LOCUS HUMLAMAA 5433 bp mRNA linear HUM 06-JAN-1995
DEFINITION Homo sapiens laminin-related protein (LamA3) mRNA, complete cds.
ACCESSION L34155
VERSION L34155.1
KEYWORDS basement membrane protein; epiligrin alpha 3 subunit; extracellular
matrix protein; laminin-related protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5433)
AUTHORS Ryan,M.C., Tizard,R., VanDevanter,D.R. and Carter,W.G.
TITLE Cloning of the LamA3 gene encoding the alpha 3 chain of the
adhesive ligand epiligrin. Expression in wound repair
JOURNAL J. Biol. Chem. 269 (36), 22779-22787 (1994)
PUBMED 8077230
COMMENT Original source text: Homo sapiens (tissue library: random-primed,
lambda gt11 (Clontech); dT-primed, plasmid [pcDNA II] (Oligo)) cDNA
to mRNA.
FEATURES Location/Qualifiers
source 1..5433
/db_xref="H-InvDB:HIT000193153"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="18q11.2"
/cell_line="keratinocytes"
/tissue_lib="random-primed, lambda gt11 (Clontech);
dT-primed, plasmid [pcDNA II] (Oligo)"
gene 1..5433
/gene="LamA3"
CDS 1..5142
/gene="LamA3"
/function="cell adhesion ligand for integrins; epithelial
cell expression"
/note="amino acid feature: G1 subdomain, aa 794 .. 970;
amino acid feature: G2 subdomain, aa 971 .. 1139; amino
acid feature: G3 subdomain, aa 1140 .. 1353; amino acid
feature: G4 subdomain, aa 1354 .. 1529; amino acid
feature: G5 subdomain, aa 1530 .. 1713"
/codon_start=1
/product="epiligrin alpha 3 subunit"
/protein_id="AAA59483.1"
/translation="MGWLWIFGAALGQCLGYSSQQQRVPFLQPPGQSQLQASYVEFRP
SQGCSPGYYRDHKGLYTGRCVPCNCNGHSNQCQDGSGICVNCQHNTAGEHCERCQEGY
YGNAVHGSCRACPCPHTNSFATGCVVNGGDVRCSCKAGYTGTQCERCAPGYFGNPQKF
GGSCQPCSCNSNGQLGSCHPLTGDCINQEPKDSSPAEECDDCDSCVMTLLNDLATMGE
QLRLVKSQLQGLSASAGLLEQMRHMETQAKDLRNQLLNYRSAISNHGSKIEGLERELT
DLNQEFETLQEKAQVNSRKAQTLNNNVNRATQSAKELDVKIKNVIRNVHILLKQISGT
DGEGNNVPSGDFSREWAEAQRMMRELRNRNFGKHLREAEADKRESQLLLNRIRTWQKT
HQGENNGLANSIRDSLNEYEAKLSDLRARLQEAAAQAKQANGLNQENERALGAIQRQV
KEINSLQSDFTKYLTTADSSLLQTNIALQLMEKSQKEYEKLAASLNEARQELSDKVRE
LSRSAGKTSLVEEAEKHARSLQELAKQLEEIKRNASGDELVRCAVDAATAYENILNAI
KAAEDAANRAASASESALQTVIKEDLPRKAKTLSSNSDKLLNEAKMTQKKLKQEVSPA
LNNLQQTLNIVTVQKEVIDTNLTTLRDGLHGIQRGDIDAMISSAKSMVRKANDITDEV
LDGLNPIQTDVERIKDTYGRTQNEDFKKALTDADNSVNKLTNKLPDLWRKIESINQQL
LPLGNISDNMDRIRELIQQARDAASKVAVPMRFNGKSGVEVRLPNDLEDLKGYTSLSL
FLQRPNSRENGGTENMFVMYLGNKDASRDYIGMAVVDGQLTCVYNLGDREAELQVDQI
LTKSETKEAVMDRVKFQRIYQFARLNYTKGATSSKPETPGVYDMDGRNSNTLLNLDPE
NVVFYVGGYPPDFKLPSRLSFPPYKGCIELDDLNENVLSLYNFKKTFNLNTTEVEPCR
