LOCUS HUMSISPDG 3798 bp mRNA linear HUM 13-JAN-1995
DEFINITION Human c-sis/platelet-derived growth factor 2 (SIS/PDGF2) mRNA,
complete cds.
ACCESSION M12783 M16288
VERSION M12783.1
KEYWORDS growth factor; platelet-derived growth factor; proto-oncogene.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3798)
AUTHORS Rao,C.D., Igarashi,H., Pech,M.W., Robbins,K.C. and Aaronson,S.A.
TITLE Oncogenic potential of the human platelet-derived growth factor
transcriptional unit
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 51 (Pt 2), 959-966 (1986)
PUBMED 3472769
REFERENCE 2 (bases 1 to 3798)
AUTHORS Rao,C.D., Igarashi,H., Chiu,I.M., Robbins,K.C. and Aaronson,S.A.
TITLE Structure and sequence of the human c-sis/platelet-derived growth
factor 2 (SIS/PDGF2) transcriptional unit
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (8), 2392-2396 (1986)
PUBMED 3517869
COMMENT Original source text: Human placenta, cDNA to mRNA, clone pSD1;
DNA, clone pc-sis RH-9.
Computer-readable sequence kindly provided by C.D.Rao, 31-JUL-1986.
The first 70 and last 355 nucleotides in the sequence presented
below were derived from DNA. The normal human SIS/PDGF2 coding
sequence has the capacity to efficiently transform cells responsive
to its growth-promoting activity. The SIS/PDGF2 mRNA has been
detected in tumors such as glioblastomas and fibrosarcomas, whose
normal counterparts possess PDGF receptors and are responsive to
the growth-promoting action of the SIS/PDGF2 gene product.
FEATURES Location/Qualifiers
source 1..3798
/db_xref="H-InvDB:HIT000194179"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="22q12.3-q13.1"
/tissue_type="placenta"
gene 1..3798
/gene="PDGFB"
mRNA 71..3443
/gene="PDGFB"
/note="G00-120-709"
CDS 1093..1818
/gene="PDGFB"
/codon_start=1
/product="c-sis/platelet-derived growth factor 2"
/protein_id="AAA60553.1"
/db_xref="GDB:G00-120-709"
/translation="MNRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDL
QRLLHGDPGEEDGAELDLNMTRSHSGGELESLARGRRSLGSLTIAEPAMIAECKTRTE
VFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVR
KKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRP
PKGKHRKFKHTHDKTALKETLGA"
sig_peptide 1093..1137
/gene="PDGFB"
/note="G00-120-709"
mat_peptide 1138..1815
/gene="PDGFB"
/product="c-sis/platelet-derived growth factor 2"
/note="G00-120-709"
BASE COUNT 745 a 1216 c 1073 g 764 t
ORIGIN 666 bp upstream of DdeI site.
1 gaggaaaggc tgtctccacc cacctctcgc actctccctt ctcctttata aaggccggaa
61 cagctgaaag ggtggcaact tctcctcctg cggccgggag cggcctgcct gcctccctgc
121 gcacccgcag cctcccccgc tgcctcccta gggctcccct ccggccgcca gcgcccattt
181 ttcattccct agatagagat actttgcgcg cacacacata catacgcgcg caaaaaggaa
241 aaaaaaaaaa aaaagcccac cctccagcct cgctgcaaag agaaaaccgg agcagccgca
301 gctcgcagct cgcagctcgc agcccgcagc ccgcagagga cgcccagagc ggcgagcagg
361 cgggcagacg gaccgacgga ctcgcgccgc gtccacctgt cggccgggcc cagccgagcg
421 cgcagcgggc acgccgcgcg cgcggagcag ccgtgcccgc cgcccgggcc cgccgccagg
481 gcgcacacgc tcccgccccc ctacccggcc cgggcgggag tttgcacctc tccctgcccg
541 ggtgctcgag ctgccgttgc aaagccaact ttggaaaaag ttttttgggg gagacttggg
601 ccttgaggtg cccagctccg cgctttccga ttttgggggc ctttccagaa aatgttgcaa
661 aaaagctaag ccggcgggca gaggaaaacg cctgtagccg gcgagtgaag acgaaccatc
721 gactgccgtg ttccttttcc tcttggaggt tggagtcccc tgggcgcccc cacacggcta
781 gacgcctcgg ctggttcgcg acgcagcccc ccggccgtgg atgctgcact cgggctcggg
841 atccgcccag gtagccggcc tcggacccag gtcctgcgcc caggtcctcc cctgcccccc
