LOCUS HUMTSPA 1250 bp mRNA linear HUM 14-JAN-1995
DEFINITION Human thrombospondin (TSP) mRNA, 5' end.
ACCESSION M14326
VERSION M14326.1
KEYWORDS binding site; glycoprotein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1250)
AUTHORS Dixit,V.M., Hennessy,S.W., Grant,G.A., Rotwein,P. and Frazier,W.A.
TITLE Characterization of a cDNA encoding the heparin and collagen
binding domains of human thrombospondin
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (15), 5449-5453 (1986)
PUBMED 3461443
REFERENCE 2 (sites)
AUTHORS Burglin,T.R. and Barnes,T.M.
TITLE Introns in sequence tags
JOURNAL Nature 357 (6377), 367-368 (1992)
PUBMED 1350660
COMMENT Original source text: Human fibroblast cell line JY10, cDNA to
mRNA, clones M13mp18 and M13mp19.
FEATURES Location/Qualifiers
source 1..1250
/db_xref="H-InvDB:HIT000194328"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="15q15"
gene 1..1250
/gene="THBS1"
CDS 121..>1250
/gene="THBS1"
/note="Thrombospondin"
/codon_start=1
/protein_id="AAA61237.1"
/db_xref="GDB:G00-120-438"
/translation="MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGS
GRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRG
TLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFV
QEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFV
FGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDE
LSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVD
SCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPRC"
sig_peptide 121..174
/gene="THBS1"
/note="thrombospondin signal peptide"
mat_peptide 175..>1250
/gene="THBS1"
/product="thrombospondin mature peptide"
BASE COUNT 278 a 388 c 348 g 236 t
ORIGIN 65 bp upstream of SmaI site.
1 ccccccccgg acgcacaggc attccccgcg cccctccagc cctcgccgcc ctcgccaccg
61 ctcccgggcc gccgcgctcc ggtacacaca ggatccctgc tgggcaccaa cagctccacc
121 atggggctgg cctggggact aggcgtcctg ttcctgatgc atgtgtgtgg caccaaccgc
181 attccagagt ctggcggaga caacagcgtg tttgacatct ttgaactcac cggggccgcc
241 cgcaaggggt ctgggcgccg actggtgaag ggccccgacc cttccagccc agctttccgc
301 atcgaggatg ccaacctgat cccccctgtg cctgatgaca agttccaaga cctggtggat
361 gctgtgcggg cagaaaaggg tttcctcctt ctggcatccc tgaggcagat gaagaagacc
421 cggggcacgc tgctggccct ggagcggaaa gaccactctg gccaggtctt cagcgtggtg
481 tccaatggca aggcgggcac cctggacctc agcctgaccg tccaaggaaa gcagcacgtg
541 gtgtctgtgg aagaagctct cctggcaacc ggccagtgga agagcatcac cctgtttgtg
601 caggaagaca gggcccagct gtacatcgac tgtgaaaaga tggagaatgc tgagttggac
661 gtccccatcc aaagcgtctt caccagagac ctggccagca tcgccagact ccgcatcgca
721 aaggggggcg tcaatgacaa tttccagggg gtgctgcaga atgtgaggtt tgtctttgga
781 accacaccag aagacatcct caggaacaaa ggctgctcca gctctaccag tgtcctcctc
841 acccttgaca acaacgtggt gaatggttcc agccctgcca tccgcactaa ctacattggc
901 cacaagacaa aggacttgca agccatctgc ggcatctcct gtgatgagct gtccagcatg
961 gtcctggaac tcaggggcct gcgcaccatt gtgaccacgc tgcaggacag catccgcaaa
1021 gtgactgaag agaacaaaga gttggccaat gagctgaggc ggcctcccct atgctatcac
1081 aacggagttc agtacagaaa taacgaggaa tggactgttg atagctgcac tgagtgtcac
1141 tgtcagaact cagttaccat ctgcaaaaag gtgtcctgcc ccatcatgcc ctgctccaat
1201 gccacagttc ctgatggaga atgctgtcct cgctgttggc ctagatgtga
//