LOCUS HUMAMII 4917 bp mRNA linear HUM 13-MAR-1996
DEFINITION Homo sapiens alpha mannosidase II isozyme mRNA, complete cds.
ACCESSION L28821
VERSION L28821.1
KEYWORDS alpha mannosidase IIx.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4917)
AUTHORS Misago,M., Liao,Y.F., Kudo,S., Eto,S., Mattei,M.G., Moremen,K.W.
and Fukuda,M.N.
TITLE Molecular cloning and expression of cDNAs encoding human
alpha-mannosidase II and a previously unrecognized
alpha-mannosidase IIx isozyme
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 92 (25), 11766-11770 (1995)
PUBMED 8524845
COMMENT Original source text: Homo sapiens (tissue library: ATCC HTB 72)
melanoma cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..4917
/db_xref="H-InvDB:HIT000192755"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="SK-Mel-28"
/cell_type="myeloma cell"
/tissue_type="melanoma"
/tissue_lib="ATCC HTB 72"
5'UTR 1..68
CDS 69..2459
/codon_start=1
/product="alpha mannosidase II isozyme"
/protein_id="AAA92022.1"
/translation="MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPTRHQNGGNFPR
SQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPAMLPYYTVNGSWVVPP
EPRPSFFSISPQDCQFALGGRGQKPELQMLTVSEELPFDNVDGGVWRQGFDISYDPHD
WDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSF
FAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLER
NLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQ
TWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAIT
EANVAERAALLLDQYRKKSQLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFFN
SRPNLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYY
TSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAGRYPLSDFTLLTEARRTLGLF
QHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYLVLGDKETYHFDPEAPFLQ
VDDTRLSHDALPERTVIQLDSSPRFVVLFNPLEQERFSMVSLLVNSPRVRVLSEEGQP
LAVQISAHWSSATEAVPDVYQVSVPVRLPALGLGVLQLQLGLDGHRTLPSSVRIYLHG
RQLSVSRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKGSGLCFLAEHPKGG"
3'UTR 2460..4917
regulatory 4895..4900
/regulatory_class="polyA_signal_sequence"
BASE COUNT 1057 a 1413 c 1376 g 1071 t
ORIGIN
1 ggcagctcgg ccgactgggc ccggagcggc gcggaggccg ggcgctgacg gtgtgtgtgg
61 aggccagtat gaagctgaaa aagcaggtga cagtgtgtgg ggctgccatc ttctgtgtgg
121 cagtcttctc gctctacctc atgctggacc gagtgcaaca cgatcccacc cgacaccaga
181 atggtgggaa cttcccccgg agccaaattt ctgtgctgca gaaccgcatt gagcagctgg
241 agcagctttt ggaggagaac catgagatta tcagccatat caaggactcc gtgctggagc
301 tgacagccaa cgcagagggc ccgcccgcca tgctgcccta ctacacggtc aatggctcct
361 gggtggtgcc accggagccc cggcccagct tcttctccat ctccccgcag gactgccagt
421 ttgctttggg gggccggggt cagaagccag agctgcagat gctcactgtg tcggaggagc
481 tgccgtttga caacgtggat ggtggtgtgt ggaggcaagg cttcgacatc tcctacgacc
541 cgcacgactg ggatgctgaa gacctgcagg tgtttgtggt gccccactct cacaatgacc
601 caggctggat caagaccttt gacaagtact acacagagca gacccaacac atcctcaata
661 gcatggtgtc taagctgcag gaggaccccc ggcggcgctt cctctgggca gaggtctcct
721 tcttcgccaa gtggtgggac aacatcaatg tccaaaagag agcggcagtc cgaaggctgg
781 tgggaaacgg gcagctggag attgcgacag gaggctgggt gatgccagat gaggccaatt
841 cccactactt tgcattgatt gaccagctca tcgaaggaca ccagtggctg gagagaaatc
901 ttggtgcaac cccccgctct ggctgggcag tggacccctt tggatacagc tccaccatgc
961 cttacctgct gcgccgtgcc aacctcacca gcatgctgat tcagagagtg cactatgcca
1021 tcaagaagca ctttgctgcc acccacagcc tagagttcat gtggaggcag acatgggact
1081 cggactccag cacagacatc ttctgtcaca tgatgccctt ctacagctat gacgtccccc
1141 atacctgtgg cccagatccc aagatctgct gccaatttga tttcaaacgc ctgcctggtg
1201 ggcgcatcaa ctgcccttgg aaggtgccac cccgggccat cacagaggcc aacgtggcag
1261 agagggcagc cctgcttctg gaccaatacc ggaagaagtc ccagctgttc cgaagcaacg
1321 tcctcctggt gcctcttgga gatgacttcc gatatgacaa gccccaggag tgggatgccc
1381 agttcttcaa ctaccaacgg ctctttgact tcttcaacag caggcctaac ctccatgtgc
1441 aggcccagtt tggcactctt tctgactatt ttgatgccct gtacaagagg acaggggtgg
1501 agccaggggc ccggcctcca gggtttcctg tgctgagcgg ggatttcttc tcctatgcgg
1561 accgggagga tcattactgg acaggctatt acacttcccg gcccttctac aagagcttag
