LOCUS LT744250 4251 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM50, complete
sequence.
ACCESSION LT744250
VERSION LT744250.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4251)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..4251
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM50"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81125-G06."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1950
/transl_table=11
/note="hTRIM50"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX46962.1"
/translation="MAWQVSLPELEDRLQCPICLEVFKEPLMLQCGHSYCKGCLVSLS
CHLDAELRCPVCRQAVDGSSSLPNVSLARVIEALRLPGDPEPKVCVHHRNPLSLFCEK
DQELICGLCGLLGSHQHHPVTPVSTVYSRMKEELAALISELKQEQKKVDELIAKLVNN
RTRIVNESDVFSWVIRREFQELHHLVDEEKARCLEGIGGHTRGLVASLDMQLEQAQGT
RERLAQAECVLEQFGNEDHHKFIRKFHSMASRAEMPQARPLEGAFSPISFKPGLHQAD
IKLTVWKRLFRKVLPAPEPLKLDPATAHPLLELSKGNTVVQCGLLAQRRASQPERFDY
STCVLASRGFSCGRHYWEVVVGSKSDWRLGVIKGTASRKGKLNRSPEHGVWLIGLKEG
RVYEAFACPRVPLPVAGHPHRIGLYLHYEQGELTFFDADRPDDLRPLYTFQADFQGKL
YPILDTCWHERGSNSLPMVLPPPSGPGPLSPEQPTKL"
regulatory complement(1951..2048)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(2069..2085)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 2318..3328
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX46963.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 3791..4193
/note="pMB1 ori"
BASE COUNT 943 a 1225 c 1183 g 900 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gcttggcagg tgagcctgcc agagctggag gaccggcttc agtgtcccat
541 ctgcctggag gtcttcaagg agcccctgat gctgcagtgt ggccactctt actgcaaggg
601 ctgcctggtt tccctgtcct gccacctgga tgccgagctg cgctgccccg tgtgccggca
661 ggcggtggac ggcagcagct ccctgcccaa cgtctccctg gccagggtga tcgaagccct
721 gaggctccct ggggacccgg agcccaaggt ctgcgtgcac caccggaacc cgctcagcct
781 tttctgcgag aaggaccagg agctcatctg tggcctctgc ggtctgctgg gctcccacca
841 acaccacccg gtcacgcccg tctccaccgt ctacagccgc atgaaggagg agctcgcagc
901 cctcatctct gagctgaagc aggagcagaa aaaggtggat gagctcatcg ccaaactggt
961 gaacaaccgg acccgaatcg tcaatgagtc ggatgtcttc agctgggtga tccgccgcga
1021 gttccaggag ctgcaccacc tggtggatga ggagaaggcc cgctgcctgg aggggatagg
1081 gggtcacacc cgtggcctgg tggcctccct ggacatgcag ctggagcagg cccagggaac
1141 ccgggagcgg ctggcccaag ccgagtgtgt gctggaacag ttcggcaatg aggaccacca
1201 caagttcatc cggaagttcc actccatggc ctccagagca gagatgccgc aggcccggcc
1261 cttagaaggc gcattcagcc ccatctcctt caagccaggc ctccaccagg ctgacatcaa
1321 gctgaccgtg tggaaaaggc tcttccggaa agttttgcca gccccggagc ctctcaagtt
1381 ggaccctgcc actgcccacc cactcctgga gctctccaag ggcaacacgg tggtgcagtg
1441 cgggcttctg gcccagcggc gagccagcca gcctgagcgc ttcgactaca gcacctgcgt
1501 cctggccagc cgcggcttct cctgcggccg ccactactgg gaggtggtgg tgggcagcaa
1561 gagcgactgg cgcctggggg tcatcaaggg cacagccagc cgtaagggca agctgaacag
1621 gtcccccgag cacggcgtgt ggctgatcgg cctgaaggag ggccgggtgt acgaagcctt
1681 tgcctgcccc cgggtacccc tgcccgtggc cggccacccc caccgcatcg ggctctacct
1741 gcactatgag cagggcgaac tcaccttctt cgatgccgac cgccccgatg acctgcggcc
1801 gctctacacc ttccaggccg acttccaggg caagctctac cccatcctgg acacctgctg
1861 gcacgagagg ggcagcaact cgctgcccat ggtgctgccc ccgcccagcg ggcctggccc
1921 cctcagcccc gagcagccca ccaagctgta cccaactttc ttgtacaaag ttggcattat
1981 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
2041 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
2101 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
2161 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctgcc
2221 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
2281 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
2341 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
2401 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
2461 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
2521 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
2581 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
2641 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
2701 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
2761 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
2821 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
2881 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
2941 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
3001 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
3061 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
3121 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
3181 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
3241 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
3301 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
3361 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
3421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
3481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
3541 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
3601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
3661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
3721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
3781 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
3841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
3901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
3961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
4021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
4081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
4141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
4201 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc a
//