LOCUS       HUMAPOBB               13993 bp    mRNA    linear   HUM 22-JUL-1996
DEFINITION  Human apolipoprotein B-100 mRNA, complete cds.
ACCESSION   M14162
VERSION     M14162.1
KEYWORDS    apolipoprotein B100.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (sites)
  AUTHORS   Law,S.W., Grant,S.M., Higuchi,K., Hospattankar,A., Lackner,K.,
            Lee,N. and Brewer,H.B. Jr.
  TITLE     Human liver apolipoprotein B-100 cDNA: complete nucleic acid and
            derived amino acid sequence
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 83 (21), 8142-8146 (1986)
   PUBMED   3464946
REFERENCE   2  (sites)
  AUTHORS   Soria,L.F., Ludwig,E.H., Clarke,H.R., Vega,G.L., Grundy,S.M. and
            McCarthy,B.J.
  TITLE     Association between a specific apolipoprotein B mutation and
            familial defective apolipoprotein B-100
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86 (2), 587-591 (1989)
   PUBMED   2563166
COMMENT     Original source text: Homo sapiens (clone:  lambda-MDB-[1-4],
            pMDB[5-10].) liver cDNA to mRNA.
            Draft entry and clean copy sequence for [1] kindly provided by
            S.W.Law, 22-JAN-1987.
            The mutation at position 10580 results in the substitution of a
            glutamine for an arginine.  This causes hypercholesterolemia
            because of a defective low density lipoprotein [2].
FEATURES             Location/Qualifiers
     source          1..13993
                     /db_xref="H-InvDB:HIT000194303"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /map="2p24-p23"
                     /clone="lambda-MDB-[1-4], pMDB[5-10]."
                     /tissue_type="liver"
     gene            1..13993
                     /gene="APOB"
     mRNA            <1..13993
                     /gene="APOB"
                     /product="apoB mRNA"
     CDS             1..13692
                     /gene="APOB"
                     /note="apolipoprotein B-100 precursor"
                     /codon_start=1
                     /protein_id="AAB04636.1"
                     /db_xref="GDB:G00-119-686"
                     /translation="MDPPRPALLALLALPALLLLLLAGARAEEEMLENVSLVCPKDAT
                     RFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEV
                     YGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGI
                     ISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPI
                     RTGISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNKY
                     GMVAQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKK
                     LTISEQNIQRANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQALVQCGQPQCST
                     HILQWLKRVHANPLLIDVVTYLVALIPEPSAQQLREIFNMARDQRSRATLYALSHAVN
                     NYHKTNPTGTQELLDIANYLMEQIQDDCTGDEDYTYLILRVIGNMGQTMEQLTPELKS
                     SILKCVQSTKPSLMIQKAAIQALRKMEPKDKDQEVLLQTFLDDASPGDKRLAAYLMLM
                     RSPSQADINKIVQILPWEQNEQVKNFVASHIANILNSEELDIQDLKKLVKEALKESQL
                     PTVMDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGF
                     ASADLIEIGLEGKGFEPTLEAPFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHFGY
                     TKDDKHEQDMVNGIMLSVEKLIKDLKSKEVPEARAYLRILGEELGFASLHDLRLLGKL
                     LLMGARTLQGIPQMIGEVIRKGSKNDFFLHYIFMENAFELPTGAGLQLQISSSGVIAP
