LOCUS HUMA2M 4577 bp mRNA linear HUM 30-OCT-1994
DEFINITION Human alpha-2-macroglobulin mRNA, complete cds.
ACCESSION M11313
VERSION M11313.1
KEYWORDS alpha-2-macroglobulin; protease inhibitor.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4577)
AUTHORS Kan,C.C., Solomon,E., Belt,K.T., Chain,A.C., Hiorns,L.R. and Fey,G.
TITLE Nucleotide sequence of cDNA encoding human alpha 2-macroglobulin
and assignment of the chromosomal locus
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (8), 2282-2286 (1985)
PUBMED 2581245
COMMENT Original source text: Human liver, cDNA to mRNA, clone
p-alpha-2-M1.
Draft entry and computer-readable sequence for [1] kindly provided
by M.R.Gehring, 22-JAN-1987.
FEATURES Location/Qualifiers
source 1..4577
/db_xref="H-InvDB:HIT000194109"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="12p13.3-p12.3"
gene 1..4577
/gene="A2M"
mRNA <1..4577
/gene="A2M"
/product="a2M mRNA"
CDS 44..4468
/gene="A2M"
/note="alpha-2-macroglobulin precursor"
/codon_start=1
/protein_id="AAA51551.1"
/db_xref="GDB:G00-119-639"
/translation="MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETT
EKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMF
LTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNE
LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHP
FTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDAS
DCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVE
LTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEAN
YYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYL
VFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRT
GTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLAN
KVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPE
KDLTGFPGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSK
IRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWI
WDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTM
PYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTV
SWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKET
TFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGC
GEQNMVLFAPNIYVLDYLNETQQLTPEVKSKAIGYLNTGYQRQLNYKHYDGSYSTFGE
RYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLN
NAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYT
KALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSA
EVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALS
KYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEG
CVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNM
AIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQD
VPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA"
sig_peptide 44..112
/gene="A2M"
/note="alpha-2-macroglobulin signal peptide"
mat_peptide 113..4465
/gene="A2M"
/product="alpha-2-macroglobulin"
BASE COUNT 1229 a 1166 c 1086 g 1096 t
ORIGIN Chromosome 12; 1120 bp upstream of TaqI site.
1 gctacaatcc atctggtctc ctccagctcc ttctttctgc aacatgggga agaacaaact
61 ccttcatcca agtctggttc ttctcctctt ggtcctcctg cccacagacg cctcagtctc
121 tggaaaaccg cagtatatgg ttctggtccc ctccctgctc cacactgaga ccactgagaa
181 gggctgtgtc cttctgagct acctgaatga gacagtgact gtaagtgctt ccttggagtc
241 tgtcagggga aacaggagcc tcttcactga cctggaggcg gagaatgacg tactccactg
301 tgtcgccttc gctgtcccaa agtcttcatc caatgaggag gtaatgttcc tcactgtcca
361 agtgaaagga ccaacccaag aatttaagaa gcggaccaca gtgatggtta agaacgagga
421 cagtctggtc tttgtccaga cagacaaatc aatctacaaa ccagggcaga cagtgaaatt
481 tcgtgttgtc tccatggatg aaaactttca ccccctgaat gagttgattc cactagtata
541 cattcaggat cccaaaggaa atcgcatcgc acaatggcag agtttccagt tagagggtgg
601 cctcaagcaa ttttcttttc ccctctcatc agagcccttc cagggctcct acaaggtggt
661 ggtacagaag aaatcaggtg gaaggacaga gcaccctttc accgtggagg aatttgttct
721 tcccaagttt gaagtacaag taacagtgcc aaagataatc accatcttgg aagaagagat
781 gaatgtatca gtgtgtggcc tatacacata tgggaagcct gtccctggac atgtgactgt
841 gagcatttgc agaaagtata gtgacgcttc cgactgccac ggtgaagatt cacaggcttt
901 ctgtgagaaa ttcagtggac agctaaacag ccatggctgc ttctatcagc aagtaaaaac
961 caaggtcttc cagctgaaga ggaaggagta tgaaatgaaa cttcacactg aggcccagat
1021 ccaagaagaa ggaacagtgg tggaattgac tggaaggcag tccagtgaaa tcacaagaac
