LOCUS LT738840 4482 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM16, complete
sequence.
ACCESSION LT738840
VERSION LT738840.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4482)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..4482
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM16"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81067-A09."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>2181
/transl_table=11
/note="hTRIM16"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX36142.1"
/translation="MAELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEED
VGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCE
EHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGH
TIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQ
FGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAIS
NTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQE
FSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEEN
RKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGID
RKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSF
YGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVVTAP"
regulatory complement(2182..2279)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(2300..2316)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 2549..3559
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX36143.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 4022..4424
/note="pMB1 ori"
BASE COUNT 1103 a 1175 c 1211 g 993 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gctgagttgg atctaatggc tccagggcca ctgcccaggg ccactgctca
541 gcccccagcc cctctcagcc cagactctgg gtcacccagc ccagattctg ggtcagccag
601 cccagtggaa gaagaggacg tgggctcctc ggagaagctt ggcagggaga cggaggaaca
661 ggacagcgac tctgcagagc agggggatcc tgctggtgag gggaaagagg tcctgtgtga
721 cttctgcctt gatgacacca gaagagtgaa ggcagtgaag tcctgtctaa cctgcatggt
781 gaattactgt gaagagcact tgcagccgca tcaggtgaac atcaaactgc aaagccacct
841 gctgaccgag ccagtgaagg accacaactg gcgatactgc cctgcccacc acagcccact
901 gtctgccttc tgctgccctg atcagcagtg catctgccag gactgttgcc aggagcacag
961 tggccacacc atagtctccc tggatgcagc ccgcagggac aaggaggctg aactccagtg
1021 cacccagtta gacttggagc ggaaactcaa gttgaatgaa aatgccatct ccaggctcca
1081 ggctaaccaa aagtctgttc tggtgtcggt gtcagaggtc aaagcggtgg ctgaaatgca
1141 gtttggggaa ctccttgctg ctgtgaggaa ggcccaggcc aatgtgatgc tcttcttaga
1201 ggagaaggag caagctgcgc tgagccaggc caacggtatc aaggcccacc tggagtacag
1261 gagtgccgag atggagaaga gcaagcagga gctggagagg atggcggcca tcagcaacac
1321 tgtccagttc ttggaggagt actgcaagtt taagaacact gaagacatca ccttccctag
1381 tgtttacgta gggctgaagg ataaactctc gggcatccgc aaagttatca cggaatccac
1441 tgtacactta atccagttgc tggagaacta taagaaaaag ctccaggagt tttccaagga
1501 agaggagtat gacatcagaa ctcaagtgtc tgccgttgtt cagcgcaaat attggacttc
1561 caaacctgag cccagcacca gggaacagtt cctccaatat gcgtatgaca tcacgtttga
1621 cccggacaca gcacacaagt atctccggct gcaggaggag aaccgcaagg tcaccaacac
1681 cacgccctgg gagcatccct acccggacct ccccagcagg ttcctgcact ggcggcaggt
1741 gctgtcccag cagagtctgt acctgcacag gtactatttt gaggtggaga tcttcggggc
1801 aggcacctat gttggcctga cctgcaaagg catcgaccgg aaaggggagg agcgcaacag
1861 ttgcatttcc ggaaacaact tctcctggag cctccaatgg aacgggaagg agttcacggc
1921 ctggtacagt gacatggaga ccccactcaa agctggccct ttccggaggc tcggggtcta
1981 tatcgacttc ccgggaggga tcctttcctt ctatggcgta gagtatgata ccatgactct
2041 ggttcacaag tttgcctgca aattttcaga accagtctat gctgccttct ggctttccaa
2101 gaaggaaaac gccatccgga ttgtagatct gggagaggaa cccgagaagc cagcaccgtc
2161 cttggtggtg actgctccct acccaacttt cttgtacaaa gttggcatta taagaaagca
2221 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
2281 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
2341 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
2401 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
2461 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
2521 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
2581 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
2641 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
2701 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
2761 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
2821 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
2881 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
2941 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
3001 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
3061 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
3121 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
3181 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
3241 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
3301 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
3361 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
3421 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
3481 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
3541 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
3601 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
3661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
3721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
3781 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
3841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
3901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
3961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
4021 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
4081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
4141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
4201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
4261 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
4321 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
4381 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
4441 gcctctcccc gcgcgttggc cgattcatta atgcagctgg ca
//