                     GAKAGVKLEVANMQAELVAKPSVSVEFVTNMGIIIPDFARSGVQMNTNFFHESGLEAH
                     VALKAGKLKFIIPSPKRPVKLLSGGNTLHLVSTTKTEVIPPLIENRQSWSVCKQVFPG
                     LNYCTSGAYSNASSTDSASYYPLTGDTRLELELRPTGEIEQYSVSATYELQREDRALV
                     DTLKFVTQAEGAKQTEATMTFKYNRQSMTLSSEVQIPDFDVDLGTILRVNDESTEGKT
                     SYRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIPRLQAEARSEILAHWSPAKL
                     LLQMDSSATAYGSTVSKRVAWHYDEEKIEFEWNTGTNVDTKKMTSNFPVDLSDYPKSL
                     HMYANRLLDHRVPQTDMTFRHVGSKLIVAMSSWLQKASGSLPYTQTLQDHLNSLKEFN
                     LQNMGLPDSHIPENLFLKSDGRVKYTLNKNSLKIEIPLPFGGKSSRDLKMLETVRTPA
                     LHFKSVGFHLPSREFQVPTFTIPKLYQLQVPLLGVLDLSTNVYSNLYNWSASYSGGNT
                     STDHFSLRARYHMKADSVVDLLSYNVQGSGETTYDHKNTFTLSCDGSLRHKFLDSNIK
                     FSHVEKLGNNPVSKGLLIFDASSSWGPQMSASVHLDSKKKQHLFVKEVKIDGQFRVSS
                     FYAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGTNQITGRYEDGTLSLTSTSDLQS
                     GIIKNTASLKYENYELTLKSDTNGKYKNFATSNKMDMTFSKQNALLRSEYQADYESLR
                     FFSLLSGSLNSHGLELNADILGTDKINSGAHKATLRIGQDGISTSATTNLKCSLLVLE
                     NELNAELGLSGASMKLTTNGRFREHNAKFSLDGKAALTELSLGSAYQAMILGVDSKNI
                     FNFKVSQEGLKLSNDMMGSYAEMKFDHTNSLNIAGLSLDFSSKLDNIYSSDKFYKQTV
                     NLQLQPYSLVTTLNSDLKYNALDLTNNGKLRLEPLKLHVAGNLKGAYQNNEIKHIYAI
                     SSAALSASYKADTVAKVQGVEFSHGLNTDIAGLASAIDMSTNYNSDSLHFSNVFRSVM
                     APFTMTIDAHTNGNGKLALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRK
                     SISAALEHKVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLAD
                     LTLLDSPIKVPLLLSEPININDALEMRDAVEKPQEFTIVAFVKYDKNQDVHSINLPFF
                     ETLQEYFERNRQTIIVVLENVQRNLKHINIDQFVRKYRAALGKLPQQANDYLNSFNWE
                     RQVSHAKEKLTALTKKYRITENDIQIALDDAKINFNEKLSQLQTYMIQFDQYIKDSYD
                     LHDLKIAIANIIDEIIEKLKSLDEHYHTRVNLVKTIHDLHLFIENIDFNKSGSSTASW
                     IQNVDTKYQIRIQIQEKLQQLKRHIQNIDIQHLAGKLKQHIEAIDVRVLLDQLGTTIS
                     FERINDVLEHVKHFVINLIGDFEVAEKINAFRAKVHELIERYEVDQQIQVLMDKLVEL
                     AHQYKLKETIQKLSNVLQQVKIKDYFEKLVGFIDDAVKKLNELSFKTFIEDVNKFLDM
                     LIKKLKSFDYHQFVDETNDKIREVTQRLNGEIQALELPQKAEALKLFLEETKATVAVY
                     LESLQDTKITLIINWLQEALSSASLAHMKAKFRETLEDTRDRMYQMDIQQELQRYLSL
                     VGQVYSTLVTYISDWWTLAAKNLTDFAEQYSIQDWAKRMKALVEQGFTVPEIKTILGT
                     MPAFEVSLQALQKATFQTPDFIVPLTDLRIPSVQINFKDLKNIKIPSRFSTPEFTILN
                     TFHIPSFTIDFVEMKVKIIRTIDQMLNSELQWPVPDIYLRDLKVEDIPLARITLPDFR
                     LPEIAIPEFIIPTLNLNDFQVPDLHIPEFQLPHISHTIEVPTFGKLYSILKIQSPLFT
                     LDANADIGNGTTSANEAGIAASITAKGESKLEVLNFDFQANAQLSNPKINPLALKESV
                     KFSSKYLRTEHGSEMLFFGNAIEGKSNTVASLHTEKNTLELSNGVIVKINNQLTLDSN
                     TKYFHKLNIPKLDFSSQADLRNEIKTLLKAGHIAWTSSGKGSWKWASPRFSDEGTHES
                     QISFTIEGPLTSFGLSNKINSKHLRVNQNLVYESGSLNFSKLEIQSQVDSQHVGHSVL
                     TAKGMALFGEGKAEFTGRHDAHLNGKVIGTLKNSLFFSAQPFEITASTNNEGNLKVRF
                     PLRLTGKIDFLNNYALFLSPSAQQASWQVSARFNQYKYNQNFSAGNNENIMEAHVGIN
                     GEANLDFLNIPLTIPEMRLPYTIITTPPLKDFSLWEKTGLKEFLKTTKQSFDLSVKAQ
                     YKKNKHRHSITNPLAVLCEFISQSIKSFDRHFEKNRNNALDFVTKSYNETKIKFDKYK