901 agcgacggag ccggggccgg gggcggcggc gccgggggca tgcgggtgag ccgcggctgc
961 agaggcctga gcgcctgatc gccgcggacc tgagccgagc ccacccccct ccccagcccc
1021 ccaccctggc cgcgggggcg gcgcgctcga tctacgcgtc cggggccccg cggggccggg
1081 cccggagtcg gcatgaatcg ctgctgggcg ctcttcctgt ctctctgctg ctacctgcgt
1141 ctggtcagcg ccgaggggga ccccattccc gaggagcttt atgagatgct gagtgaccac
1201 tcgatccgct cctttgatga tctccaacgc ctgctgcacg gagaccccgg agaggaagat
1261 ggggccgagt tggacctgaa catgacccgc tcccactctg gaggcgagct ggagagcttg
1321 gctcgtggaa gaaggagcct gggttccctg accattgctg agccggccat gatcgccgag
1381 tgcaagacgc gcaccgaggt gttcgagatc tcccggcgcc tcatagaccg caccaacgcc
1441 aacttcctgg tgtggccgcc ctgtgtggag gtgcagcgct gctccggctg ctgcaacaac
1501 cgcaacgtgc agtgccgccc cacccaggtg cagctgcgac ctgtccaggt gagaaagatc
1561 gagattgtgc ggaagaagcc aatctttaag aaggccacgg tgacgctgga agaccacctg
1621 gcatgcaagt gtgagacagt ggcagctgca cggcctgtga cccgaagccc ggggggttcc
1681 caggagcagc gagccaaaac gccccaaact cgggtgacca ttcggacggt gcgagtccgc
1741 cggcccccca agggcaagca ccggaaattc aagcacacgc atgacaagac ggcactgaag
1801 gagacccttg gagcctaggg gcatcggcag gagagtgtgt gggcagggtt atttaatatg
1861 gtatttgctg tattgccccc atggggtcct tggagtgata atattgtttc cctcgtccgt
1921 ctgtctcgat gcctgattcg gacggccaat ggtgcttccc ccacccctcc acgtgtccgt
1981 ccacccttcc atcagcgggt ctcctcccag cggcctccgg tcttgcccag cagctcaaag
2041 aagaaaaaga aggactgaac tccatcgcca tcttcttccc ttaactccaa gaacttggga
2101 taagagtgtg agagagactg atggggtcgc tctttggggg aaacgggttc cttcccctgc
2161 acctggcctg ggccacacct gagcgctgtg gactgtcctg aggagccctg aggacctctc
2221 agcatagcct gcctgatccc tgaacccctg gccagctctg aggggaggca cctccaggca
2281 ggccaggctg cctcggactc catggctaag accacagacg ggcacacaga ctggagaaaa
2341 cccctcccac ggtgcccaaa caccagtcac ctcgtctccc tggtgcctct gtgcacagtg
2401 gcttcttttc gttttcgttt tgaagacgtg gactcctctt ggtgggtgtg gccagcacac
2461 caagtggctg ggtgccctct caggtgggtt agagatggag tttgctgttg aggtggtgta
2521 gatggtgacc tgggtatccc ctgcctcctg ccaccccttc ctccccatac tccactctga
2581 ttcacctctt cctctggttc ctttcatctc tctacctcca ccctgcattt tcctcttgtc
2641 ctggcccttc agtctgctcc accaaggggc tcttgaaccc cttattaagg ccccagatga
2701 ccccagtcac tcctctctag ggcagaagac tagaggccag ggcagcaagg gacctgctca
2761 tcatattcca acccagccac gactgccatg taaggttgtg cagggtgtgt actgcacaag
2821 gacattgtat gcagggagca ctgttcacat catagataaa gctgatttgt atatttatta
2881 tgacaatttc tggcagatgt aggtaaagag gaaaaggatc cttttcctaa ttcacacaaa
2941 gactccttgt ggactggctg tgcccctgat gcagcctgtg gctggagtgg ccaaatagga
3001 gggagactgt ggtaggggca gggaggcaac actgctgtcc acatgacctc catttcccaa
3061 agtcctctgc tccagcaact gcccttccag gtgggtgtgg gacacctggg agaaggtctc
3121 caagggaggg tgcagccctc ttgcccgcac ccctccctgc ttgcacactt ccccatcttt
3181 gatccttctg agctccacct ctggtggctc ctcctaggaa accagctcgt gggctgggaa
3241 tgggggagag aagggaaaag atccccaaga ccccctgggg tgggatctga gctcccacct
3301 cccttcccac ctactgcact ttcccccttc ccgccttcca aaacctgctt ccttcagttt
3361 gtaaagtcgg tgattatatt tttgggggct ttccttttat tttttaaatg taaaatttat
3421 ttatattccg tatttaaagt tgtaaaaaaa aataaccaca aaataaaacc aaatgaatcc
3481 gccggaggtc tgtctgttgg catcgtgcgt gacaattaac ctttctgcct tggcaggatg
3541 tgccgacagc ttgcggcgtg ttcctctcac tctgggagcc tcaggcgtga tctcacacac
3601 tggcgtgcac atacacacac acacacatac atgctcacac atgcgtgcac atacacgcag
3661 gcctgcaact tgggggaggc ctctgtctgg cgggaagaag agacacacag gctactctgt
3721 tggtcttggt cctggcacag ctcctgacac gtgaacttgt gcgtgtctct ggcagtgacg
3781 agagataaat ttctgcag
//