LOCUS HUMVWFM 6360 bp mRNA linear HUM 07-AUG-1995
DEFINITION Human von Willebrand factor mRNA, 3' end.
ACCESSION M10321 M10312 M10320 M12782
VERSION M10321.1
KEYWORDS glycoprotein; von Willebrand factor.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 404; 1648 to 6360)
AUTHORS Sadler,J.E., Shelton-Inloes,B.B., Sorace,J.M., Harlan,J.M.,
Titani,K. and Davie,E.W.
TITLE Cloning and characterization of two cDNAs coding for human von
Willebrand factor
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (19), 6394-6398 (1985)
PUBMED 2864688
REFERENCE 2 (bases 123 to 2056)
AUTHORS Shelton-Inloes,B.B., Titani,K. and Sadler,J.E.
TITLE cDNA sequences for human von Willebrand factor reveal five types of
repeated domains and five possible protein sequence polymorphisms
JOURNAL Biochemistry 25 (11), 3164-3171 (1986)
PUBMED 3488076
COMMENT Original source text: Human umbilical vein endothelial cell, cDNA
to mRNA, clones lambda-HvWF[1,3] [1]; clones lambda-HvWF[2,4] [2].
Draft entry and sequence in computer readable form for [2] kindly
provided by J.E.Sadler, 11-AUG-1986. The authors note numerous
repeated protein regions and domains The DNA used for sequencing
was obtained from a number of individuals. The variations are
probably alleles. A number of combinations with the variations
listed below is possible.
FEATURES Location/Qualifiers
source 1..6360
/db_xref="H-InvDB:HIT000194077"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="12pter-p12"
gene 1..6360
/gene="F8VWF"
mRNA <1..6360
/gene="F8VWF"
/product="vWF mRNA"
CDS <1..6226
/gene="F8VWF"
/note="von Willebrand factor prepropeptide"
/codon_start=2
/protein_id="AAB59512.1"
/db_xref="GDB:G00-119-125"
/translation="WATCPGSLLPDAVLSSPLSHRSKRSLSCRPHMVKLVCPADNLRA
EGLECAKTCQNYDLECMSMGSVSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGET
VKIGCNTCVCRDRKWNCTDHVCDATCSTIGMAHYLTFDGLKYLFPGECQYVLVQDYCG
SNPGTFRILVGNKGCSHPSVKCKKRVTILVEGGEIELFDGEVNVKRPMKDETHFEVVE
SGRYIILLLGKALSVVWDRHLSISVVLKQTYQEKVCGLCGNFDGIQNNDLTSSNLQVE
EDPVDFGNSWKVSSQCADTRKVPLDSSPATCHNNIMKQTMVDSSCRILTSDVFQDCNK
LVDPEPYLDVCIYDTCSCESIGDCACFCDTIAAYAHVCAQHGKVVTWRTATLCPQSCE
ERNLRENGYECEWRYNSCAPACQVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQT
CVDPEDCPVCEVAGRRFASGKKVTLNPSDPEHCQICHCDVVNLTCEACQEPGGLVVPP
TDAPVSPTTLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMME
RLRISQKWVRVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKY
TLFQIFSKIDRPEASRIALLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHAN
LKQIRLIEKQAPENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVTVG
PGLLGVSTLGPKRNSMVLDVAFVLEGSDKIGEADFNRSKEFMEEVIQRMDVGQDSIHV
TVLQYSYMVTVEYPFSEAQSKGDILQRVREIRYQGGNRTNTGLALRYLSDHSFLVSQG
DREQAPNLVYMVTGNPASDEIKRLPGDIQVVPIGVGPNANVQELERIGWPNAPILIQD
FETLPREAPDLVLQRCCSGEGLQIPTLSPAPDCSQPLDVILLLDGSSSFPASYFDEMK
SFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQ
IGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGI
GDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSGFVRICMDEDGN
EKRPGDVWTLPDQCHTVTCQPDGQTLLKSHRVNCDRGLRPSCPNSQSPVKVEETCGCR
WTCPCVCTGSSTRHIVTFDGQNFKLTGSCSYVLFQNKEQDLEVILHNGACSPGARQGC
MKSIEVKHSALSVELHSDMEVTVNGRLVSVPYVGGNMEVNVYGAIMHEVRFNHLGHIF
TFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQ
RPGQTCQPILEEQCLVPDSSHCQVLLLPLFAECHKVLAPATFYAICQQDSSHQEQVCE
VIASYAHLCRTNGVCVDWRTPDFCAMSCPPSLVYNHCEHGCPRHCDGNVSSCGDHPSE
GCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQPCQICTCLSGRKVNC
TTQPCPTAKAPTCGLCEVARLRQNADQCCPEYECVCDPVSCDLPPVPHCERGLQPTLT
NPGECRPNFTCACRKEECKRVSPPSCPPHRLPTLRKTQCCDEYECACNCVNSTVSCPL
GYLASTATNDCGCTTTTCLPDKVCVHRSTIYPVGQFWEEGCDVCTCTDMEDAVMGLRV
AQCSQKPCEDSCRSGFTYVLHEGECCGRCLPSACEVVTGSPRGDSQSSWKSVGSQWAS
PENPCLINECVRVKEEVFIQQRNVSCPQLEVPVCPSGFQLSCKTSACCPSCRCERMEA
CMLNGTVIGPGKTVMIDVCTTCRCMVQVGVISGFKLECRKTTCNPCPLGYKEENNTGE
CCGRCLPTACTIQLRGGQIMTLKRDETLQDGCDTHFCKVNERGEYFWEKRVTGCPPFD
EHKCLAEGGKIMKIPGTCCDTCEEPECNDITARLQYVKVGSCKSEVEVDIHYCQGKCA
SKAMYSIDINDVQDQCSCCSPTRTEPMQVALHCTNGSVVYHEVLNAMECKCSPRKCSK
"
mat_peptide 74..6223
/gene="F8VWF"
/product="von Willebrand factor mature peptide"
variation 149
/gene="F8VWF"
/note="g in lambda-HvWF2; a in lambda-HvWF[1,4]"
/replace="a"
variation 169
/gene="F8VWF"
/note="t in lambda-HvWF[1,4]; c in lambda-HvWF2"
/replace="c"
variation 339
/gene="F8VWF"
/note="g in lambda-HvWF[1,2]; a in lambda-HvWF4"
/replace="a"
variation 353
/gene="F8VWF"
/note="a in lambda-HvWF[1,2]; g in lambda-HvWF4"
/replace="g"
variation 664
/gene="F8VWF"
/note="g in lambda-HvWF4; a in lambda-HvWF2"
/replace="a"
variation 1648
/gene="F8VWF"
/note="g in lambda-HvWF4; t in lambda-HvWF3"
/replace="t"
variation 1795
/gene="F8VWF"
/note="g in lambda-HvWF4; a in lambda-HvWF3"
/replace="a"
variation 1925
/gene="F8VWF"
/note="g in lambda-HvWF3; a in lambda-HvWF4"
/replace="a"
BASE COUNT 1336 a 1812 c 1877 g 1335 t
ORIGIN Chromosome 12pter-p12; 19 bp upsteam of HindIII site.