                     AEKSHDELPRTFQIPGYTVPVVNVEVSPFTIEMSAFGYVFPKAVSMPSFSIIGSDVRV
                     PSYTLILPSLELPVLHVPRNLKLSLPDFKELCTISHIFIPAMGNITYDFSFKSSVITL
                     NTNAELFNQSDIVAHLLSSSSSVIDALQYKLEGTTRLTRKRGLKLATALSLSNKFVEG
                     SHNSTVSLTTKNMEVSVAKTTKPEIPILRMNFKQELNGNTKSKPTVSSSMEFKYDFNS
                     SMLYSTAKGAVDHKLSLESLTSYFSIESSTKGDVKGSVLSREYSGTIASEANTYLNSK
                     STRSSVKLQGTSKIDDIWNLEVKENFAGEATLQRIYSLWEHSTKNHLQLEGLFFTNGE
                     HTSKATLELSPWQMSALVQVHASQPSSFHDFPDLGQEVALNANTKNQKIRWKNEVRIH
                     SGSFQSQVELSNDQEKAHLDIAGSLEGHLRFLKNIILPVYDKSLWDFLKLDVTTSIGR
                     RQHLRVSTAFVYTKNPNGYSFSIPVKVLADKFIIPGLKLNDLNSVLVMPTFHVPFTDL
                     QVPSCKLDFREIQIYKKLRTSSFALTLPTLPEVKFPEVDVLTKYSQPEDSLIPFFEIT
                     VPESQLTVSQFTLPKSVSDGIAALDLNAVANKIADFELPTIIVPEQTIEIPSIKFSVP
                     AGIVIPSFQALTARFEVDSPVYNATWSASLKNKADYVETVLDSTCSSTVQFLEYELNV
                     LGTHKIEDGTLASKTKGTLAHRDFSAEYEEDGKYEGLQEWEGKAHLNIKSPAFTDLHL
                     RYQKDKKGISTSAASPAVGTVGMDMDEDDDFSKWNFYYSPQSSPDKKLTIFKTELRVR
                     ESDEETQIKVNWEEEAASGLLTSLKDNVPKATGVLYDYVNKYHWEHTGLTLREVSSKL
                     RRNLQNNAEWVYQGAIRQIDDIDVRFQKAASGTTGTYQEWKDKAQNLYQELLTQEGQA
                     SFQGLKDNVFDGLVRVTQKFHMKVKKLIDSLIDFLNFPRFQFPGKPGIYTREELCTMF
                     MREVGTVLSQVYSKVHNGSEILFSYFQDLVITLPFELRKHKLIDVISMYRELLKDLSK
                     EAQEVFKAIQSLKTTEVLRNLQDLLQFIFQLIEDNIKQLKEMKFTYLINYIQDEINTI
                     FNDYIPYVFKLLKENLCLNLHKFNEFIQNELQEASQELQQIHQYIMALREEYFDPSIV
                     GWTVKYYELEEKIVSLIKNLLVALKDFHSEYIVSASNFTSQLSSQVEQFLHRNIQEYL
                     SILTDPDGKGKEKIAELSATAQEIIKSQAIATKKIISDYHQQFRYKLQDFSDQLSDYY
                     EKFIAESKRLIDLSIQNYHTFLIYITELLKKLQSTTVMNPYMKLAPGELTIIL"
     sig_peptide     1..81
                     /gene="APOB"
                     /note="apolipoprotein B-100 signal peptide"
     mat_peptide     82..13689
                     /gene="APOB"
                     /product="apolipoprotein B-100"
     variation       10580
                     /gene="APOB"
                     /note="g in wt; a in defective low density lipoprotein
                     [2]"
BASE COUNT         4352 a         3191 c         2860 g         3590 t
ORIGIN      392 bp upstream of SacI site.
        1 atggacccgc cgaggcccgc gctgctggcg ctgctggcgc tgcctgcgct gctgctgctg
       61 ctgctggcgg gcgccagggc cgaagaggaa atgctggaaa atgtcagcct ggtctgtcca
      121 aaagatgcga cccgattcaa gcacctccgg aagtacacat acaactatga ggctgagagt
      181 tccagtggag tccctgggac tgctgattca agaagtgcca ccaggatcaa ctgcaaggtt
      241 gagctggagg ttccccagct ctgcagcttc atcctgaaga ccagccagtg caccctgaaa
      301 gaggtgtatg gcttcaaccc tgagggcaaa gccttgctga agaaaaccaa gaactctgag
      361 gagtttgctg cagccatgtc caggtatgag ctcaagctgg ccattccaga agggaagcag
      421 gttttccttt acccggagaa agatgaacct acttacatcc tgaacatcaa gaggggcatc
      481 atttctgccc tcctggttcc cccagagaca gaagaagcca agcaagtgtt gtttctggat
      541 accgtgtatg gaaactgctc cactcacttt accgtcaaga cgaggaaggg caatgtggca
      601 acagaaatat ccactgaaag agacctgggg cagtgtgatc gcttcaagcc catccgcaca
      661 ggcatcagcc cacttgctct catcaaaggc atgacccgcc ccttgtcaac tctgatcagc
      721 agcagccagt cctgtcagta cacactggac gctaagagga agcatgtggc agaagccatc
