LOCUS LT727522 4507 bp DNA circular SYN 06-FEB-2017 DEFINITION Bacterial expression vector pSIL2, complete sequence. ACCESSION LT727522 VERSION LT727522.1 KEYWORDS . SOURCE Bacterial expression vector pSIL2 ORGANISM Bacterial expression vector pSIL2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4507) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..4507 /organism="Bacterial expression vector pSIL2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2525." /db_xref="taxon:1944707" regulatory 24..105 /note="fd terminator" /regulatory_class="terminator" regulatory 134..215 /note="fd terminator" /regulatory_class="terminator" rep_origin 313..768 /note="f1 ori" rep_origin complement(772..1145) /note="pMB1 ori" misc_feature complement(1686..2546) /note="ampicillin sensitive" CDS complement(2861..3520) /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL88728.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" regulatory 3785..3821 /note="tac promoter" /regulatory_class="promoter" misc_feature 3822..3857 /note="5' UTR of lacZ, incl. RBS" misc_feature 3865..3881 /note="5' UTR of ompA, incl. RBS" CDS 3882..>3944 /transl_table=11 /note="ompA signal sequence" /protein_id="SJL88729.1" /translation="MKKTAIAIAVALAGFATVAQA" CDS <3948..4397 /codon_start=1 /transl_table=11 /note="mIL2 mature sequence" /protein_id="SJL88730.1" /translation="APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMEN YRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFIS NIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ" misc_feature 4398..4500 /note="3' UTR of mIL2" BASE COUNT 1198 a 1091 c 1018 g 1200 t ORIGIN 1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt 61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc 121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag 181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg 241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg 781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1981 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2221 ttttctgtga ctggtgacta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag 2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg 2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct 2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc 2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg 2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg 3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcg agctcgagct tactccccat 3781 ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa 3841 tttcacacag gaaacaggat cctagataac gagggcaaaa aatgaaaaag acagctatcg 3901 cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggcctcagca cccacttcaa 3961 gctccacttc aagctctaca gcggaagcac agcagcagca gcagcagcag cagcagcagc 4021 agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg atggagaatt 4081 acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc aagcaggcca 4141 cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg catgttctgg 4201 atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc agcaatatca 4261 gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa ttcgatgatg 4321 agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa agcatcatct 4381 caacaagccc tcaataacta tgtacctcct gcttacaaca cataaggctc tctatttatt 4441 taaatattta actttaattt atttttggat gtattgttta ctatcttttg taactactag 4501 aggtcga //