LOCUS LT727151 5471 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-EhRac1-DN, complete sequence. ACCESSION LT727151 VERSION LT727151.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-EhRac1-DN ORGANISM Mammalian expression vector pCAGGS-EhRac1-DN other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5471) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5471 /organism="Mammalian expression vector pCAGGS-EhRac1-DN" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4659." /db_xref="taxon:1944780" CDS <4..639 /codon_start=1 /transl_table=11 /note="hRac1-DN" /protein_id="SJL87607.1" /translation="NSTMGAPVPYPDPLEPRAAAQAIKCVVVGDGAVGKNCLLISYTT NAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFS LVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQ GLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL" misc_feature 16..54 /note="E-tag" regulatory 761..852 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 853..1209 /note="3' FR of beta-globin" regulatory complement(1287..1369) /note="lac promoter" /regulatory_class="promoter" rep_origin 1511..1586 /note="SV40 ori" regulatory complement(1617..1691) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1617..1691 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(1806..2207) /note="pMB1 ori" CDS complement(2748..3608) /transl_table=11 /note="ampicillin" /protein_id="SJL87608.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3744..4121 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4127..5468 /note="AG promoter" /regulatory_class="promoter" misc_feature 4496..5420 /note="intron" BASE COUNT 1176 a 1436 c 1514 g 1345 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctcaggcca tcaagtgtgt ggtggtggga gacggagctg taggtaaaaa ttgcctactg 121 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat 181 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga 241 caagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt 301 tgcttttccc ttgtgagtcc tgcatcattt gaaaatgtcc gtgcaaagtg gtatcctgag 361 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg 421 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg 481 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc 541 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct 601 cccgtgaaga agaggaagag aaaatgcctg ctgttgtgac tcgagcccgg gacattaaac 661 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 721 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 781 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 841 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg 901 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 961 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 1021 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 1081 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 1141 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 1201 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg 1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 1321 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 1381 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata 1441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 1501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 1561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact 1621 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 1681 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 1741 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 1801 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 1861 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca 1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 2401 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2821 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2881 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2941 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3001 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3061 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 3181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 3301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 3361 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 3421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 3481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3601 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3661 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3721 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat 3781 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 3841 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 3901 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 3961 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 4021 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 4081 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 4141 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 4201 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg 4261 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca 4321 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 4381 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc 4441 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 4501 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 4561 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 4621 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 4681 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 4741 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 4801 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 4861 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 4921 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 4981 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc 5041 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt 5101 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga 5161 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg 5221 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc 5281 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg 5341 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg 5401 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 5461 ttggcaaaga a //