LOCUS LT727184 6750 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pEF1mBID, complete sequence. ACCESSION LT727184 VERSION LT727184.1 KEYWORDS . SOURCE Mammalian expression vector pEF1mBID ORGANISM Mammalian expression vector pEF1mBID other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6750) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6750 /organism="Mammalian expression vector pEF1mBID" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4325." /db_xref="taxon:1944982" regulatory 508..1651 /note="hEF1 promoter" /regulatory_class="promoter" misc_feature 704..1642 /note="intron hEF1 promoter" regulatory 1668..1684 /note="T7 promoter" /regulatory_class="promoter" CDS 1754..2341 /transl_table=11 /note="mBID" /protein_id="SJL87697.1" /translation="MDSEVSNGSGLGAKHITDLLVFGFLQSSGCTRQELEVLGRELPV QAYWEADLEDELQTDGSQASRSFNQGRIEPDSESQEEIIHNIARHLAQIGDEMDHNIQ PTLVRQLAAQFMNGSLSEEDKRNCLAKALDEVKTAFPRDMENDKAMLIMTMLLAKKVA SHAPSLLRDVFHTTVNFINQNLFSYVRNLVRNEMD" misc_feature 2381..2422 /note="V5 epitope" misc_feature 2432..2449 /note="His-tag" regulatory 2478..2702 /note="BGH polyA" /regulatory_class="terminator" rep_origin 2748..3176 /note="f1 ori" regulatory 3181..3462 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 3419..3494 /note="SV40 ori" CDS 3586..4380 /transl_table=11 /note="neomycin" /protein_id="SJL87698.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory complement(4556..4630) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 4556..4630 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(4769..4852) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4944..5335) /note="pMB1 ori" CDS complement(5876..6736) /transl_table=11 /note="ampicillin" /protein_id="SJL87699.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1492 a 1763 c 1832 g 1663 t ORIGIN 1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1741 tgtggtggaa ttcatggact ctgaggtcag caacggttcc ggcctggggg ccaagcacat 1801 cacagacctg ctggtgttcg gctttctcca aagctctggc tgtactcgcc aagagctgga 1861 ggtgctgggt cgggaactgc ctgtgcaagc ttactgggag gcagacctcg aagacgagct 1921 gcagacagac ggcagccagg ccagccgctc cttcaaccaa ggaagaatag agccagattc 1981 tgaaagtcag gaagaaatca tccacaacat tgccagacat ctcgcccaaa taggcgatga 2041 gatggaccac aacatccagc ccacactggt gagacagcta gccgcacagt tcatgaatgg 2101 cagcctgtcg gaggaagaca aaaggaactg cctggccaaa gcccttgatg aggtgaagac 2161 agccttcccc agagacatgg agaacgacaa ggccatgctg ataatgacaa tgctgttggc 2221 caaaaaagtg gccagtcacg caccatcttt gctccgtgat gtcttccaca cgactgtcaa 2281 ctttattaac cagaacctat tctcctatgt gaggaacttg gttagaaacg agatggactg 2341 atccagcaca gtggcggccg ctcgaggtca cccattcgaa ggtaagccta tccctaaccc 2401 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac 2461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 2521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 2581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 2641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 2701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 2761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 2821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 2881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 2941 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 3001 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 3061 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 3121 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 3181 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 3241 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 3301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 3361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 3421 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 3481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 3541 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 3601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 3661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 3721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 3781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 3841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 3901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 3961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4081 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 4141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4381 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 4441 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 4501 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 4561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 4621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 4681 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 4741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 4801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 4861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 4921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg 5101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6721 aatgttgaat actcatactc ttcctttttc //