      781 tgcaaggagc aacacctctt cctgcctttc tcctacaaga ataagtatgg gatggtagca
      841 caagtgacac agactttgaa acttgaagac acaccaaaga tcaacagccg cttctttggt
      901 gaaggtacta agaagatggg cctcgcattt gagagcacca aatccacatc acctccaaag
      961 caggccgaag ctgttttgaa gactctccag gaactgaaaa aactaaccat ctctgagcaa
     1021 aatatccaga gagctaatct cttcaataag ctggttactg agctgagagg cctcagtgat
     1081 gaagcagtca catctctctt gccacagctg attgaggtgt ccagccccat cactttacaa
     1141 gccttggttc agtgtggaca gcctcagtgc tccactcaca tcctccagtg gctgaaacgt
     1201 gtgcatgcca acccccttct gatagatgtg gtcacctacc tggtggccct gatccccgag
     1261 ccctcagcac agcagctgcg agagatcttc aacatggcga gggatcagcg cagccgagcc
     1321 accttgtatg cgctgagcca cgcggtcaac aactatcata agacaaaccc tacagggacc
     1381 caggagctgc tggacattgc taattacctg atggaacaga ttcaagatga ctgcactggg
     1441 gatgaagatt acacctattt gattctgcgg gtcattggaa atatgggcca aaccatggag
     1501 cagttaactc cagaactcaa gtcttcaatc ctgaaatgtg tccaaagtac aaagccatca
     1561 ctgatgatcc agaaagctgc catccaggct ctgcggaaaa tggagcctaa agacaaggac
     1621 caggaggttc ttcttcagac tttccttgat gatgcttctc cgggagataa gcgactggct
     1681 gcctatctta tgttgatgag gagtccttca caggcagata ttaacaaaat tgtccaaatt
     1741 ctaccatggg aacagaatga gcaagtgaag aactttgtgg cttcccatat tgccaatatc
     1801 ttgaactcag aagaattgga tatccaagat ctgaaaaagt tagtgaaaga agctctgaaa
     1861 gaatctcaac ttccaactgt catggacttc agaaaattct ctcggaacta tcaactctac
     1921 aaatctgttt ctcttccatc acttgaccca gcctcagcca aaatagaagg gaatcttata
     1981 tttgatccaa ataactacct tcctaaagaa agcatgctga aaactaccct cactgccttt
     2041 ggatttgctt cagctgacct catcgagatt ggcttggaag gaaaaggctt tgagccaaca
     2101 ttggaggctc cttttgggaa gcaaggattt ttcccagaca gtgtcaacaa agctttgtac
     2161 tgggttaatg gtcaagttcc tgatggtgtc tctaaggtct tagtggacca ctttggctat
     2221 accaaagatg ataaacatga gcaggatatg gtaaatggaa taatgctcag tgttgagaag
     2281 ctgattaaag atttgaaatc caaagaagtc ccggaagcca gagcctacct ccgcatcttg
     2341 ggagaggagc ttggttttgc cagtctccat gacctccgac tcctgggaaa gctgcttctg
     2401 atgggtgccc gcactctgca ggggatcccc cagatgattg gagaggtcat caggaagggc
     2461 tcaaagaatg acttttttct tcactacatc ttcatggaga atgcctttga actccccact
     2521 ggagctggat tacagttgca aatatcttca tctggagtca ttgctcccgg agccaaggct
     2581 ggagtaaaac tggaagtagc caacatgcag gctgaactgg tggcaaaacc ctccgtgtct
     2641 gtggagtttg tgacaaatat gggcatcatc attccggact tcgctaggag tggggtccag
     2701 atgaacacca acttcttcca cgagtcgggt ctggaggctc atgttgccct aaaagctggg
     2761 aagctgaagt ttatcattcc ttccccaaag agaccagtca agctgctcag tggaggcaac
     2821 acattacatt tggtctctac caccaaaacg gaggtcatcc cacctctcat tgagaacagg
     2881 cagtcctggt cagtttgcaa gcaagtcttt cctggcctga attactgcac ctcaggcgct
     2941 tactccaacg ccagctccac agactccgcc tcctactatc cgctgaccgg ggacaccaga
     3001 ttagagctgg aactgaggcc tacaggagag attgagcagt attctgtcag cgcaacctat
     3061 gagctccaga gagaggacag agccttggtg gataccctga agtttgtaac tcaagcagaa
     3121 ggcgcgaagc agactgaggc taccatgaca ttcaaatata atcggcagag tatgaccttg
     3181 tccagtgaag tccaaattcc ggattttgat gttgacctcg gaacaatcct cagagttaat
     3241 gatgaatcta ctgagggcaa aacgtcttac agactcaccc tggacattca gaacaagaaa
     3301 attactgagg tcgccctcat gggccaccta agttgtgaca caaaggaaga aagaaaaatc
     3361 aagggtgtta tttccatacc ccgtttgcaa gcagaagcca gaagtgagat cctcgcccac