RRKEESDKNYFEGTGYARVPTQPHAPIPTFGQTIQTTVDRGLLFFAENGDRFISLNIE
DGKLMVRYKLNSELPKERGVGDAINNGRDHSIQIKIGKLQKRMWINVDVQNTIIDGEV
FDFSTYYLGGIPIAIRERFNISTPAFRGCMKNLKKTSGVVRLNDTVGVTKKCSEDWKL
VRSASFSRGGQLSFTDLGLPPTDHLQASFGFQTFQPSGILLDHQTWTRNLQVTLEDGY
IELSTSDSGGPIFKSPQTYMDGLLHYVSVISDNSGLRLLIDDQLLRNSKRLKHISSSR
QSLRLGGSNFEGCISNVFVQRLSLSPEVLDLTSNSLKRDVSLGGCSLNKPPFLMLLKG
STRFNKTKTFRINQLLQDTPVASPRSVKVWQDACSPLPKTQANHGALQFGDIPTSHLL
FKLPQELLKPRSQFAVDMQTTSSRGLVFHTGTKNSFMALYLSKGRLVFALGTDGKKLR
IKSKEKCNDGKWHTVVFGHDGEKGRLVVDGLRAREGSLPGNSTISIRAPVYLGSPPSG
KPKSLPTNSFVGCLKNFQLDSKPLYTPSSSFGVSSCLGGPLEKGIYFSEEGGHVVLAH
SVLLGPEFKLVFSIRPRSLTGILIHIGSQPGKHLCVYLEAGKVTASMDSGAGGTSTSV
TPKQSLCDGQWHSVAVTIKQHILHLELDTDSSYTAGQIPFPPASTQEPLHLGGAPANL
TTLRIPVWKSFFGCLRNIHVNHIPVPVTEALEVQGPVSLNGCPDQ"
sig_peptide 1..60
/gene="LamA3"
repeat_region 136..603
/gene="LamA3"
/note="EGF repeats, domain IIIa"
repeat_region 604..2379
/gene="LamA3"
/note="heptad repeats, domain I/II"
3'UTR 5140..5433
/gene="LamA3"
polyA_site 5433
/gene="LamA3"
BASE COUNT 1618 a 1233 c 1338 g 1244 t
ORIGIN
1 atgggatggc tgtggatctt tggggcagcc ctggggcagt gtctgggcta cagttcacag
61 cagcaaaggg tgccatttct tcagcctccc ggtcaaagtc aactgcaagc gagttatgtg
121 gagtttagac ccagccaggg ttgtagccct ggatactatc gggatcataa aggcttgtat
181 accggacggt gtgttccctg caattgcaac ggacattcaa atcaatgcca ggatggctca
241 ggcatatgtg ttaactgtca gcacaacacc gcgggagagc actgtgaacg ctgccaggag
301 ggctactatg gcaacgccgt ccacggatcc tgcagggcct gcccatgtcc tcacactaac
361 agctttgcca ctggctgtgt ggtgaatggg ggagacgtgc ggtgctcctg caaagctggg
421 tacacaggaa cacagtgtga aaggtgtgca ccgggatatt tcgggaatcc ccagaaattc
481 ggaggtagct gccaaccatg cagttgtaac agcaatggcc agctgggcag ctgtcatccc
541 ctgactggag actgcataaa ccaagaaccc aaagatagca gccctgcaga agaatgtgat
601 gattgcgaca gctgtgtgat gaccctcctg aacgacctgg ccaccatggg cgagcagctc
661 cgcctggtca agtctcagct gcagggcctg agtgccagcg cagggcttct ggagcagatg
721 aggcacatgg agacccaggc caaggacctg aggaatcagt tgctcaacta ccgttctgcc
781 atttcaaatc atggatcaaa aatagaaggc ctggaaagag aactgactga tttgaatcaa
841 gaatttgaga ctttgcaaga aaaggctcaa gtaaattcca gaaaagcaca aacattaaac
901 aacaatgtta atcgggcaac acaaagcgca aaagaactgg atgtgaagat taaaaatgtc
961 atccggaatg tgcacattct tttaaagcag atctctggga cagatggaga gggaaacaac
1021 gtgccttcag gtgacttttc cagagagtgg gctgaagccc agcgcatgat gagggaactg
1081 cggaacagga actttggaaa gcacctcaga gaagcagaag ctgataaaag ggagtcgcag
1141 ctcttgctga accggataag gacctggcag aaaacccacc agggggagaa caatgggctt
1201 gctaacagta tccgggattc tttaaatgaa tacgaagcca aactcagtga ccttcgtgct
