LOCUS HUMINSR 4723 bp mRNA linear HUM 06-JAN-1995
DEFINITION Human insulin receptor mRNA, complete cds.
ACCESSION M10051
VERSION M10051.1
KEYWORDS insulin receptor; tyrosine kinase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4723)
AUTHORS Ebina,Y., Ellis,L., Jarnagin,K., Edery,M., Graf,L., Clauser,E.,
Ou,J.-H., Masiarz,F., Kan,Y.W., Goldfine,I.D., Roth,R.A. and
Rutter,W.J.
TITLE The human insulin receptor cDNA: the structural basis for
hormone-activated transmembrane signalling
JOURNAL Cell 40 (4), 747-758 (1985)
PUBMED 2859121
COMMENT Original source text: Human placenta, cDNA to mRNA, clones
lambda-IR[1-15].
[1] suggests that the insulin receptor may be the cellular homolog
of the v-ros transforming (oncogene) protein. [1] notes
similarities between the insulin receptor and several growth factor
receptors and oncogenes. Insulin receptor is a heterodimer
consisting of 2 alpha and 2 beta subunits. Beta-prime may be a
cleavage product produced upon binding of insulin. [1] suggests
that translation may begin at the 'atg' start codon at positions
79-81 with protein cleavage occurring after position 120 to yield
the signal peptide. [1] gives illustrations of the various domains
present in the protein. A draft entry and sequence for [1] in
computer-readable form were kindly provided by K. Jarnagin
(30-JUL-1985).
FEATURES Location/Qualifiers
source 1..4723
/db_xref="H-InvDB:HIT000194074"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="19p13.3-p13.2"
gene 1..4723
/gene="INSR"
sig_peptide 137..219
/gene="INSR"
/note="insulin receptor signal peptide"
CDS 139..4287
/gene="INSR"
/note="insulin receptor precursor"
/codon_start=1
/protein_id="AAA59174.1"
/db_xref="GDB:G00-119-352"
/translation="MGTGGRRGAAAAPLLVAVAALLLGAAGHLYPGEVCPGMDIRNNL
TRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDL
FPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDW
SRILDSVEDNHIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKV
CPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQ
DWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGP
CPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEIS
GYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTTTQ
GKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDKASCENELLKFSYIRTS
FDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLR
SNDPKSQNHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSV
PLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSELFELDYCLKGLKLPS
RTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVV
FVPRKTSSGTGAEDPRPSRKRRSLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPF
EKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGP
VTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLR
GLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVI
GSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELG
QGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVR
LLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADG
MAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMA
PESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCP
ERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEME
FEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPR
SNPS"
mat_peptide 220..2424
/gene="INSR"
/product="insulin receptor alpha subunit"
mat_peptide 2425..4284
/gene="INSR"
/product="insulin receptor beta subunit"
mat_peptide 2425..2469
/gene="INSR"
/product="insulin receptor beta-prime subunit"
BASE COUNT 1068 a 1298 c 1311 g 1046 t
ORIGIN Chromosome 19p13.3-p13.2; 89 bp upstream of SacII site.