     3421 tggtcgcctg ccaaactgct tctccaaatg gactcatctg ctacagctta tggctccaca
     3481 gtttccaaga gggtggcatg gcattatgat gaagagaaga ttgaatttga atggaacaca
     3541 ggcaccaatg tagataccaa aaaaatgact tccaatttcc ctgtggatct ctccgattat
     3601 cctaagagct tgcatatgta tgctaataga ctcctggatc acagagtccc tcaaacagac
     3661 atgactttcc ggcacgtggg ttccaaatta atagttgcaa tgagctcatg gcttcagaag
     3721 gcatctggga gtcttcctta tacccagact ttgcaagacc acctcaatag cctgaaggag
     3781 ttcaacctcc agaacatggg attgccagac tcccacatcc cagaaaacct cttcttaaaa
     3841 agcgatggcc gcgtcaaata taccttgaac aagaacagtt tgaaaattga gattcctttg
     3901 ccttttggtg gcaaatcctc cagagatcta aagatgttag agactgttag gacaccagcc
     3961 ctccacttca agtctgtggg attccatctg ccatctcgag agttccaagt ccctactttt
     4021 accattccca agttgtatca actgcaagtg cctctcctgg gtgttctaga cctctccacg
     4081 aatgtctaca gcaacttgta caactggtcc gcctcctaca gtggtggcaa caccagcaca
     4141 gaccatttca gccttcgggc tcgttaccac atgaaggctg actctgtggt tgacctgctt
     4201 tcctacaatg tgcaaggatc tggagaaaca acatatgacc acaagaatac gttcacacta
     4261 tcatgtgatg ggtctctacg ccacaaattt ctagattcga atatcaaatt cagtcatgta
     4321 gaaaaacttg gaaacaaccc agtctcaaaa ggtttactaa tattcgatgc atctagttcc
     4381 tggggaccac agatgtctgc ttcagttcat ttggactcca aaaagaaaca gcatttgttt
     4441 gtcaaagaag tcaagattga tgggcagttc agagtctctt cgttctatgc taaaggcaca
     4501 tatggcctgt cttgtcagag ggatcctaac actggccggc tcaatggaga gtccaacctg
     4561 aggtttaact cctcctacct ccaaggcacc aaccagataa caggaagata tgaagatgga
     4621 accctctccc tcacctccac ctctgatctg caaagtggca tcattaaaaa tactgcttcc
     4681 ctaaagtatg agaactacga gctgacttta aaatctgaca ccaatgggaa gtataagaac
     4741 tttgccactt ctaacaagat ggatatgacc ttctctaagc aaaatgcact gctgcgttct
     4801 gaatatcagg ctgattacga gtcattgagg ttcttcagcc tgctttctgg atcactaaat
     4861 tcccatggtc ttgagttaaa tgctgacatc ttaggcactg acaaaattaa tagtggtgct
     4921 cacaaggcga cactaaggat tggccaagat ggaatatcta ccagtgcaac gaccaacttg
     4981 aagtgtagtc tcctggtgct ggagaatgag ctgaatgcag agcttggcct ctctggggca
     5041 tctatgaaat taacaacaaa tggccgcttc agggaacaca atgcaaaatt cagtctggat
     5101 gggaaagccg ccctcacaga gctatcactg ggaagtgctt atcaggccat gattctgggt
     5161 gtcgacagca aaaacatttt caacttcaag gtcagtcaag aaggacttaa gctctcaaat
     5221 gacatgatgg gctcatatgc tgaaatgaaa tttgaccaca caaacagtct gaacattgca
     5281 ggcttatcac tggacttctc ttcaaaactt gacaacattt acagctctga caagttttat
     5341 aagcaaactg ttaatttaca gctacagccc tattctctgg taactacttt aaacagtgac
     5401 ctgaaataca atgctctgga tctcaccaac aatgggaaac tacggctaga acccctgaag
     5461 ctgcatgtgg ctggtaacct aaaaggagcc taccaaaata atgaaataaa acacatctat
     5521 gccatctctt ctgctgcctt atcagcaagc tataaagcag acactgttgc taaggttcag
     5581 ggtgtggagt ttagccatgg gctcaacaca gacatcgctg ggctggcttc agccattgac
     5641 atgagcacaa actataattc agactcactg catttcagca atgtcttccg ttctgtaatg
     5701 gccccgttta ccatgaccat cgatgcacat acaaatggca atgggaaact cgctctctgg
     5761 ggagaacata ctgggcagct gtatagcaaa ttcctgttga aagcagaacc tctggcattt
     5821 actttctctc atgattacaa aggctccaca agtcatcatc tcgtgtctag gaaaagcatc
     5881 agtgcagctc ttgaacacaa agtcagtgcc ctgcttactc cagctgagca gacaggcacc
     5941 tggaaactca agacccaatt taacaacaat gaatacagcc aggacttgga tgcttacaac
     6001 actaaagata aaattggcgt ggagcttact ggacgaactc tggctgacct aactctacta