1 ctgggccacg tgccccggaa gcttgctgcc tgacgctgtc ctcagcagtc ccctgtctca
61 tcgcagcaaa aggagcctat cctgtcggcc ccacatggtc aagctggtgt gtcccgctga
121 caacctgcgg gctgaagggc tcgagtgtgc caaaacgtgc cagaactatg acctggagtg
181 catgagcatg ggctctgtct ctggctgcct ctgccccccg ggcatggtcc ggcatgagaa
241 cagatgtgtg gccctggaaa ggtgtccctg cttccatcag ggcaaggagt atgcccctgg
301 agaaacagtg aagattggct gcaacacttg tgtctgtcgg gaccggaagt ggaactgcac
361 agaccatgtg tgtgatgcca cgtgctccac gatcggcatg gcccactacc tcaccttcga
421 cgggctcaaa tacctgttcc ccggggagtg ccagtacgtt ctggtgcagg attactgcgg
481 cagtaaccct gggacctttc ggatcctagt ggggaataag ggatgcagcc acccctcagt
541 gaaatgcaag aaacgggtca ccatcctggt ggagggagga gagattgagc tgtttgacgg
601 ggaggtgaat gtgaagaggc ccatgaagga tgagactcac tttgaggtgg tggagtctgg
661 ccggtacatc attctgctgc tgggcaaagc cctctccgtg gtctgggacc gccacctgag
721 catctccgtg gtcctgaagc agacatacca ggagaaagtg tgtggcctgt gtgggaattt
781 tgatggcatc cagaacaatg acctcaccag cagcaacctc caagtggagg aagaccctgt
841 ggactttggg aactcctgga aagtgagctc gcagtgtgct gacaccagaa aagtgcctct
901 ggactcatcc cctgccacct gccataacaa catcatgaag cagacgatgg tggattcctc
961 ctgtagaatc cttaccagtg acgtcttcca ggactgcaac aagctggtgg accccgagcc
1021 atatctggat gtctgcattt acgacacctg ctcctgtgag tccattgggg actgcgcctg
1081 cttctgcgac accattgctg cctatgccca cgtgtgtgcc cagcatggca aggtggtgac
1141 ctggaggacg gccacattgt gcccccagag ctgcgaggag aggaatctcc gggagaacgg
1201 gtatgagtgt gagtggcgct ataatagctg tgcacctgcc tgtcaagtca cgtgtcagca
1261 ccctgagcca ctggcctgcc ctgtgcagtg tgtggagggc tgccatgccc actgccctcc
1321 agggaaaatc ctggatgagc ttttgcagac ctgcgttgac cctgaagact gtccagtgtg
1381 tgaggtggct ggccggcgtt ttgcctcagg aaagaaagtc accttgaatc ccagtgaccc
1441 tgagcactgc cagatttgcc actgtgatgt tgtcaacctc acctgtgaag cctgccagga
1501 gccgggaggc ctggtggtgc ctcccacaga tgccccggtg agccccacca ctctgtatgt
1561 ggaggacatc tcggaaccgc cgttgcacga tttctactgc agcaggctac tggacctggt
1621 cttcctgctg gatggctcct ccaggctgtc cgaggctgag tttgaagtgc tgaaggcctt
1681 tgtggtggac atgatggagc ggctgcgcat ctcccagaag tgggtccgcg tggccgtggt
1741 ggagtaccac gacggctccc acgcctacat cgggctcaag gaccggaagc gaccgtcaga
1801 gctgcggcgc attgccagcc aggtgaagta tgcgggcagc caggtggcct ccaccagcga
1861 ggtcttgaaa tacacactgt tccaaatctt cagcaagatc gaccgccctg aagcctcccg
1921 catcgccctg ctcctgatgg ccagccagga gccccaacgg atgtcccgga actttgtccg
1981 ctacgtccag ggcctgaaga agaagaaggt cattgtgatc ccggtgggca ttgggcccca
2041 tgccaacctc aagcagatcc gcctcatcga gaagcaggcc cctgagaaca aggccttcgt
2101 gctgagcagt gtggatgagc tggagcagca aagggacgag atcgttagct acctctgtga
