LOCUS LT726977 5631 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEGFP-hnRNPC1, complete sequence.
ACCESSION LT726977
VERSION LT726977.1
KEYWORDS .
SOURCE Mammalian expression vector pEGFP-hnRNPC1
ORGANISM Mammalian expression vector pEGFP-hnRNPC1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5631)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5631
/organism="Mammalian expression vector pEGFP-hnRNPC1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5219."
/db_xref="taxon:1945018"
regulatory 1..568
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 613..>1329
/transl_table=11
/note="EGFP"
/protein_id="SJL87100.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
CDS 1381..>2289
/transl_table=11
/note="hnRNPC1"
/protein_id="SJL87101.1"
/translation="MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIV
GCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRSAA
EMYGSVTEHPSPSPLLSSSFDLDYDFQRDYYDRMYSYPARVPPPPPIARAVVPSKRQR
VSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDLQAIKKELTQIKQKVDSLLENLEKME
KEQSKQAVEMKNDKSEEEQSSSSVKKDETNVKMESEGGADDSAEEGDLLDDDDNEDRG
DDQLELIKDDEKEAEEGEDDRDSANGG"
regulatory 2397..2493
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2397..2493)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2547..3002)
/note="f1 ori"
regulatory 3003..3098
/note="amp promoter"
/regulatory_class="promoter"
misc_feature 3099..3133
/note="5' UTR of amp, incl. RBS"
regulatory 3154..3435
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3392..3467
/note="SV40 ori"
CDS 3527..4321
/transl_table=11
/note="kanamycin/ neomycin"
/protein_id="SJL87102.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 4512..4581
/note="HSV-TK polyA"
/regulatory_class="terminator"
rep_origin 5299..5625
/note="pMB1 ori"
BASE COUNT 1416 a 1457 c 1499 g 1259 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
1381 atggccagca acgttaccaa caagacagat cctcgctcca tgaactcccg tgtattcatt
1441 gggaatctca acactcttgt ggtcaagaaa tctgatgtgg aggcaatctt ttcgaagtat
1501 ggcaaaattg tgggctgctc tgttcataag ggctttgcct tcgttcagta tgttaatgag
1561 agaaatgccc gggctgctgt agcaggagag gatggcagaa tgattgctgg ccaggtttta
1621 gatattaacc tggctgcaga gccaaaagtg aaccgaggaa aagcaggtgt gaaacgatct
1681 gcagcggaga tgtacgggtc agtaacagaa cacccttctc cgtcccctct actcagctcc
1741 tcttttgact tggactatga ctttcaacgg gactattatg ataggatgta cagttaccca
1801 gcacgtgtac ctcctcctcc tcctattgct cgggctgtag tgccctcgaa acgtcagcgt
1861 gtatcaggaa acacttcacg aaggggcaaa agtggcttca attctaagag tggacagcgg
1921 ggatcttcca agtctggaaa gttgaaagga gatgaccttc aggccattaa gaaggagctg
1981 acccagataa aacaaaaagt ggattctctc ctggaaaacc tggaaaaaat ggaaaaggaa
2041 cagagcaaac aagcagtaga gatgaagaat gataagtcag aagaggagca gagcagcagc
2101 tccgtgaaga aagatgagac taatgtgaag atggagtctg aggggggtgc agatgactct
2161 gctgaggagg gggacctact ggatgatgat gataatgaag atcgggggga tgaccagctg
2221 gagttgatca aggatgatga aaaagaggct gaggaaggag aggatgacag agacagcgcc
2281 aatggaggag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
2341 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
2401 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
2461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
2521 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
2581 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
2641 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
2701 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
2761 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
2821 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
2881 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
2941 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
3001 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
3061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
3121 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
3181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
3241 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
3301 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
3361 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
3421 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
3481 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
3541 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3601 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3661 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
3721 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3781 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3841 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3901 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
3961 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
4021 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
4081 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
4141 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
4201 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
4261 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
4321 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
4381 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
4441 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
4501 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
4561 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
4621 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
4681 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
4741 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
4801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
4861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
4921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
4981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
5041 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
5101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
5161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
5221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
5281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
5341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
5401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
5461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
5521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
5581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//