1081 cataaccaaa ctctcatttg tgaaagtgga ctcacacttt cgacagggaa ttcccttctt
1141 tgggcaggtg cgcctagtag atgggaaagg cgtccctata ccaaataaag tcatattcat
1201 cagaggaaat gaagcaaact attactccaa tgctaccacg gatgagcatg gccttgtaca
1261 gttctctatc aacaccacca acgttatggg tacctctctt actgttaggg tcaattacaa
1321 ggatcgtagt ccctgttacg gctaccagtg ggtgtcagaa gaacacgaag aggcacatca
1381 cactgcttat cttgtgttct ccccaagcaa gagctttgtc caccttgagc ccatgtctca
1441 tgaactaccc tgtggccata ctcagacagt ccaggcacat tatattctga atggaggcac
1501 cctgctgggg ctgaagaagc tctcctttta ttatctgata atggcaaagg gaggcattgt
1561 ccgaactggg actcatggac tgcttgtgaa gcaggaagac atgaagggcc atttttccat
1621 ctcaatccct gtgaagtcag acattgctcc tgtcgctcgg ttgctcatct atgctgtttt
1681 acctaccggg gacgtgattg gggattctgc aaaatatgat gttgaaaatt gtctggccaa
1741 caaggtggat ttgagcttca gcccatcaca aagtctccca gcctcacacg cccacctgcg
1801 agtcacagcg gctcctcagt ccgtctgcgc cctccgtgct gtggaccaaa gcgtgctgct
1861 catgaagcct gatgctgagc tctcggcgtc ctcggtttac aacctgctac cagaaaagga
1921 cctcactggc ttccctgggc ctttgaatga ccaggacgat gaagactgca tcaatcgtca
1981 taatgtctat attaatggaa tcacatatac tccagtatca agtacaaatg aaaaggatat
2041 gtacagcttc ctagaggaca tgggcttaaa ggcattcacc aactcaaaga ttcgtaaacc
2101 caaaatgtgt ccacagcttc aacagtatga aatgcatgga cctgaaggtc tacgtgtagg
2161 tttttatgag tcagatgtaa tgggaagagg ccatgcacgc ctggtgcatg ttgaagagcc
2221 tcacacggag accgtacgaa agtacttccc tgagacatgg atctgggatt tggtggtggt
2281 aaactcagca ggggtggctg aggtaggagt aacagtccct gacaccatca ccgagtggaa
2341 ggcaggggcc ttctgcctgt ctgaagatgc tggacttggt atctcttcca ctgcctctct
2401 ccgagccttc cagcccttct ttgtggagct tacaatgcct tactctgtga ttcgtggaga
2461 ggccttcaca ctcaaggcca cggtcctaaa ctaccttccc aaatgcatcc gggtcagtgt
2521 gcagctggaa gcctctcccg ccttccttgc tgtcccagtg gagaaggaac aagcgcctca
2581 ctgcatctgt gcaaacgggc ggcaaactgt gtcctgggca gtaaccccaa agtcattagg
2641 aaatgtgaat ttcactgtga gcgcagaggc actagagtct caagagctgt gtgggactga
2701 ggtgccttca gttcctgaac acggaaggaa agacacagtc atcaagcctc tgttggttga
2761 acctgaagga ctagagaagg aaacaacatt caactcccta ctttgtccat caggtggtga
2821 ggtttctgaa gaattatccc tgaaactgcc accaaatgtg gtagaagaat ctgcccgagc
2881 ttctgtctca gttttgggag acatattagg ctctgccatg caaaacacac aaaatcttct
2941 ccagatgccc tatggctgtg gagagcagaa tatggtcctc tttgctccta acatctatgt
3001 actggattat ctaaatgaaa cacagcagct tactccagag gtcaagtcca aggccattgg
3061 ctatctcaac actggttacc agagacagtt gaactacaaa cactatgatg gctcctacag
3121 cacctttggg gagcgatatg gcaggaacca gggcaacacc tggctcacag cctttgttct
3181 gaagactttt gcccaagctc gagcctacat cttcatcgat gaagcacaca ttacccaagc
3241 cctcatatgg ctctcccaga ggcagaagga caatggctgt ttcaggagct ctgggtcact
3301 gctcaacaat gccataaagg gaggagtaga agatgaagtg accctctccg cctatatcac
3361 catcgccctt ctggagattc ctctcacagt cactcaccct gttgtccgca atgccctgtt
3421 ttgcctggag tcagcctgga agacagcaca agaaggggac catggcagcc atgtatatac
3481 caaagcactg ctggcctatg cttttgccct ggcaggtaac caggacaaga ggaaggaagt
3541 actcaagtca cttaatgagg aagctgtgaa gaaagacaac tctgtccatt gggagcgccc
3601 tcagaaaccc aaggcaccag tggggcattt ttacgaaccc caggctccct ctgctgaggt
3661 ggagatgaca tcctatgtgc tcctcgctta tctcacggcc cagccagccc caacctcgga
3721 ggacctgacc tctgcaacca acatcgtgaa gtggatcacg aagcagcaga atgcccaggg
3781 cggtttctcc tccacccagg acacagtggt ggctctccat gctctgtcca aatatggagc
3841 cgccacattt accaggactg ggaaggctgc acaggtgact atccagtctt cagggacatt
3901 ttccagcaaa ttccaagtgg acaacaacaa tcgcctgtta ctgcagcagg tctcattgcc
3961 agagctgcct ggggaataca gcatgaaagt gacaggagaa ggatgtgtct acctccagac
4021 ctccttgaaa tacaatattc tcccagaaaa ggaagagttc ccctttgctt taggagtgca
4081 gactctgcct caaacttgtg atgaacccaa agcccacacc agcttccaaa tctccctaag
4141 tgtcagttac acagggagcc gctctgcctc caacatggcg atcgttgatg tgaagatggt
4201 ctctggcttc attcccctga agccaacagt gaaaatgctt gaaagatcta accatgtgag
4261 ccggacagaa gtcagcagca accatgtctt gatttacctt gataaggtgt caaatcagac
4321 actgagcttg ttcttcacgg ttctgcaaga tgtcccagta agagatctca aaccagccat
4381 agtgaaagtc tatgattact acgagacgga tgagtttgca atcgctgagt acaatgctcc
4441 ttgcagcaaa gatcttggaa atgcttgaag accacaaggc tgaaaagtgc tttgctggag
4501 tcctgttctc tgagctccac agaagacacg tgtttttgta tctttaaaga cttgatgaat
4561 aaacactttt tctggtc
//