LOCUS LT727365 6928 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pEF-BOSbsscFvB1-2C11, complete sequence. ACCESSION LT727365 VERSION LT727365.1 KEYWORDS . SOURCE Mammalian expression vector pEF-BOSbsscFvB1-2C11 ORGANISM Mammalian expression vector pEF-BOSbsscFvB1-2C11 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6928) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6928 /organism="Mammalian expression vector pEF-BOSbsscFvB1-2C11" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3617." /db_xref="taxon:1945011" rep_origin 207..663 /note="M13-O" regulatory complement(918..1484) /note="polyA" /regulatory_class="terminator" CDS complement(1515..>2270) /codon_start=1 /transl_table=11 /note="sFv" /protein_id="SJL88243.1" /translation="EVKLVESGGGLVQPGKSLKLSCEASGFTFSGYGMHWVRQAPGRG LESVAYITSSSINIKYLDAVKGRFTVSRDNAKNLLFLQMNILKSEDTAMYYCARFDWD KNYWGQGTMVTVSSRSGGGGSGGGGSGGGGSMDIQMTQSPSSLPASLGDRVTINCQAS QDISNYLNWYQQKPGKAPKLLIYYTNKLADGVPSRFSGSGSGRDSSFTISSLESEDIG SYYCQQYYNYPWTFGPGTKLEIKGSRSHHHHHH" CDS complement(<2325..>3047) /codon_start=1 /transl_table=11 /note="B1sFv" /protein_id="SJL88244.1" /translation="EVKLVESGGGLVKPGGSLKLSCAASGFAFSTYDMSWVRQTPEKR LEWVATISRGGNYTYYPDSVKGRFTISRDNARNALYLQMGSLRSEDTALYYCASPNWG GGYFDVWGAGTTVTVSSGGGGSGGGGSGGGGSDIVMTQSQKFMSTSVGDRVSITCKAS QNVGTDVAWYQQKLGQSPKLLIDSTSSRYTGVPDRFTGSGSGTDFTLTISNVQSEDLA DYFCQQYSNYPLTFGAGTKLELK" misc_feature complement(3048..3104) /note="sB1H" regulatory complement(3193..4336) /note="hEF1" /regulatory_class="promoter" misc_feature complement(3202..4140) /note="intron" CDS complement(<4377..4446) /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL88245.1" /translation="MDKVLNREESLQLMDLLGLERSA" rep_origin complement(4485..4560) /note="SV40 ori" regulatory complement(4517..4688) /note="SV40 early promoter" /regulatory_class="promoter" regulatory complement(4750..4833) /note="lac" /regulatory_class="promoter" rep_origin complement(4925..5327) /note="pMB1 ori" CDS complement(5868..6728) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88246.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1658 a 1899 c 1758 g 1613 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt 661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt 901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga 961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc 1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc 1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc 1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga 1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa 1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc 1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc 1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac 1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgatcga 1501 cctgcagccc aagcttagtg atggtgatgg tgatgagatc tggatccttt tatttccagc 1561 ttggtgccag gtccgaacgt ccacggatag ttataatact gttgacagta ataagatcca 1621 atatcttcgg attccaggct gctgatagtg aaagaagaat ctctcccaga accactgcca 1681 ctgaaccttg atgggactcc atctgccaat ttatttgtat aatagatcag gagcttagga 1741 gctttccctg gtttctgctg gtaccagttt aaataattgc taatgtcctg actggcctga 1801 caattgatag tgactctgtc tcccagggag gcaggcagtg atgatggaga ctgggtcatc 1861 tggatgtcca tcgatccgcc accgccagag ccacctccgc ctgaaccgcc tccaccagat 1921 cttgaggaga cggtgaccat ggttccttgg ccccagtaat ttttgtccca gtcgaatctt 1981 gcacagtagt acatggctgt gtcctcagac ttgagaatgt tcatttgtag aaacagtaag 2041 ttcttggcat tgtctctgga gacggtgaac cggcctttca cagcgtccaa gtatttgata 2101 ttaatactac tactagtaat gtatgcgacc gactccagcc ccctccctgg agcctggcgg 2161 acccagtgca tgccatagcc gctgaaggtg aatccagagg cctcacagga gagtttcagg 2221 gactttccag gctgcaccaa gcctccccca gactccacca gcttgacctc catcgatccg 2281 ccaccgccag agccacctcc gcctgaaccg cctccaccag atcctttcag ctccagcttg 2341 gtcccagcac cgaacgtgag aggatagttg ctatattgtt ggcagaaata atctgccagg 2401 tcttcagact gcacattgct gatggtgaga gtgaaatctg tcccagatcc actgcctgtg 2461 aagcgatcag ggactccagt gtaccgactg gatgtcgagt caatcagtag tttaggagat 2521 tgtcctagtt tctgttgata ccaggctaca tcagtaccca cattctgact ggccttgcag 2581 gtgatgctga ccctgtctcc tactgatgtg gacatgaatt tttgagactg ggtcatcaca 2641 atgtccgatc cgccaccgcc agagccacct ccgcctgaac cgcctccacc tgaggagaca 2701 gtgaccgtgg tccctgcgcc ccagacatcg aagtaccccc ccccccagtt aggacttgca 2761 cagtaataca aggccgtgtc ctcagacctc agactgccca tttgcaggta cagggcgttc 2821 ctggcattgt ctctggagat ggtgaatcgg cccttcacac tgtctggata gtaggtgtaa 2881 ttaccaccac gactaatggt tgcgacccac tccagcctct tctccggagt ctggcgaacc 2941 caagacatgt cataggtact gaaagcgaat ccagaggctg cacaggagag tttcagggac 3001 cctccaggct tcactaagcc tcccccagac tccaccagct tcacttcaca ctggacacct 3061 tttaaaatta ggacaaggaa aatcaagctc agcccaaagt tcatggtgag gggtccgtgt 3121 tcagtgctga tcactgaatg tgaggaactg ggaattcgag ctcgcccggg agtcgatcct 3181 ctagagaatt cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg 3241 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa 3301 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata 3361 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg 3421 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc 3481 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt 3541 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga 3601 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag 3661 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc 3721 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa 3781 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa 3841 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag 3901 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc 3961 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact 4021 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg 4081 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac 4141 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat 4201 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca 4261 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt 4321 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgaggc 4381 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt 4441 atccatcttt gcaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4501 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4561 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4621 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4681 gggagcctaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 4741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 4801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 4861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 4921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 4981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 5041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 5101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 5161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 5221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 5281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 5341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 5401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 5461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 5521 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 5581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 5641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 5701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 5761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 5821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 5881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 5941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 6001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 6061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 6121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 6181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 6241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 6301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 6361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 6421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 6481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 6541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 6601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 6661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 6721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 6781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 6841 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 6901 aataggcgta tcacgaggcc ctttcgtc //