LOCUS LT727079 6467 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6mBclxLMycHis, complete sequence.
ACCESSION LT727079
VERSION LT727079.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6mBclxLMycHis
ORGANISM Mammalian expression vector pEF6mBclxLMycHis
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6467)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6467
/organism="Mammalian expression vector pEF6mBclxLMycHis"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5078."
/db_xref="taxon:1945008"
regulatory 386..1529
/note="hEF1a promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1a promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1635..>2333
/transl_table=11
/note="mBclxL"
/protein_id="SJL87370.1"
/translation="MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAE
RETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYR
RAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQ
VLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGKEGFNRWFLTGM
TVAGVVLLGSLFSRK"
misc_feature 2349..2378
/note="Myc epitope"
misc_feature 2394..2411
/note="His-tag"
regulatory 2440..2664
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2710..3138
/note="f1 ori"
regulatory 3143..3424
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3381..3456
/note="SV40 ori"
regulatory 3529..3594
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 3595..3993
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL87371.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 4151..4225
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4151..4225)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4364..4447
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4539..4930)
/note="pMB1 ori"
CDS complement(5471..6331)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87372.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1458 a 1634 c 1763 g 1612 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtaagcgg
1621 ccgcaatggc tagcatgtct cagagcaacc gggagctggt ggtcgacttt ctctcctaca
1681 agctttccca gaaaggatac agctggagtc agtttagtga tgtcgaagag aataggactg
1741 aggccccaga agaaactgaa gcagagaggg agacccccag tgccatcaat ggcaacccat
1801 cctggcacct ggcggatagc ccggccgtga atggagccac tggccacagc agcagtttgg
1861 atgcgcggga ggtgattccc atggcagcag tgaagcaagc gctgagagag gcaggcgatg
1921 agtttgaact gcggtaccgg agagcgttca gtgatctaac atcccagctt cacataaccc
1981 cagggaccgc gtatcagagc tttgagcagg tagtgaatga actctttcgg gatggagtaa
2041 actggggtcg catcgtggcc tttttctcct ttggcggggc actgtgcgtg gaaagcgtag
2101 acaaggagat gcaggtattg gtgagtcgga ttgcaagttg gatggccacc tatctgaatg
2161 accacctaga gccttggatc caggagaacg gcggctggga cacttttgtg gatctctacg
2221 ggaacaatgc agcagccgag agccggaaag gcaaggaggg cttcaaccgc tggtttctga
2281 cgggcatgac tgtggctggt gtggttctgc tgggctcact cttcagtcgg aagtctagag
2341 ggcccttcga acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc
2401 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
2461 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
2521 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
2581 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
2641 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
2701 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
2761 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
2821 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
2881 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
2941 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
3001 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
3061 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3121 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
3181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
3241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
3301 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
3361 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
3421 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
3481 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
3541 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
3601 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
3661 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
3721 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
3781 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
3841 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
3901 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
3961 ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg
4021 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
4081 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
4141 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
4261 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
4321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4381 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
4861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
4921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
5281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
5341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
5401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
5461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
5521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
5581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
5641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
5701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
5761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
5821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
5881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
5941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
6241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
6301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
6361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//