LOCUS LT739597 3975 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM10, complete
sequence.
ACCESSION LT739597
VERSION LT739597.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3975)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..3975
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM10"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81075-B12."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1674
/transl_table=11
/note="hTRIM10"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX37656.1"
/translation="MASAASVTSLADEVNCPICQGTLREPVTIDCGHNFCRACLTRYC
EIPGPDLEESPTCPLCKEPFRPGSFRPNWQLANVVENIERLQLVSTLGLGEEDVCQEH
GEKIYFFCEDDEMQLCVVCREAGEHATHTMRFLEDAAAPYREQIHKCLKCLRKEREEI
QEIQSRENKRMQVLLTQVSTKRQQVISEFAHLRKFLEEQQSILLAQLESQDGDILRQR
DEFDLLVAGEICRFSALIEELEEKNERPARELLTDIRSTLIRCETRKCRKPVAVSPEL
GQRIRDFPQQALPLQREMKMFLEKLCFELDYEPAHISLDPQTSHPKLLLSEDHQRAQF
SYKWQNSPDNPQRFDRATCVLAHTGITGGRHTWVWMARVPGDSGCCQFCSPPSVLGTE
VAA"
regulatory complement(1675..1772)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(1793..1809)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 2042..3052
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX37657.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 3515..3917
/note="pMB1 ori"
BASE COUNT 970 a 1030 c 1073 g 902 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gcctctgctg cctctgtgac cagcctggca gatgaagtca actgccccat
541 ctgtcagggt accctgaggg agccggtcac tatcgactgc ggccacaact tctgccgggc
601 ctgccttacc cgctactgtg agataccagg cccagacctg gaggagtccc ctacttgccc
661 actctgcaaa gaacccttcc gtcctgggag cttccggccc aactggcagc tggctaacgt
721 ggtggagaac attgagcgcc tccagctggt gtccacactg ggtttgggag aggaggatgt
781 ctgccaagag cacggagaga agatctactt cttctgtgag gatgatgaga tgcagttgtg
841 cgtggtgtgc cgggaggctg gggagcacgc tacccacacc atgcgcttcc tggaggatgc
901 agcggctccc tatagggaac aaatccataa gtgtcttaaa tgtctaagaa aagagagaga
961 ggagattcaa gaaatccagt caagagaaaa taaaaggatg caagtcctcc tgactcaggt
1021 gtccaccaag agacaacagg tgatttctga gttcgcacac ctgaggaagt ttctagagga
1081 acagcagagc atcctcttag cacaattgga gagccaggat ggggacatct tgaggcaacg
1141 ggatgaattt gatttgctgg ttgctgggga gatctgccgg tttagtgctc ttattgaaga
1201 actggaggag aagaatgaga ggccagcaag ggagctcctg acggacatca gaagcactct
1261 aataagatgt gaaaccagaa agtgccggaa accggtggct gtgtcgccag agctgggcca
1321 gaggattcgg gactttcccc agcaggccct cccgctgcag agggagatga agatgtttct
1381 ggaaaaacta tgctttgagt tggactatga gccagctcac atttctctag accctcagac
1441 ttcccacccc aagctcctct tgtccgagga ccaccagcga gctcagttct cctacaaatg
1501 gcagaactca ccagacaacc cccagcgttt tgaccgggcc acctgtgttc tggcccacac
1561 tggcatcaca ggggggagac acacgtgggt gtggatggcc agggtacctg gggactcagg
1621 ctgctgccag ttctgctcac caccatccgt gcttggcaca gaagtagctg catacccaac
1681 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
1741 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
1801 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
1861 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
1921 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
1981 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
2041 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
2101 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
2161 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
2221 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
2281 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
2341 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
2401 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
2461 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
2521 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
2581 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
2641 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
2701 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
2761 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
2821 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
2881 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
2941 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
3001 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
3061 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
3121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
3181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
3241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
3301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
3361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
3421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
3481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
3541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
3601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
3661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
3721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
3781 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
3841 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
3901 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
3961 ttaatgcagc tggca
//