     6061 gactccccaa ttaaagtgcc acttttactc agtgagccca tcaatatcaa tgatgcttta
     6121 gagatgagag atgccgttga gaagccccaa gaatttacaa ttgttgcttt tgtaaagtat
     6181 gataaaaacc aagatgttca ctccattaac ctcccatttt ttgagacctt gcaagaatat
     6241 tttgagagga atcgacaaac cattatagtt gtactggaaa acgtacagag aaacctgaag
     6301 cacatcaata ttgatcaatt tgtaagaaaa tacagagcag ccctgggaaa actcccacag
     6361 caagctaatg attatctgaa ttcattcaat tgggagagac aagtttcaca tgccaaggag
     6421 aaactgactg ctctcacaaa aaagtataga attacagaaa atgatataca aattgcatta
     6481 gatgatgcca aaatcaactt taatgaaaaa ctatctcaac tgcagacata tatgatacaa
     6541 tttgatcagt atattaaaga tagttatgat ttacatgatt tgaaaatagc tattgctaat
     6601 attattgatg aaatcattga aaaattaaaa agtcttgatg agcactatca tacccgtgta
     6661 aatttagtaa aaacaatcca tgatctacat ttgtttattg aaaatattga ttttaacaaa
     6721 agtggaagta gtactgcatc ctggattcaa aatgtggata ctaagtacca aatcagaatc
     6781 cagatacaag aaaaactgca gcagcttaag agacacatac agaatataga catccagcac
     6841 ctagctggaa agttaaaaca acacattgag gctattgatg ttagagtgct tttagatcaa
     6901 ttgggaacta caatttcatt tgaaagaata aatgatgttc ttgagcatgt caaacacttt
     6961 gttataaatc ttattgggga ttttgaagta gctgagaaaa tcaatgcctt cagagccaaa
     7021 gtccatgagt taatcgagag gtatgaagta gaccaacaaa tccaggtttt aatggataaa
     7081 ttagtagagt tggcccacca atacaagttg aaggagacta ttcagaagct aagcaatgtc
     7141 ctacaacaag ttaagataaa agattacttt gagaaattgg ttggatttat tgatgatgct
     7201 gtcaagaagc ttaatgaatt atcttttaaa acattcattg aagatgttaa caaattcctt
     7261 gacatgttga taaagaaatt aaagtcattt gattaccacc agtttgtaga tgaaaccaat
     7321 gacaaaatcc gtgaggtgac tcagagactc aatggtgaaa ttcaggctct ggaactacca
     7381 caaaaagctg aagcattaaa actgttttta gaggaaacca aggccacagt tgcagtgtat
     7441 ctggaaagcc tacaggacac caaaataacc ttaatcatca attggttaca ggaggcttta
     7501 agttcagcat ctttggctca catgaaggcc aaattccgag agactctaga agatacacga
     7561 gaccgaatgt atcaaatgga cattcagcag gaacttcaac gatacctgtc tctggtaggc
     7621 caggtttata gcacacttgt cacctacatt tctgattggt ggactcttgc tgctaagaac
     7681 cttactgact ttgcagagca atattctatc caagattggg ctaaacgtat gaaagcattg
     7741 gtagagcaag ggttcactgt tcctgaaatc aagaccatcc ttgggaccat gcctgccttt
     7801 gaagtcagtc ttcaggctct tcagaaagct accttccaga cacctgattt tatagtcccc
     7861 ctaacagatt tgaggattcc atcagttcag ataaacttca aagacttaaa aaatataaaa
     7921 atcccatcca ggttttccac accagaattt accatcctta acaccttcca cattccttcc
     7981 tttacaattg actttgtaga aatgaaagta aagatcatca gaaccattga ccagatgctg
     8041 aacagtgagc tgcagtggcc cgttccagat atatatctca gggatctgaa ggtggaggac
     8101 attcctctag cgagaatcac cctgccagac ttccgtttac cagaaatcgc aattccagaa
     8161 ttcataatcc caactctcaa ccttaatgat tttcaagttc ctgaccttca cataccagaa
     8221 ttccagcttc cccacatctc acacacaatt gaagtaccta cttttggcaa gctatacagt
     8281 attctgaaaa tccaatctcc tcttttcaca ttagatgcaa atgctgacat agggaatgga
     8341 accacctcag caaacgaagc aggtatcgca gcttccatca ctgccaaagg agagtccaaa
     8401 ttagaagttc tcaattttga ttttcaagca aatgcacaac tctcaaaccc taagattaat
     8461 ccgctggctc tgaaggagtc agtgaagttc tccagcaagt acctgagaac ggagcatggg
     8521 agtgaaatgc tgttttttgg aaatgctatt gagggaaaat caaacacagt ggcaagttta
     8581 cacacagaaa aaaatacact ggagcttagt aatggagtga ttgtcaagat aaacaatcag
     8641 cttaccctgg atagcaacac taaatacttc cacaaattga acatccccaa actggacttc