1261 cggctgcagg aggcagctgc ccaagccaag caggcaaatg gcttgaacca agaaaacgag
1321 agagctttgg gagccattca gagacaagtg aaagaaataa attccctgca gagtgatttc
1381 accaagtatc taaccactgc agactcatct ttgttgcaaa ccaacattgc gctgcagctg
1441 atggagaaaa gccagaagga atatgaaaaa ttagctgcca gtttaaatga agcaagacaa
1501 gaactaagtg acaaagtaag agaactttcc agatctgctg gcaaaacatc ccttgtggag
1561 gaggcagaaa agcacgcgcg gtccttacaa gagctggcaa agcagctgga agagatcaag
1621 agaaacgcca gcggggatga gctggtgcgc tgtgctgtgg atgccgccac cgcctacgag
1681 aacatcctca atgccatcaa agcggccgag gacgcagcca acagggctgc cagtgcatct
1741 gaatctgccc tccagacagt gataaaggaa gatctgccaa gaaaagctaa aaccctgagt
1801 tccaacagtg ataaactgtt aaatgaagcc aagatgacac aaaagaagct aaagcaagaa
1861 gtcagtccag ctctcaacaa cctacagcaa accctgaata ttgtgacagt tcagaaagaa
1921 gtgatagaca ccaatctcac aactctccga gatggtcttc atgggataca gagaggtgat
1981 attgatgcta tgatcagtag tgcaaagagc atggtcagaa aggccaacga catcacagat
2041 gaggttctgg atgggctcaa ccccatccag acagatgtgg aaagaattaa ggacacctat
2101 gggaggacac agaacgaaga cttcaaaaag gctctgactg atgcagataa ctcggtgaat
2161 aagttaacca acaaactacc tgatctttgg cgcaagattg aaagtatcaa ccaacagctg
2221 ttgcccttgg gaaacatctc tgacaacatg gacagaatac gagaactaat tcagcaggcc
2281 agagatgctg ccagtaaggt tgctgtcccc atgaggttca atggtaaatc tggagtcgaa
2341 gtccgactgc caaatgacct ggaagatttg aaaggatata catctctgtc cttgtttctc
2401 caaaggccca actcaagaga aaatgggggt actgagaata tgtttgtgat gtaccttgga
2461 aataaagatg cctcccggga ctacatcggc atggcagttg tggatggcca gctcacctgt
2521 gtctacaacc tgggggaccg tgaggctgaa ctccaagtgg accagatctt gaccaagagt
2581 gagactaagg aggcagttat ggatcgggtg aaatttcaga gaatttatca gtttgcaagg
2641 cttaattaca ccaaaggagc cacatccagt aaaccagaaa cacccggagt ctatgacatg
2701 gatggtagaa atagcaatac actccttaat ttggatcctg aaaatgttgt attttatgtt
2761 ggaggttacc cacctgattt taaacttccc agtcgactaa gtttccctcc atacaaaggt
2821 tgtattgaat tagatgacct caatgaaaat gttctgagct tgtacaactt caaaaaaaca
2881 ttcaatctca acacaactga agtggagcct tgtagaagga ggaaggaaga gtcagacaaa
2941 aattattttg aaggtacggg ctatgctcga gttccaactc aaccacatgc tcccatccca
3001 acctttggac agacaattca gaccaccgtg gatagaggct tgctgttctt tgcagaaaac
3061 ggggatcgct tcatatctct aaatatagaa gatggcaagc tcatggtgag atacaaactg
3121 aattcagagc taccaaaaga gagaggagtt ggagacgcca taaacaacgg cagagaccat
3181 tcgattcaga tcaaaattgg aaaactccaa aagcgtatgt ggataaatgt ggacgttcaa
3241 aacactataa ttgatggtga agtatttgat ttcagcacat attatctggg aggaattcca
3301 attgcaatca gggaaagatt taacatttct acgcctgctt tccgaggctg catgaaaaat
