LOCUS HUMIFNAIP 980 bp mRNA linear HUM 08-NOV-1994
DEFINITION Human interferon-alpha type I' mRNA, complete cds.
ACCESSION M11026
VERSION M11026.1
KEYWORDS alpha-interferon; interferon.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 980)
AUTHORS Mizoguchi,J., Pitha,P.M. and Raj,N.B.
TITLE Efficient expression in Escherichia coli of two species of human
interferon-alpha and their hybrid molecules
JOURNAL DNA 4 (3), 221-232 (1985)
PUBMED 3891272
COMMENT Original source text: Human Namalva (lymphoblastoid) cell line,
cDNA to mRNA, clone 78.
Draft entry and clean copy sequence for [1] kindly provided by
N.B.K.Raj, 17-OCT-1985.
The relative specific antiviral activities of IFN-alpha-A and
IFN-alpha-I' differ significantly on cells of different species,
while they are comparable on human cells.
The only differences between the interferon-alpha type I and type
I' are at position 219, where a 'c' in type I is replaced with an
'a' in type I', changing a proline to a histidine, and at position
571, where a type I 't' is replaced by type I' 'c', in which case
the amino acid remains a serine.
A putative polyadenylation signal is located at positions 963-968.
FEATURES Location/Qualifiers
source 1..980
/db_xref="H-InvDB:HIT000194096"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="9p22"
gene 1..980
/gene="IFNA"
mRNA <1..980
/gene="IFNA"
/product="IFNaI' mRNA"
CDS 50..619
/gene="IFNA"
/note="interferon-alpha I' precursor"
/codon_start=1
/protein_id="AAA52725.1"
/db_xref="GDB:G00-119-328"
/translation="MALSFSLLMAVLVLSYKSICSLGCDLPQTHSLGNRRALILLAQM
GRISPFSCLKDRHDFGLPQEEFDGNQFQKTQAISVLHEMIQQTFNLFSTEDSSAAWEQ
SLLEKFSTELYQQLNNLEACVIQEVGMEETPLMNEDSILAVRKYFQRITLYLTEKKYS
PCAWEVVRAEIMRSLSFSTNLQKILRRKD"
sig_peptide 50..118
/gene="IFNA"
/note="interferon-alpha I' signal peptide"
mat_peptide 119..616
/gene="IFNA"
/product="interferon-alpha I'"
BASE COUNT 283 a 214 c 181 g 302 t
ORIGIN 93 bp upstream of AluI site; chromosome 9pter-p13.
1 gttcaaggtt acccatctca agtagcctag caacatttgc aacatcccaa tggccctgtc
61 cttttcttta ctgatggccg tgctggtgct cagctacaaa tccatctgtt ctctaggctg
121 tgatctgcct cagacccaca gcctgggtaa taggagggcc ttgatactcc tggcacaaat
181 gggaagaatc tctcctttct cctgcctgaa ggacagacat gactttggac ttccccagga
241 ggagtttgat ggcaaccagt tccagaagac tcaagccatc tctgtcctcc atgagatgat
301 ccagcagacc ttcaatctct tcagcacaga ggactcatct gctgcttggg aacagagcct
361 cctagaaaaa ttttccactg aactttacca gcaactgaat aacctggaag catgtgtgat
421 acaggaggtt gggatggaag agactcccct gatgaatgag gactccatcc tggctgtgag
481 gaaatacttc caaagaatca ctctttatct aacagagaag aaatacagcc cttgtgcctg
541 ggaggttgtc agagcagaaa tcatgagatc cctctctttt tcaacaaact tgcaaaaaat
601 attaaggagg aaggattgaa aactggttca acatggcaat gatcctgatt gactaataca
661 ttatctcaca ctttcatgag ttcctcaatt tcaaagactc acttctataa ccaccacgag
721 ttgaatcaaa attttcaaat gttttcagca gtgtaaagaa gcgtcgtgta tacctgtgca
781 ggcactagta ctttacagat gaccatgctg atgtctctgt tcatctattt atttaaatat
841 ttatttaatt atttttaaga tttaaattat ttttttatgt aatatcatgt gtacctttac
901 attgtggtga atgtaacaat atatgttctt catatttagc caatatatta atttcctttt
961 tcattaaatt tttactatac
//