1 ggggggctgc gcggccgggt cggtgcgcac acgagaagga cgcgcggccc ccagcgctct
61 tgggggccgc ctcggagcat gacccccgcg ggccagcgcc gcgcgcctga tccgaggaga
121 ccccgcgctc ccgcagccat gggcaccggg ggccggcggg gggcggcggc cgcgccgctg
181 ctggtggcgg tggccgcgct gctactgggc gccgcgggcc acctgtaccc cggagaggtg
241 tgtcccggca tggatatccg gaacaacctc actaggttgc atgagctgga gaattgctct
301 gtcatcgaag gacacttgca gatactcttg atgttcaaaa cgaggcccga agatttccga
361 gacctcagtt tccccaaact catcatgatc actgattact tgctgctctt ccgggtctat
421 gggctcgaga gcctgaagga cctgttcccc aacctcacgg tcatccgggg atcacgactg
481 ttctttaact acgcgctggt catcttcgag atggttcacc tcaaggaact cggcctctac
541 aacctgatga acatcacccg gggttctgtc cgcatcgaga agaacaatga gctctgttac
601 ttggccacta tcgactggtc ccgtatcctg gattccgtgg aggataatca catcgtgttg
661 aacaaagatg acaacgagga gtgtggagac atctgtccgg gtaccgcgaa gggcaagacc
721 aactgccccg ccaccgtcat caacgggcag tttgtcgaac gatgttggac tcatagtcac
781 tgccagaaag tttgcccgac catctgtaag tcacacggct gcaccgccga aggcctctgt
841 tgccacagcg agtgcctggg caactgttct cagcccgacg accccaccaa gtgcgtggcc
901 tgccgcaact tctacctgga cggcaggtgt gtggagacct gcccgccccc gtactaccac
961 ttccaggact ggcgctgtgt gaacttcagc ttctgccagg acctgcacca caaatgcaag
1021 aactcgcgga ggcagggctg ccaccaatac gtcattcaca acaacaagtg catccctgag
1081 tgtccctccg ggtacacgat gaattccagc aacttgctgt gcaccccatg cctgggtccc
1141 tgtcccaagg tgtgccacct cctagaaggc gagaagacca tcgactcggt gacgtctgcc
1201 caggagctcc gaggatgcac cgtcatcaac gggagtctga tcatcaacat tcgaggaggc
1261 aacaatctgg cagctgagct agaagccaac ctcggcctca ttgaagaaat ttcagggtat
1321 ctaaaaatcc gccgatccta cgctctggtg tcactttcct tcttccggaa gttacgtctg
1381 attcgaggag agaccttgga aattgggaac tactccttct atgccttgga caaccagaac
1441 ctaaggcagc tctgggactg gagcaaacac aacctcacca ccactcaggg gaaactcttc
1501 ttccactata accccaaact ctgcttgtca gaaatccaca agatggaaga agtttcagga
1561 accaaggggc gccaggagag aaacgacatt gccctgaaga ccaatgggga caaggcatcc
1621 tgtgaaaatg agttacttaa attttcttac attcggacat cttttgacaa gatcttgctg
1681 agatgggagc cgtactggcc ccccgacttc cgagacctct tggggttcat gctgttctac
1741 aaagaggccc cttatcagaa tgtgacggag ttcgatgggc aggatgcgtg tggttccaac
1801 agttggacgg tggtagacat tgacccaccc ctgaggtcca acgaccccaa atcacagaac
1861 cacccagggt ggctgatgcg gggtctcaag ccctggaccc agtatgccat ctttgtgaag
1921 accctggtca ccttttcgga tgaacgccgg acctatgggg ccaagagtga catcatttat
1981 gtccagacag atgccaccaa cccctctgtg cccctggatc caatctcagt gtctaactca
2041 tcatcccaga ttattctgaa gtggaaacca ccctccgacc ccaatggcaa catcacccac
2101 tacctggttt tctgggagag gcaggcggaa gacagtgagc tgttcgagct ggattattgc
2161 ctcaaagggc tgaagctgcc ctcgaggacc tggtctccac cattcgagtc tgaagattct
2221 cagaagcaca accagagtga gtatgaggat tcggccggcg aatgctgctc ctgtccaaag
2281 acagactctc agatcctgaa ggagctggag gagtcctcgt ttaggaagac gtttgaggat
2341 tacctgcaca acgtggtttt cgtccccaga aaaacctctt caggcactgg tgccgaggac
