LOCUS HUMFRAPX 7943 bp mRNA linear HUM 31-DEC-1994
DEFINITION Human FKBP-rapamycin associated protein (FRAP) mRNA, complete cds.
ACCESSION L34075
VERSION L34075.1
KEYWORDS FKBP-rapamycin associated protein; phosphatidylinositol 3-kinase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 7943)
AUTHORS Brown,E.J., Albers,M.W., Shin,T.B., Ichikawa,K., Keith,C.T.,
Lane,W.S. and Schreiber,S.L.
TITLE A mammalian protein targeted by G1-arresting rapamycin-receptor
complex
JOURNAL Nature 369 (6483), 756-758 (1994)
PUBMED 8008069
COMMENT Original source text: Homo sapiens (tissue library: lambda ZAPII)
cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..7943
/db_xref="H-InvDB:HIT000193125_01"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="jurkat"
/cell_type="T-cell"
/tissue_lib="lambda ZAPII"
gene 1..7943
/gene="FRAP"
5'UTR 1..79
/gene="FRAP"
CDS 80..7729
/gene="FRAP"
/function="acts as the target for the cell-cycle arrest
and immunosuppressive effects of the FKBP12-rapamycin
complex"
/note="homologue of phosphatidylinositol-3'-kinase (PI3K),
TOR1, and TOR2 genes"
/codon_start=1
/product="FKBP-rapamycin associated protein"
/protein_id="AAA58486.1"
/translation="MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKEL
QHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNAT
RIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADR
NEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLI
LTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISS
MEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLL
GYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTI
LNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKV
YLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPM
LAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLA
SPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAAR
TCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDER
FDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTE
LEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNV
LATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVE
PYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASA
VSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVV
QAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPY
MDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSP
GRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESL
DFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRI
NHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVST
INLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDN
GIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYA
MKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLH
QQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLAL
HQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPE
RREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLAL
AHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQ
QAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDR
SWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTA
STEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNL
QDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIH
QLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEEL
IRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAY
GRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLM
CRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHE
DLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHA
LIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLK
SPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEV
AMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFV
YDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIH
SFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENL
CQCYIGWCPFW"
3'UTR 7730..7943
/gene="FRAP"
BASE COUNT 1972 a 2103 c 2094 g 1774 t
ORIGIN
1 acggggcctg aagcggcggt accggtgctg gcggcggcag ctgaggcctt ggccgaagcc
61 gcgcgaacct cagggcaaga tgcttggaac cggacctgcc gccgccacca ccgctgccac
121 cacatctagc aatgtgagcg tcctgcagca gtttgccagt ggcctaaaga gccggaatga
181 ggaaaccagg gccaaagccg ccaaggagct ccagcactat gtcaccatgg aactccgaga
241 gatgagtcaa gaggagtcta ctcgcttcta tgaccaactg aaccatcaca tttttgaatt
301 ggtttccagc tcagatgcca atgagaggaa aggtggcatc ttggccatag ctagcctcat
361 aggagtggaa ggtgggaatg ccacccgaat tggcagattt gccaactatc ttcggaacct
421 cctcccctcc aatgacccag ttgtcatgga aatggcatcc aaggccattg gccgtcttgc
481 catggcaggg gacactttta ccgctgagta cgtggaattt gaggtgaagc gagccctgga
541 atggctgggt gctgaccgca atgagggccg gagacatgca gctgtcctgg ttctccgtga
601 gctggccatc agcgtcccta ccttcttctt ccagcaagtg caacccttct ttgacaacat
661 ttttgtggcc gtgtgggacc ccaaacaggc catccgtgag ggagctgtag ccgcccttcg
721 tgcctgtctg attctcacaa cccagcgtga gccgaaggag atgcagaagc ctcagtggta
781 caggcacaca tttgaagaag cagagaaggg atttgatgag accttggcca aagagaaggg
841 catgaatcgg gatgatcgga tccatggagc cttgttgatc cttaacgagc tggtccgaat
901 cagcagcatg gagggagagc gtctgagaga agaaatggaa gaaatcacac agcagcagct
961 ggtacacgac aagtactgca aagatctcat gggcttcgga acaaaacctc gtcacattac
1021 ccccttcacc agtttccagg ctgtacagcc ccagcagtca aatgccttgg tggggctgct
1081 ggggtacagc tctcaccaag gcctcatggg atttgggacc tcccccagtc cagctaagtc
1141 caccctggtg gagagccggt gttgcagaga cttgatggag gagaaatttg atcaggtgtg
1201 ccagtgggtg ctgaaatgca ggaatagcaa gaactcgctg atccaaatga caatccttaa
1261 tttgttgccc cgcttggctg cattccgacc ttctgccttc acagataccc agtatctcca
1321 agataccatg aaccatgtcc taagctgtgt caagaaggag aaggaacgta cagcggcctt
1381 ccaagccctg gggctacttt ctgtggctgt gaggtctgag tttaaggtct atttgcctcg
1441 cgtgctggac atcatccgag cggccctgcc cccaaaggac ttcgcccata agaggcagaa
1501 ggcaatgcag gtggacgcca cagtcttcac ttgcatcagc atgctggctc gagcaatggg
1561 gccaggcatc cagcaggata tcaaggagct gctggagccc atgctggcag tgggactaag
1621 ccctgccctc actgcagtgc tctacgacct gagccgtcag attccacagc taaagaagga
1681 cattcaagat gggctactga aaatgctgtc cctggtcctt atgcacaaac cccttcgcca
1741 cccaggcatg cccaagggcc tggcccatca gctggcctct cctggcctca cgaccctccc
1801 tgaggccagc gatgtgggca gcatcactct tgccctccga acgcttggca gctttgaatt
1861 tgaaggccac tctctgaccc aatttgttcg ccactgtgcg gatcatttcc tgaacagtga
1921 gcacaaggag atccgcatgg aggctgcccg cacctgctcc cgcctgctca caccctccat
1981 ccacctcatc agtggccatg ctcatgtggt tagccagacc gcagtgcaag tggtggcaga
2041 tgtgcttagc aaactgctcg tagttgggat aacagatcct gaccctgaca ttcgctactg
2101 tgtcttggcg tccctggacg agcgctttga tgcacacctg gcccaggcgg agaacttgca
2161 ggccttgttt gtggctctga atgaccaggt gtttgagatc cgggagctgg ccatctgcac
2221 tgtgggccga ctcagtagca tgaaccctgc ctttgtcatg cctttcctgc gcaagatgct
2281 catccagatt ttgacagagt tggagcacag tgggattgga agaatcaaag agcagagtgc
2341 ccgcatgctg gggcacctgg tctccaatgc cccccgactc atccgcccct acatggagcc
