt ttcaactacg
661 ctggactgca gcggtctgta cttctgtaca cgacacccac cacctacatc gatgacatca
721 ccgtcaccac cagcgtggag caagacagtg ggctggtgaa ttaccagatc tctgtcaagg
781 gcagtaacct gttcaagttg gaagtgcgtc ttttggatgc agaaaacaaa gtcgtggcga
841 atgggactgg gacccagggc caacttaagg tgccaggtgt cagcctctgg tggccgtacc
901 tgatgcacga acgccctgcc tatctgtatt cattggaggt gcagctgact gcacagacgt
961 cactggggcc tgtgtctgac ttctacacac tccctgtggg gatccgcact gtggctgtca
1021 ccaagagcca gttcctcatc aatgggaaac ctttctattt ccacggtgtc aacaagcatg
1081 aggatgcgga catccgaggg aagggcttcg actggccgct gctggtgaag gacttcaacc
1141 tgcttcgctg gcttggtgcc aacgctttcc gtaccagcca ctacccctat gcagaggaag
1201 tgatgcagat gtgtgaccgc tatgggattg tggtcatcga tgagtgtccc ggcgtgggcc
1261 tggcgctgcc gcagttcttc aacaacgttt ctctgcatca ccacatgcag gtgatggaag
1321 aagtggtgcg tagggacaag aaccaccccg cggtcgtgat gtggtctgtg gccaacgagc
1381 ctgcgtccca cctagaatct gctggctact acttgaagat ggtgatcgct cacaccaaat
1441 ccttggaccc ctcccggcct gtgacctttg tgagcaactc taactatgca gcagacaagg
1501 gggctccgta tgtggatgtg atctgtttga acagctacta ctcttggtat cacgactacg
1561 ggcacctgga gttgattcag ctgcagctgg ccacccagtt tgagaactgg tataagaagt
1621 atcagaagcc cattattcag agcgagtatg gagcagaaac gattgcaggg tttcaccagg
1681 atccacctct gatgttcact gaagagtacc agaaaagtct gctagagcag taccatctgg
1741 gtctggatca aaaacgcaga aaatatgtgg ttggagagct catttggaat tttgccgatt
1801 tcatgactga acagtcaccg acgagagtgc tggggaataa aaaggggatc ttcactcggc
1861 agagacaacc aaaaagtgca gcgttccttt tgcgagagag atactggaag attgccaatg
1921 aaaccaggta tccccactca gtagccaagt cacaatgttt ggaaaacagc ccgtttactt
1981 gagcaagact gataccacct gcgtgtccct tcctccccga gtcagggcga cttccacagc
2041 agcagaacaa gtgcctcctg gactgttcac ggcagaccag aacgtttctg gcctgggttt
2101 tgtggtcatc tattctagca gggaacacta aaggtggaaa taaaagattt tctattatgg
2161 aaataaagag ttggcatgaa agtcgctact g
//