3361 ttgaagaaaa ccagtggtgt cgttagattg aatgatactg tgggagtaac caaaaagtgc
3421 tcggaagact ggaagcttgt gcgatctgcc tcattctcca gaggaggaca attgagtttc
3481 actgatttgg gcttaccacc tactgaccac ctccaggcct catttggatt tcagaccttt
3541 caacccagtg gcatattatt agatcatcag acatggacaa ggaacctgca ggtcactctg
3601 gaagatggtt acattgaatt gagcaccagc gatagcggcg gcccaatttt taaatctcca
3661 cagacgtata tggatggttt actgcattat gtatctgtaa taagcgacaa ctctggacta
3721 cggcttctca tcgatgacca gcttctgaga aatagcaaaa ggctaaaaca catttcaagt
3781 tcccggcagt ctctgcgtct gggcgggagc aattttgagg gttgtattag caatgttttt
3841 gtccagaggt tatcactgag tcctgaagtc ctagatttga ccagtaactc tctcaagaga
3901 gatgtgtccc tgggaggctg cagtttaaac aaaccacctt ttctaatgtt gcttaaaggt
3961 tctaccaggt ttaacaagac caagactttt cgtatcaacc agctgttgca ggacacacca
4021 gtggcctccc caaggagcgt gaaggtgtgg caagatgctt gctcaccact tcccaagacc
4081 caggccaatc atggagccct ccagtttggg gacattccca ccagccactt gctattcaag
4141 cttcctcagg agctgctgaa acccaggtca cagtttgctg tggacatgca gacaacatcc
4201 tccagaggac tggtgtttca cacgggcact aagaactcct ttatggctct ttatctttca
4261 aaaggacgtc tggtctttgc actggggaca gatgggaaaa aattgaggat caaaagcaag
4321 gagaaatgca atgatgggaa atggcacacg gtggtgtttg gccatgatgg ggaaaagggg
4381 cgcttggttg tggatggact gagggcccgg gagggaagtt tgcctggaaa ctccaccatc
4441 agcatcagag cgccagttta cctgggatca cctccatcag ggaaaccaaa gagcctcccc
4501 acaaacagct ttgtgggatg cctgaagaac tttcagctgg attcaaaacc cttgtatacc
4561 ccttcttcaa gcttcggggt gtcttcctgc ttgggtggtc ctttggagaa aggcatttat
4621 ttctctgaag aaggaggtca tgtcgtcttg gctcactctg tattgttggg gccagaattt
4681 aagcttgttt tcagcatccg cccaagaagt ctcactggga tcctaataca catcggaagt
4741 cagcccggga agcacttatg tgtttacctg gaggcaggaa aggtcacggc ctctatggac
4801 agtggggcag gtgggacctc aacgtcggtc acaccaaagc agtctctgtg tgatggacag
4861 tggcactcgg tggcagtcac cataaaacaa cacatcctgc acctggaact ggacacagac
4921 agtagctaca cagctggaca gatccccttc ccacctgcca gcactcaaga gccactacac
4981 cttggaggtg ctccagccaa tttgacgaca ctgaggatcc ctgtgtggaa atcattcttt
5041 ggctgtctga ggaatattca tgtcaatcac atccctgtcc ctgtcactga agccttggaa
5101 gtccaggggc ctgtcagtct gaatggttgt cctgaccagt aacccaagcc tatttcacag
5161 caaggaaatt caccttcaaa agcactgatt acccaatgca cctccctccc cagctcgaga
5221 tcattcttca attaggacac aaaccagaca ggtttaatag cgaatctaat tttgaattct
5281 gaccatggat acccatcact ttggcattca gtgctacatg tgtattttat ataaaaatcc
5341 catttcttga agataaaaaa attgttattc aaattgttat gcacagaatg tttttggtaa
5401 tattaatttc cactaaaaaa ttaaatgtct ttt
//