LOCUS LT009440 9809 bp DNA circular SYN 15-AUG-2016
DEFINITION Lentiviral vector pDG1i-tdTomato-V5-Blast, complete sequence.
ACCESSION LT009440
VERSION LT009440.1
KEYWORDS .
SOURCE Lentiviral vector pDG1i-tdTomato-V5-Blast
ORGANISM Lentiviral vector pDG1i-tdTomato-V5-Blast
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9809)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K.,
Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C.,
Vandenabeele P., Haustraete J., Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60(5), 252-259(2016).
PUBMED 27177818
FEATURES Location/Qualifiers
source 1..9809
/organism="Lentiviral vector pDG1i-tdTomato-V5-Blast"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent University,
Belgium) accession number LMBP 7942."
/db_xref="taxon:1783447"
regulatory complement(35..159)
/note="gateway recombination site attR1"
/regulatory_class="attenuator"
CDS 268..927
/transl_table=11
/gene="cat"
/product="chloramphenicol acetyl transferase"
/note="chloramphenicol resistance"
/protein_id="CVH74042.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 1269..1574
/transl_table=11
/gene="ccdB"
/product="ccdB"
/note="B gene of the control of cell death locus of the
Escherichia coli F plasmid"
/protein_id="CVH74044.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 1615..1739
/note="gateway recombination site attR2"
/regulatory_class="attenuator"
CDS 1771..3270
/transl_table=11
/gene="tdTomato"
/product="tdTomato"
/note="Orange-red fluorescent protein tandem dimer with V5
epitope tag"
/protein_id="CVH74045.1"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQT
AKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFED
GGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKG
EIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERS
EGRHHLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEG
EGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGF
KWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTER
LYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNED
YTIVEQYERSEGRHHLFLYGMDELYKEGPRFEGKPIPNPLLGLDSTRTG"
regulatory 3291..3573
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3530..3605
/note="SV40 ori"
regulatory 3678..3743
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 3744..4142
/transl_table=11
/gene="bsr"
/product="blasticidin S-resistance"
/protein_id="CVH74048.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 4212..4227
/note="HIV-1 cPPT"
regulatory 4228..4462
/note="RSV/HIV-1 dU3/3' LTR"
/regulatory_class="terminator"
regulatory 4534..4608
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4534..4608)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin 4744..4819
/note="SV40 ori"
regulatory complement(4853..4869)
/note="T7 promoter"
/regulatory_class="promoter"
rep_origin 5037..5492
/note="f1 origin"
CDS 5623..6483
/transl_table=11
/gene="bla"
/product="beta-lactamase"
/note="ampicillin resistance"
/protein_id="CVH74049.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 7024..7426
/note="pMB1 ori"
regulatory 7518..7601
/note="lac promoter"
/regulatory_class="promoter"
regulatory 7665..7681
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 7790..8118
/note="RSV/HIV-1 5' LTR"
/regulatory_class="promoter"
misc_feature 8229..8273
/note="HIV-1 psi packaging signal"
misc_feature 8783..9016
/note="HIV-1 Rev response element"
regulatory 9534..9801
/note="hCMVTRE promoter"
/regulatory_class="promoter"
BASE COUNT 2574 a 2372 c 2523 g 2340 t
ORIGIN
1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg
61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
1021 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
1561 tctggggaat ataaatgtca ggctccgtta tacacagcca gtctgcaggt cgaccatagt
1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga ggtcatcaaa
1801 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
1861 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
1921 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
1981 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
2041 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
2101 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
2161 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
2221 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
2281 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
2341 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
2401 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
2461 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
2521 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
2581 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
2641 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
2701 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
2761 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
2821 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
2881 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
2941 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
3001 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
3061 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
3121 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
3181 atggacgagc tgtacaagga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
3241 ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg
3301 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
3361 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
3421 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
3481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
3541 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
3601 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
3661 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
3721 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
3781 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
3841 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
3901 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
3961 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
4021 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
4081 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
4141 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
4201 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
4261 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
4321 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
4381 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
4441 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
4501 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
4561 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
4621 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
4681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
4741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
4801 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
4861 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
4921 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
4981 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
5041 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
5101 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
5161 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
5221 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
5281 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5341 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
5401 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5461 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
5521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
5581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
5641 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
5701 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
5761 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
5821 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5881 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5941 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
6001 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
6061 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
6121 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
6181 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
6241 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
6301 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
6361 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
6421 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
6481 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
6541 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
6601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
6661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
6721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
6781 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
6841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
7021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
7081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
7141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
7201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
7261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
7321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
7381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
7441 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
7501 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
7561 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
7621 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
7681 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
7741 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
7801 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
7861 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
7921 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
7981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
8041 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
8101 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
8161 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
8221 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
8281 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
8341 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
8401 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
8461 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
8521 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
8581 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
8641 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
8701 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
8761 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
8821 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
8881 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
8941 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
9001 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
9061 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
9121 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
9181 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
9241 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
9301 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
9361 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
9421 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
9481 tagtgaacgg atctcgacgg tatcgatggt ccgaggttct agacgagttt actccctatc
9541 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
9601 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
9661 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
9721 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
9781 gtttagtgaa ccgtcagatc gcaccggtc
//