2161 ccttgcccct gaagcccctc ctcctactct gccccccgac atggcacaag tcactgtggg
2221 cccggggctc ttgggggttt cgaccctggg gcccaagagg aactccatgg ttctggatgt
2281 ggcgttcgtc ctggaaggat cggacaaaat tggtgaagcc gacttcaaca ggagcaagga
2341 gttcatggag gaggtgattc agcggatgga tgtgggccag gacagcatcc acgtcacggt
2401 gctgcagtac tcctacatgg tgaccgtgga gtaccccttc agcgaggcac agtccaaagg
2461 ggacatcctg cagcgggtgc gagagatccg ctaccagggc ggcaacagga ccaacactgg
2521 gctggccctg cggtacctct ctgaccacag cttcttggtc agccagggtg accgggagca
2581 ggcgcccaac ctggtctaca tggtcaccgg aaatcctgcc tctgatgaga tcaagaggct
2641 gcctggagac atccaggtgg tgcccattgg agtgggccct aatgccaacg tgcaggagct
2701 ggagaggatt ggctggccca atgcccctat cctcatccag gactttgaga cgctcccccg
2761 agaggctcct gacctggtgc tgcagaggtg ctgctccgga gaggggctgc agatccccac
2821 cctctcccca gcacctgact gcagccagcc cctggacgtg atccttctcc tggatggctc
2881 ctccagtttc ccagcttctt attttgatga aatgaagagt ttcgccaagg ctttcatttc
2941 aaaagccaat atagggcctc gtctcactca ggtgtcagtg ctgcagtatg gaagcatcac
3001 caccattgac gtgccatgga acgtggtccc ggagaaagcc catttgctga gccttgtgga
3061 cgtcatgcag cgggagggag gccccagcca aatcggggat gccttgggct ttgctgtgcg
3121 atacttgact tcagaaatgc atggtgccag gccgggagcc tcaaaggcgg tggtcatcct
3181 ggtcacggac gtctctgtgg attcagtgga tgcagcagct gatgccgcca ggtccaacag
3241 agtgacagtg ttccctattg gaattggaga tcgctacgat gcagcccagc tacggatctt
3301 ggcaggccca gcaggcgact ccaacgtggt gaagctccag cgaatcgaag acctccctac
3361 catggtcacc ttgggcaatt ccttcctcca caaactgtgc tctggatttg ttaggatttg
3421 catggatgag gatgggaatg agaagaggcc cggggacgtc tggaccttgc cagaccagtg
3481 ccacaccgtg acttgccagc cagatggcca gaccttgctg aagagtcatc gggtcaactg
3541 tgaccggggg ctgaggcctt cgtgccctaa cagccagtcc cctgttaaag tggaagagac
3601 ctgtggctgc cgctggacct gcccctgcgt gtgcacaggc agctccactc ggcacatcgt
3661 gacctttgat gggcagaatt tcaagctgac tggcagctgt tcttatgtcc tatttcaaaa
3721 caaggagcag gacctggagg tgattctcca taatggtgcc tgcagccctg gagcaaggca
3781 gggctgcatg aaatccatcg aggtgaagca cagtgccctc tccgtcgagc tgcacagtga
3841 catggaggtg acggtgaatg ggagactggt ctctgttcct tacgtgggtg ggaacatgga
3901 agtcaacgtt tatggtgcca tcatgcatga ggtcagattc aatcaccttg gtcacatctt
3961 cacattcact ccacaaaaca atgagttcca actgcagctc agccccaaga cttttgcttc
4021 aaagacgtat ggtctgtgtg ggatctgtga tgagaacgga gccaatgact tcatgctgag
4081 ggatggcaca gtcaccacag actggaaaac acttgttcag gaatggactg tgcagcggcc
4141 aggacagacg tgccagccca tcctggagga gcagtgtctt gtccccgaca gctcccactg
4201 ccaggtcctc ctcttaccac tgtttgctga atgccacaag gtcctggctc cagccacatt