2401 tattctgaag gcattaattt tgaaactgaa agatccagac cctgatccaa acccaggtgt
2461 gatcaataat gtcctggcaa caataggaga attggcacag gttagtggcc tggaaatgag
2521 gaaatgggtt gatgaacttt ttattatcat catggacatg ctccaggatt cctctttgtt
2581 ggccaaaagg caggtggctc tgtggaccct gggacagttg gtggccagca ctggctatgt
2641 agtagagccc tacaggaagt accctacttt gcttgaggtg ctactgaatt ttctgaagac
2701 tgagcagaac cagggtacac gcagagaggc catccgtgtg ttagggcttt taggggcttt
2761 ggatccttac aagcacaaag tgaacattgg catgatagac cagtcccggg atgcctctgc
2821 tgtcagcctg tcagaatcca agtcaagtca ggattcctct gactatagca ctagtgaaat
2881 gctggtcaac atgggaaact tgcctctgga tgagttctac ccagctgtgt ccatggtggc
2941 cctgatgcgg atcttccgag accagtcact ctctcatcat cacaccatgg ttgtccaggc
3001 catcaccttc atcttcaagt ccctgggact caaatgtgtg cagttcctgc cccaggtcat
3061 gcccacgttc cttaatgtca ttcgagtctg tgatggggcc atccgggaat ttttgttcca
3121 gcagctggga atgttggtgt cctttgtgaa gagccacatc agaccttata tggatgaaat
3181 agtcaccctc atgagagaat tctgggtcat gaacacctca attcagagca cgatcattct
3241 tctcattgag caaattgtgg tagctcttgg gggtgaattt aagctctacc tgccccagct
3301 gatcccacac atgctgcgtg tcttcatgca tgacaacagc ccaggccgca ttgtctctat
3361 caagttactg gctgcaatcc agctgtttgg cgccaacctg gatgactacc tgcatttact
3421 gctgcctcct attgttaagt tgtttgatgc ccctgaagct ccactgccat ctcgaaaggc
3481 agcgctagag actgtggacc gcctgacgga gtccctggat ttcactgact atgcctcccg
3541 gatcattcac cctattgttc gaacactgga ccagagccca gaactgcgct ccacagccat
3601 ggacacgctg tcttcacttg tttttcagct ggggaagaag taccaaattt tcattccaat
3661 ggtgaataaa gttctggtgc gacaccgaat caatcatcag cgctatgatg tgctcatctg
3721 cagaattgtc aagggataca cacttgctga tgaagaggag gatcctttga tttaccagca
3781 tcggatgctt aggagtggcc aaggggatgc attggctagt ggaccagtgg aaacaggacc
3841 catgaagaaa ctgcacgtca gcaccatcaa cctccaaaag gcctggggcg ctgccaggag
3901 ggtctccaaa gatgactggc tggaatggct gagacggctg agcctggagc tgctgaagga
3961 ctcatcatcg ccctccctgc gctcctgctg ggccctggca caggcctaca acccgatggc
4021 cagggatctc ttcaatgctg catttgtgtc ctgctggtct gaactgaatg aagatcaaca
4081 ggatgagctc atcagaagca tcgagttggc cctcacctca caagacatcg ctgaagtcac
4141 acagaccctc ttaaacttgg ctgaattcat ggaacacagt gacaagggcc ccctgccact
4201 gagagatgac aatggcattg ttctgctggg tgagagagct gccaagtgcc gagcatatgc
4261 caaagcacta cactacaaag aactggagtt ccagaaaggc cccacccctg ccattctaga
4321 atctctcatc agcattaata ataagctaca gcagccggag gcagcggccg gagtgttaga
4381 atatgccatg aaacactttg gagagctgga gatccaggct acctggtatg agaaactgca
4441 cgagtgggag gatgcccttg tggcctatga caagaaaatg gacaccaaca aggacgaccc
4501 agagctgatg ctgggccgca tgcgctgcct cgaggccttg ggggaatggg gtcaactcca
4561 ccagcagtgc tgtgaaaagt ggaccctggt taatgatgag acccaagcca agatggcccg
4621 gatggctgct gcagctgcat ggggtttagg tcagtgggac agcatggaag aatacacctg
4681 tatgatccct cgggacaccc atgatggggc attttataga gctgtgctgg cactgcatca
4741 ggacctcttc tccttggcac aacagtgcat tgacaaggcc agggacctgc tggatgctga
4801 attaactgca atggcaggag agagttacag tcgggcatat ggggccatgg tttcttgcca
4861 catgctgtcc gagctggagg aggttatcca gtacaaactt gtccccgagc gacgagagat
4921 catccgccag atctggtggg agagactgca gggctgccag cgtatcgtag aggactggca
4981 gaaaatcctt atggtgcggt cccttgtggt cagccctcat gaagacatga gaacctggct
5041 caagtatgca agcctgtgcg gcaagagtgg caggctggct cttgctcata aaactttagt
5101 gttgctcctg ggagttgatc cgtctcggca acttgaccat cctctgccaa cagttcaccc