     8701 tctagtcagg ctgacctgcg caacgagatc aagacactgt tgaaagctgg ccacatagca
     8761 tggacttctt ctggaaaagg gtcatggaaa tgggcctcgc ccagattctc agatgaggga
     8821 acacatgaat cacaaattag tttcaccata gaaggacccc tcacttcctt tggactgtcc
     8881 aataagatca atagcaaaca cctaagagta aaccaaaact tggtttatga atctggctcc
     8941 ctcaactttt ctaaacttga aattcaatca caagtcgatt cccagcatgt gggccacagt
     9001 gttctaactg ctaaaggcat ggcactgttt ggagaaggga aggcagagtt tactgggagg
     9061 catgatgctc atttaaatgg aaaggttatt ggaactttga aaaattctct tttcttttca
     9121 gcccagccat ttgagatcac ggcatccaca aacaatgaag ggaatttgaa agttcgtttt
     9181 ccattaaggt taacagggaa gatagacttc ctgaataact atgcactgtt tctgagtccc
     9241 agtgcccagc aagcaagttg gcaagtaagt gctaggttca atcagtataa gtacaaccaa
     9301 aatttctctg ctggaaacaa cgagaacatt atggaggccc atgtaggaat aaatggagaa
     9361 gcaaatctgg atttcttaaa cattccttta acaattcctg aaatgcgtct accttacaca
     9421 ataatcacaa ctcctccact gaaagatttc tctctatggg aaaaaacagg cttgaaggaa
     9481 ttcttgaaaa cgacaaagca atcatttgat ttaagtgtaa aagctcagta taagaaaaac
     9541 aaacacaggc attccatcac aaatcctttg gctgtgcttt gtgagtttat cagtcagagc
     9601 atcaaatcct ttgacaggca ttttgaaaaa aacagaaaca atgcattaga ttttgtcacc
     9661 aaatcctata atgaaacaaa aattaagttt gataagtaca aagctgaaaa atctcacgac
     9721 gagctcccca ggacctttca aattcctgga tacactgttc cagttgtcaa tgttgaagtg
     9781 tctccattca ccatagagat gtcggcattc ggctatgtgt tcccaaaagc agtcagcatg
     9841 cctagtttct ccatcatagg ttctgacgtc cgtgtgcctt catacacatt aatcctgcca
     9901 tcattagagc tgccagtcct tcatgtccct agaaatctca agctttctct tccagatttc
     9961 aaggaattgt gtaccataag ccatattttt attcctgcca tgggcaatat tacctatgat
    10021 ttctccttta aatcaagtgt catcacactg aataccaatg ctgaactttt taaccagtca
    10081 gatattgttg ctcatctcct ttcttcatct tcatctgtca ttgatgcact gcagtacaaa
    10141 ttagagggca ccacaagatt gacaagaaaa aggggattga agttagccac agctctgtct
    10201 ctgagcaaca aatttgtgga gggtagtcat aacagtactg tgagcttaac cacgaaaaat
    10261 atggaagtgt cagtggcaaa aaccacaaaa ccggaaattc caattttgag aatgaatttc
    10321 aagcaagaac ttaatggaaa taccaagtca aaacctactg tctcttcctc catggaattt
    10381 aagtatgatt tcaattcttc aatgctgtac tctaccgcta aaggagcagt tgaccacaag
    10441 cttagcttgg aaagcctcac ctcttacttt tccattgagt catctaccaa aggagatgtc
    10501 aagggttcgg ttctttctcg ggaatattca ggaactattg ctagtgaggc caacacttac
    10561 ttgaattcca agagcacacg gtcttcagtg aagctgcagg gcacttccaa aattgatgat
    10621 atctggaacc ttgaagtaaa agaaaatttt gctggagaag ccacactcca acgcatatat
    10681 tccctctggg agcacagtac gaaaaaccac ttacagctag agggcctctt tttcaccaac
    10741 ggagaacata caagcaaagc caccctggaa ctctctccat ggcaaatgtc agctcttgtt
    10801 caggtccatg caagtcagcc cagttccttc catgatttcc ctgaccttgg ccaggaagtg
    10861 gccctgaatg ctaacactaa gaaccagaag atcagatgga aaaatgaagt ccggattcat
    10921 tctgggtctt tccagagcca ggtcgagctt tccaatgacc aagaaaaggc acaccttgac
    10981 attgcaggat ccttagaagg acacctaagg ttcctcaaaa atatcatcct accagtctat
    11041 gacaagagct tatgggattt cctaaagctg gatgtcacca ccagcattgg taggagacag
    11101 catcttcgtg tttcaactgc ctttgtgtac accaaaaacc ccaatggcta ttcattctcc
    11161 atccctgtaa aagttttggc tgataaattc attattcctg ggctgaaact aaatgatcta
    11221 aattcagttc ttgtcatgcc tacgttccat gtcccattta cagatcttca ggttccatcg
    11281 tgcaaacttg acttcagaga aatacaaatc tataagaagc tgagaacttc atcatttgcc