1621 accgagtcct ggaagcccac ctgcgggggg cagaggttct gtacagcctg gctgcagctc
1681 acgctcgccg ctctggtctg gctggccggt acccactgtc tgatttcacc ctcctgacgg
1741 aagctcggcg cacattgggg ctcttccagc atcacgatgc catcactggc acggccaagg
1801 aggctgtggt ggtggactat ggggtcaggc ttctgcgctc ccttgtcaac ctgaagcagg
1861 tcatcattca tgcagcccac tatctggtgc tgggggacaa ggagacctac cactttgacc
1921 ctgaggcgcc cttcctccaa gtggatgaca ctcgcttaag tcacgacgcc ctcccagagc
1981 gcacggtgat ccagctggat tcctcgccca ggtttgtggt cctattcaac ccactggaac
2041 aggagcgatt cagcatggtg tccctgctgg tcaactctcc ccgcgtgcgt gtcctttcgg
2101 aggagggtca gcccctggcc gtgcagatca gcgcacactg gagctctgcc accgaggcgg
2161 tccctgacgt ctaccaggtg tctgtgcctg tccgcctgcc agccctgggc ctgggcgtgc
2221 tgcagctaca gctgggcctg gatgggcacc gcacgctgcc ctcctctgtg cgcatctacc
2281 tgcacggccg gcagctgtcc gtcagcaggc acgaagcgtt tcctctccgt gtcattgact
2341 ctggcaccag cgacttcgcc ctcagcaacc gctacatgca ggtctggttc tcaggcctta
2401 ctgggctcct caaggggtca gggctgtgtt ttttggcaga gcatccgaag ggtggatgag
2461 gagcacgagc agcaggtgga catgcaggtc cttgtctatg gcacccgtac gtccaaagac
2521 aagagtggag cctacctctt cctgcccgat ggcgaggcaa gccctacgtc cccaaggagc
2581 cccccgtgct gcgtgtcact gaaggccctt tcttctcaga ggtggttgcg tactatgagc
2641 acattcacca ggcggtccgg ctttacaatc tgccaggggt ggaggggctg tctctggaca
2701 tatcatccct ggtggacatc cgggactacg tcaacaagga gctggccctg cacatccata
2761 cagacatcga cagccagggt gcagccccga cggtatctga agaagctccc cctccaggcc
2821 aacttctacc ccatgccagt catggcctat atccaggacg cacagaagcg cctcacgctg
2881 cacactgccc aggccctggg tgtctctagc ctcaaagatg gccagctgga ggtgatcttg
2941 gaccggcggc tgatgcagga tgacaaccgg ggcctaggcc aagggctcaa ggacaacaag
3001 agaacctgca accgtttccg cctcctgcta gagcggcgaa ccgtgggcag tgaggtccaa
3061 gatagccact ctaccagcta cccatccctc ctcagccacc tgacctccat gtacctgaac
3121 gccccggcgc tcgctctgcc tgtagccagg atgcagctcc caggccctgg tctgcgctca
3181 tttcatcctc tggcttcctc actgccctgt gacttccacc tgctcaacct acgtacgctc
3241 caggctgagg aggacaccct accctcggcg gagaccgcac tcatcttaca ccgcaagggt
3301 tttgactgcg gcctggaggc caagaacttg ggcttcaact gcaccacaag ccaaggcaag
3361 gtagccctgg gcagcctttt ccatggcctg gatgtggtat tccttcagcc aacctccttg
3421 acgttactgt accctctggc ctccccgtcc aacagcactg acgtctattt ggagcccatg
3481 gagattgcta cctttcgcct ccgcttgggt tagggcttct tgtggcctga agagaaagtt
3541 cattcacaga gactgcctct taacatgaag atcattggac aagccacacg ggtatcccat
3601 cccgatctgc ctcccagaac tgtgacacac tgggctctgc cctcattttc tgtttattgc
3661 tgctgctgtg ttttcggcgc aacccacaaa cccagtgatg ggtaaatagg gcagacgcca
3721 gtgagatcag ggagagaagg cccttggtca gagtgggcag tgccaggctc tgctttgggt
3781 tgtgagtgga cacccaactg ggcacaggct caggcaccca tcctttttcc aaacagggat
3841 atagaagtgg tggaagcaga cagaagaggt aagggaggct aagtgggtaa cagcccagca
3901 tcagggtcac tgtggcaaca gcaggctcta ggggaatcct gtggttatgt agagactcca
3961 tgtcctggtg tgatgagcag gatcagagtg actctgggag gacaggggtg gggacccaga
4021 gttagcagtg gggatggagc agtagaagga atcactgttt ctcctaggag tctgaaggcc
4081 tcgctgcttt ctgtgatggc tttgcagtaa gtgccgcctg gcctgcatgc attggctaac
4141 aggctgcaga atggcaggaa ggactcgcta gagattgtca tggccagaga tcataggtca
4201 cttcaggtag caagacccct ggcaaactgg gcacttggcc tatgtactga tttgtgggat
4261 ggtggcaggg gtgtggggtc cttcaccctg cctgaattct ctttggcttc tgtgctctgt
4321 atgctgctgt ccccaagggc tctttcttat tatggcaggg agtggggatt ggtcctactt
4381 tctttctctg gaaaggaaag cctccaagac tccatgtgct tgggcagctt gagaaggcgt
4441 tcagcaccac gcctagcagg cagaccttga agcctcacct ttagtctatc tgcagaggta
4501 ttcagttcct ggcacagggg actaggggca tgtagagtat atgaggaggc agtatggctg
4561 tgcaggagcc ttcatttcag cttcaattaa tagggaagaa tttatgatag ctctatagat
4621 gctgaaaagg tatttcgtaa gatttaaaat ccatccctta ttaaaactct tagtaaatta
4681 agtctggaaa gaaacaccct aatctagata aaggtctgtt tcagaaacca acagtgatgg
4741 cattctaaag agtcagacgc cacaggcatt cccattaaag tcagaaacta gccaagggca
4801 agctattatt cagcagtgtc ccggcactac taacccctgc aacaagccag atgaggaaca
4861 taaggaagaa ttataattgt cattatttgt agacaataaa actgcctacc tgtaaaa
//