4261 ctatgccatc tgccagcagg acagttcgca ccaggagcaa gtgtgtgagg tgatcgcctc
4321 ttatgcccac ctctgtcgga ccaacggggt ctgcgttgac tggaggacac ctgatttctg
4381 tgctatgtca tgcccaccat ctctggtcta caaccactgt gagcatggct gtccccggca
4441 ctgtgatggc aacgtgagct cctgtgggga ccatccctcc gaaggctgtt tctgccctcc
4501 agataaagtc atgttggaag gcagctgtgt ccctgaagag gcctgcactc agtgcattgg
4561 tgaggatgga gtccagcacc agttcctgga agcctgggtc ccggaccacc agccctgtca
4621 gatctgcaca tgcctcagcg ggcggaaggt caactgcaca acgcagccct gccccacggc
4681 caaagctccc acgtgtggcc tgtgtgaagt agcccgcctc cgccagaatg cagaccagtg
4741 ctgccccgag tatgagtgtg tgtgtgaccc agtgagctgt gacctgcccc cagtgcctca
4801 ctgtgaacgt ggcctccagc ccacactgac caaccctggc gagtgcagac ccaacttcac
4861 ctgcgcctgc aggaaggagg agtgcaaaag agtgtcccca ccctcctgcc ccccgcaccg
4921 tttgcccacc cttcggaaga cccagtgctg tgatgagtat gagtgtgcct gcaactgtgt
4981 caactccaca gtgagctgtc cccttgggta cttggcctca accgccacca atgactgtgg
5041 ctgtaccaca accacctgcc ttcccgacaa ggtgtgtgtc caccgaagca ccatctaccc
5101 tgtgggccag ttctgggagg agggctgcga tgtgtgcacc tgcaccgaca tggaggatgc
5161 cgtgatgggc ctccgcgtgg cccagtgctc ccagaagccc tgtgaggaca gctgtcggtc
5221 gggcttcact tacgttctgc atgaaggcga gtgctgtgga aggtgcctgc catctgcctg
5281 tgaggtggtg actggctcac cgcgggggga ctcccagtct tcctggaaga gtgtcggctc
5341 ccagtgggcc tccccggaga acccctgcct catcaatgag tgtgtccgag tgaaggagga
5401 ggtctttata caacaaagga acgtctcctg cccccagctg gaggtccctg tctgcccctc
5461 gggctttcag ctgagctgta agacctcagc gtgctgccca agctgtcgct gtgagcgcat
5521 ggaggcctgc atgctcaatg gcactgtcat tgggcccggg aagactgtga tgatcgatgt
5581 gtgcacgacc tgccgctgca tggtgcaggt gggggtcatc tctggattca agctggagtg
5641 caggaagacc acctgcaacc cctgccccct gggttacaag gaagaaaata acacaggtga
5701 atgttgtggg agatgtttgc ctacggcttg caccattcag ctaagaggag gacagatcat
5761 gacactgaag cgtgatgaga cgctccagga tggctgtgat actcacttct gcaaggtcaa
5821 tgagagagga gagtacttct gggagaagag ggtcacaggc tgcccaccct ttgatgaaca
5881 caagtgtctg gctgagggag gtaaaattat gaaaattcca ggcacctgct gtgacacatg
5941 tgaggagcct gagtgcaacg acatcactgc caggctgcag tatgtcaagg tgggaagctg
6001 taagtctgaa gtagaggtgg atatccacta ctgccagggc aaatgtgcca gcaaagccat
6061 gtactccatt gacatcaacg atgtgcagga ccagtgctcc tgctgctctc cgacacggac
6121 ggagcccatg caggtggccc tgcactgcac caatggctct gttgtgtacc atgaggttct
6181 caatgccatg gagtgcaaat gctcccccag gaagtgcagc aagtgaggct gctgcagctg
6241 catgggtgcc tgctgctgcc tgccttggcc tgatggccag gccagagtgc tgccagtcct
6301 ctgcatgttc tgctcttgtg cccttctgag cccacaataa aggctgagct cttatcttgc
//