2401 cctaggccat ctcggaaacg caggtccctt ggcgatgttg ggaatgtgac ggtggccgtg
2461 cccacggtgg cagctttccc caacacttcc tcgaccagcg tgcccacgag tccggaggag
2521 cacaggcctt ttgagaaggt ggtgaacaag gagtcgctgg tcatctccgg cttgcgacac
2581 ttcacgggct atcgcatcga gctgcaggct tgcaaccagg acacccctga ggaacggtgc
2641 agtgtggcag cctacgtcag tgcgaggacc atgcctgaag ccaaggctga tgacattgtt
2701 ggccctgtga cgcatgaaat ctttgagaac aacgtcgtcc acttgatgtg gcaggagccg
2761 aaggagccca atggtctgat cgtgctgtat gaagtgagtt atcggcgata tggtgatgag
2821 gagctgcatc tctgcgtctc ccgcaagcac ttcgctctgg aacggggctg caggctgcgt
2881 gggctgtcac cggggaacta cagcgtgcga atccgggcca cctcccttgc gggcaacggc
2941 tcttggacgg aacccaccta tttctacgtg acagactatt tagacgtccc gtcaaatatt
3001 gcaaaaatta tcatcggccc cctcatcttt gtctttctct tcagtgttgt gattggaagt
3061 atttatctat tcctgagaaa gaggcagcca gatgggccgc tgggaccgct ttacgcttct
3121 tcaaaccctg agtatctcag tgccagtgat gtgtttccat gctctgtgta cgtgccggac
3181 gagtgggagg tgtctcgaga gaagatcacc ctccttcgag agctggggca gggctccttc
3241 ggcatggtgt atgagggcaa tgccagggac atcatcaagg gtgaggcaga gacccgcgtg
3301 gcggtgaaga cggtcaacga gtcagccagt ctccgagagc ggattgagtt cctcaatgag
3361 gcctcggtca tgaagggctt cacctgccat cacgtggtgc gcctcctggg agtggtgtcc
3421 aagggccagc ccacgctggt ggtgatggag ctgatggctc acggagacct gaagagctac
3481 ctccgttctc tgcggccaga ggctgagaat aatcctggcc gccctccccc tacccttcaa
3541 gagatgattc agatggcggc agagattgct gacgggatgg cctacctgaa cgccaagaag
3601 tttgtgcatc gggacctggc agcgagaaac tgcatggtcg cccatgattt tactgtcaaa
3661 attggagact ttggaatgac cagagacatc tatgaaacgg attactaccg gaaagggggc
3721 aagggtctgc tccctgtacg gtggatggca ccggagtccc tgaaggatgg ggtcttcacc
3781 acttcttctg acatgtggtc ctttggcgtg gtcctttggg aaatcaccag cttggcagaa
3841 cagccttacc aaggcctgtc taatgaacag gtgttgaaat ttgtcatgga tggagggtat
3901 ctggatcaac ccgacaactg tccagagaga gtcactgacc tcatgcgcat gtgctggcaa
3961 ttcaacccca agatgaggcc aaccttcctg gagattgtca acctgctcaa ggacgacctg
4021 caccccagct ttccagaggt gtcgttcttc cacagcgagg agaacaaggc tcccgagagt
4081 gaggagctgg agatggagtt tgaggacatg gagaatgtgc ccctggaccg ttcctcgcac
4141 tgtcagaggg aggaggcggg gggccgggat ggagggtcct cgctgggttt caagcggagc
4201 tacgaggaac acatccctta cacacacatg aacggaggca agaaaaacgg gcggattctg
4261 accttgcctc ggtccaatcc ttcctaacag tgcctaccgt ggcgggggcg ggcaggggtt
4321 cccattttcg ctttcctctg gtttgaaagc ctctggaaaa ctcaggattc tcacgactct
4381 accatgtcca gtggagttca gagatcgttc ctatacattt ctgttcatct taaggtggac
4441 tcgtttggtt accaatttaa ctagtcctgc agaggattta actgtgaacc tggagggcaa
4501 ggggtttcca cagttgctgc tcctttgggg caacgacggt ttcaaaccag gattttgtgt
4561 tttttcgttc cccccacccg cccccagcag atggaaagaa agcacctgtt tttacaaatt
4621 cttttttttt tttttttttt tttttttttg ctggtgtctg agcttcagta taaaagacaa
4681 aacttcctgt ttgtggaaca aaatttcgaa agaaaaaacc aaa
//