    11341 ctcaccctac caacactccc cgaggtaaaa ttccctgaag ttgatgtgtt aacaaaatat
    11401 tctcaaccag aagactcctt gattcccttt tttgagataa ccgtgcctga atctcagtta
    11461 actgtgtccc agttcacgct tccaaaaagt gtttcagatg gcattgctgc tttggatcta
    11521 aatgcagtag ccaacaagat cgcagacttt gagttgccca ccatcatcgt gcctgagcag
    11581 accattgaga ttccctccat taagttctct gtacctgctg gaattgtcat tccttccttt
    11641 caagcactga ctgcacgctt tgaggtagac tctcccgtgt ataatgccac ttggagtgcc
    11701 agtttgaaaa acaaagcaga ttatgttgaa acagtcctgg attccacatg cagctcaacc
    11761 gtacagttcc tagaatatga actaaatgtt ttgggaacac acaaaatcga agatggtacg
    11821 ttagcctcta agactaaagg aacacttgca caccgtgact tcagtgcaga atatgaagaa
    11881 gatggcaaat atgaaggact tcaggaatgg gaaggaaaag cgcacctcaa tatcaaaagc
    11941 ccagcgttca ccgatctcca tctgcgctac cagaaagaca agaaaggcat ctccacctca
    12001 gcagcctccc cagccgtagg caccgtgggc atggatatgg atgaagatga cgacttttct
    12061 aaatggaact tctactacag ccctcagtcc tctccagata aaaaactcac catattcaaa
    12121 actgagttga gggtccggga atctgatgag gaaactcaga tcaaagttaa ttgggaagaa
    12181 gaggcagctt ctggcttgct aacctctctg aaagacaacg tgcccaaggc cacaggggtc
    12241 ctttatgatt atgtcaacaa gtaccactgg gaacacacag ggctcaccct gagagaagtg
    12301 tcttcaaagc tgagaagaaa tctgcagaac aatgctgagt gggtttatca aggggccatt
    12361 aggcaaattg atgatatcga cgtgaggttc cagaaagcag ccagtggcac cactgggacc
    12421 taccaagagt ggaaggacaa ggcccagaat ctgtaccagg aactgttgac tcaggaaggc
    12481 caagccagtt tccagggact caaggataac gtgtttgatg gcttggtacg agttactcaa
    12541 aaattccata tgaaagtcaa gaagctgatt gactcactca ttgattttct gaacttcccc
    12601 agattccagt ttccggggaa acctgggata tacactaggg aggaactttg cactatgttc
    12661 atgagggagg tagggacggt actgtcccag gtatattcga aagtccataa tggttcagaa
    12721 atactgtttt cctatttcca agacctagtg attacacttc ctttcgagtt aaggaaacat
    12781 aaactaatag atgtaatctc gatgtatagg gaactgttga aagatttatc aaaagaagcc
    12841 caagaggtat ttaaagccat tcagtctctc aagaccacag aggtgctacg taatcttcag
    12901 gaccttttac aattcatttt ccaactaata gaagataaca ttaaacagct gaaagagatg
    12961 aaatttactt atcttattaa ttatatccaa gatgagatca acacaatctt caatgattat
    13021 atcccatatg tttttaaatt gttgaaagaa aacctatgcc ttaatcttca taagttcaat
    13081 gaatttattc aaaacgagct tcaggaagct tctcaagagt tacagcagat ccatcaatac
    13141 attatggccc ttcgtgaaga atattttgat ccaagtatag ttggctggac agtgaaatat
    13201 tatgaacttg aagaaaagat agtcagtctg atcaagaacc tgttagttgc tcttaaggac
    13261 ttccattctg aatatattgt cagtgcctct aactttactt cccaactctc aagtcaagtt
    13321 gagcaatttc tgcacagaaa tattcaggaa tatcttagca tccttaccga tccagatgga
    13381 aaagggaaag agaagattgc agagctttct gccactgctc aggaaataat taaaagccag
    13441 gccattgcga cgaagaaaat aatttctgat taccaccagc agtttagata taaactgcaa
    13501 gatttttcag accaactctc tgattactat gaaaaattta ttgctgaatc caaaagattg
    13561 attgacctgt ccattcaaaa ctaccacaca tttctgatat acatcacgga gttactgaaa
    13621 aagctgcaat caaccacagt catgaacccc tacatgaagc ttgctccagg agaacttact
    13681 atcatcctct aattttttta aaagaaatct tcatttattc ttcttttcca attgaacttt
    13741 cacatagcac agaaaaaatt caaactgcct atattgataa aaccatacag tgagccagcc
    13801 ttgcagtagg cagtagacta taagcagaag cacatatgaa ctggacctgc accaaagctg
    13861 gcaccagggc tcggaaggtc tctgaactca gaaggatggc attttttgca agttaaagaa
    13921 aatcaggatc tgagttattt tgctaaactt gggggaggag gaacaaataa atggagtctt
    13981 tattgtgtat cat
//