5161 tcaggtgacc tatgcctaca tgaaaaacat gtggaagagt gcccgcaaga tcgatgcctt
5221 ccagcacatg cagcattttg tccagaccat gcagcaacag gcccagcatg ccatcgctac
5281 tgaggaccag cagcataagc aggaactgca caagctcatg gcccgatgct tcctgaaact
5341 tggagagtgg cagctgaatc tacagggcat caatgagagc acaatcccca aagtgctgca
5401 gtactacagc gccgccacag agcacgaccg cagctggtac aaggcctggc atgcgtgggc
5461 agtgatgaac ttcgaagctg tgctacacta caaacatcag aaccaagccc gcgatgagaa
5521 gaagaaactg cgtcatgcca gcggggccaa catcaccaac gccaccactg ccgccaccac
5581 ggccgccact gccaccacca ctgccagcac cgagggcagc aacagtgaga gcgaggccga
5641 gagcaccgag aacagcccca ccccatcgcc gctgcagaag aaggtcactg aggatctgtc
5701 caaaaccctc ctgatgtaca cggtgcctgc cgtccagggc ttcttccgtt ccatctcctt
5761 gtcacgaggc aacaacctcc aggatacact cagagttctc accttatggt ttgattatgg
5821 tcactggcca gatgtcaatg aggccttagt ggagggggtg aaagccatcc agattgatac
5881 ctggctacag gttatacctc agctcattgc aagaattgat acgcccagac ccttggtggg
5941 acgtctcatt caccagcttc tcacagacat tggtcggtac cacccccagg ccctcatcta
6001 cccactgaca gtggcttcta agtctaccac gacagcccgg cacaatgcag ccaacaagat
6061 tctgaagaac atgtgtgagc acagcaacac cctggtccag caggccatga tggtgagcga
6121 ggagctgatc cgagtggcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc
6181 atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt
6241 gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta
6301 tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt
6361 caaggacctc acccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaagca
6421 gctgcctcag ctcacatcct tagagctgca atatgtttcc ccaaaacttc tgatgtgccg
6481 ggaccttgaa ttggctgtgc caggaacata tgaccccaac cagccaatca ttcgcattca
6541 gtccatagca ccgtctttgc aagtcatcac atccaagcag aggccccgga aattgacact
6601 tatgggcagc aacggacatg agtttgtttt ccttctaaaa ggccatgaag atctgcgcca
6661 ggatgagcgt gtgatgcagc tcttcggcct ggttaacacc cttctggcca atgacccaac
6721 atctcttcgg aaaaacctca gcatccagag atacgctgtc atccctttat cgaccaactc
6781 gggcctcatt ggctgggttc cccactgtga cacactgcac gccctcatcc gggactacag
6841 ggagaagaag aagatccttc tcaacatcga gcatcgcatc atgttgcgga tggctccgga
6901 ctatgaccac ttgactctga tgcagaaggt ggaggtgttt gagcatgccg tcaataatac
6961 agctggggac gacctggcca agctgctgtg gctgaaaagc cccagctccg aggtgtggtt
7021 tgaccgaaga accaattata cccgttcttt agcggtcatg tcaatggttg ggtatatttt
7081 aggcctggga gatagacacc catccaacct gatgctggac cgtctgagtg ggaagatcct
7141 gcacattgac tttggggact gctttgaggt tgctatgacc cgagagaagt ttccagagaa
7201 gattccattt agactaacaa gaatgttgac caatgctatg gaggttacag gcctggatgg
7261 caactacaga atcacatgcc acacagtgat ggaggtgctg cgagagcaca aggacagtgt
7321 catggccgtg ctggaagcct ttgtctatga ccccttgctg aactggaggc tgatggacac
7381 aaataccaaa ggcaacaagc gatcccgaac gaggacggat tcctactctg ctggccagtc
7441 agtcgaaatt ttggacggtg tggaacttgg agagccagcc cataagaaaa cggggaccac
7501 agtgccagaa tctattcatt ctttcattgg agacggtttg gtgaaaccag aggccctaaa
7561 taagaaagct atccagatta ttaacagggt tcgagataag ctcactggtc gggacttctc
7621 tcatgatgac actttggatg ttccaacgca agttgagctg ctcatcaaac aagcgacatc
7681 ccatgaaaac ctctgccagt gctatattgg ctggtgccct ttctggtaac tggaggccca
7741 gatgtgccca tcacgttttt tctgaggctt ttgtacttta gtaaatgctt ccactaaact
7801 gaaaccatgg tgagaaagtt tgactttgtt aaatattttg aaatgtaaat gaaaagaagt
7861 actgtatatt aaaagttggt ttgaaccaac tttctagctg ctgttgaaga atatattgtc
7921 agaaacacaa ggcttgattt ggt
//