LOCUS HUMACYLCOA 4011 bp mRNA linear HUM 20-MAY-1999
DEFINITION Homo sapiens acyl-coenzyme A: cholesterol acyltransferase mRNA,
complete cds.
ACCESSION L21934
VERSION L21934.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4011)
AUTHORS Chang,C.C., Huh,H.Y., Cadigan,K.M. and Chang,T.Y.
TITLE Molecular cloning and functional expression of human acyl-coenzyme
A:cholesterol acyltransferase cDNA in mutant Chinese hamster ovary
cells
JOURNAL J. Biol. Chem. 268 (28), 20747-20755 (1993)
PUBMED 8407899
REFERENCE 2 (bases 1 to 4011)
AUTHORS Chang,C.C. and Chang,T.Y.
TITLE Direct Submission
JOURNAL Submitted (02-NOV-1993) Biochemistry Dept, Dartmouth Medical
School, Hanover, NH 03755, USA
REFERENCE 3 (bases 1 to 4011)
AUTHORS Chang,C.C. and Chang,T.Y.
TITLE Direct Submission
JOURNAL Submitted (20-MAY-1999) Biochemistry Dept, Dartmouth Medical
School, Hanover, NH 03755, USA
REMARK Sequence update by submitter
COMMENT On May 20, 1999 this sequence version replaced L21934.1.
FEATURES Location/Qualifiers
source 1..4011
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="THP-1 (Phorbol ester activated)"
/cell_type="macrophage"
CDS 1397..3049
/standard_name="ACAT"
/codon_start=1
/product="acyl-coenzyme A: cholesterol acyltransferase"
/protein_id="AAC37532.2"
/translation="MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDI
KQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEG
EKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVD
YIDEGRLVLEFSLLSYAFGKFPTVVWTWWIMFLSTFSVPYFLFQHWATGYSKSSHPLI
RSLFHGFLFMIFQIGVLGFGPTYVVLAYTLPPASRFIIIFEQIRFVMKAHSFVRENVP
RVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCF
FYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLTFFAFLHCWLNAF
AEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAML
AVFAVSAVVHEYALAVCLSFFYPVLFVLFMFFGMAFNFIVNDSRKKPIWNVLMWTSLF
LGNGVLLCFYSQEWYARRHCPLKNPTFLDYVRPRSWTCRYVF"
regulatory 3384..3389
/regulatory_class="polyA_signal_sequence"
regulatory 3962..3967
/regulatory_class="polyA_signal_sequence"
BASE COUNT 1056 a 793 c 838 g 1324 t
ORIGIN
1 gggtagagac ggggtttcac cgtgttagcc aggatggtct ggatctcctg acctcgtgat
61 ccacccacct cggcctccta aagtgctggg attacagaca tgagccaccg cgcccagccc
121 tattcatccc ttttcaaaag tcagacccta ggaagctgga gggaggtggg gcatggtttt
181 acagtgaatt tctgatttca ctcagggtga taaatcagac tcttggggaa gcgggtggtg
241 gctctggaca gcagcaggaa tggggatcca gttagcaaca aatccatgga cctatgacag
301 gctgaaagcc accccttctc catctttggg aggttgccaa tgtctgattt aacactatcc
361 aatgaatgat cattgaaagt aaaaaataac tatcaactag cagaaaatat aaatggtaag
421 cattagcaca tatttcacat gtttatattt ggctctcaga ttgacctata aaacaaagtc
481 tgggaaattc tatatgatcc tgaaaaaatg atacgctggt ctggatggta gaataagttg
541 gagaaatgtt taagccaaaa tgcagtctta ccaatgactt tttattttat tttattaatt
601 ttcaggattt ttggtataca ggtggttttt ggttacatgg aaaagttctt tactggtgat
661 ttctgagatt ttagttcacc ccttatcctg agcagtgtac actgttccca atatgtagcc
721 ttttatccct caccccctct aagttcaaga agactatggt cctgcagaaa gctttatatg
781 taattaacat atctttatct ttatctttat aggcagtaga ctcatctttt gaaacagatt
841 ccattaagag tgaatgtgta ccctccctct agcctttatt attactgttt ttgctattac
901 atgtgttagt gtatgtgaat ttaatgctta aaaatgtatc ccattggcta ctatggcaaa
961 aggttgactc ataagagttt agcacgggtt aagatctgaa agttttctcc cagcctctta
1021 tcactggcgc agacttcaca attcatggaa gccaccagtg agatgacatt gcctcaggca
1081 gttactattt ttatattcta taactcgagg agctcagggt ttcggaaatc attaaacttt
1141 ttttgtcctt ttaaagttgg agacagcaat tgtagacagc cttccagtgg gttatctttt
1201 tgtgtctcct tacctgtgga gaagcctatt agctgggata tgtagttaaa tagctatatt
1261 tatatatatc cagggcaccc cgaattcggg agagcttccc ggagtcgacc ttcctgctgg
1321 ctgctctgtg accgcttccc ggctctgccc tcttggccga agtgcccgct gccgggcgcg
1381 ggcctcagac aatacaatgg tgggtgaaga gaagatgtct ctaagaaacc ggctgtcaaa
1441 gtccagggaa aatcctgagg aagatgaaga ccagagaaac cctgcaaagg agtccctaga
1501 gacacctagt aatggtcgaa ttgacataaa acagttgata gcaaagaaga taaagttgac
1561 agcagaggca gaggaattga agccattttt tatgaaggaa gttggcagtc actttgatga
1621 ttttgtgacc aatctcattg aaaagtcagc atcattagat aatggtgggt gcgctctcac
1681 aaccttttct gttcttgaag gagagaaaaa caaccataga gcgaaggatt tgagagcacc
1741 tccagaacaa ggaaagattt ttattgcaag gcgctctctc ttagatgaac tgcttgaagt
1801 ggaccacatc agaacaatat atcacatgtt tattgccctc ctcattctct ttatcctcag
1861 cacacttgta gtagattaca ttgatgaagg aaggctggtg cttgagttca gcctcctgtc
1921 ttatgctttt ggcaaatttc ctaccgttgt ttggacctgg tggatcatgt tcctgtctac
1981 attttcagtt ccctattttc tgtttcaaca ttgggccact ggctatagca agagttctca
2041 tccgctgatc cgttctctct tccatggctt tcttttcatg atcttccaga ttggagttct
2101 aggttttgga ccaacatatg ttgtgttagc atatacactg ccaccagctt cccggttcat
2161 cattatattc gagcagattc gttttgtaat gaaggcccac tcatttgtca gagagaacgt
2221 gcctcgggta ctaaattcag ctaaggagaa atcaagcact gttccaatac ctacagtcaa
2281 ccagtatttg tacttcttat ttgctcctac ccttatctac cgtgacagct atcccaggaa
2341 tcccactgta agatggggtt atgtcgctat gaagtttgca caggtctttg gttgcttttt
2401 ctatgtgtac tacatctttg aaaggctttg tgcccccttg tttcggaata tcaaacagga
2461 gcccttcagc gctcgtgttc tggtcctatg tgtatttaac tccatcttgc caggtgtgct
2521 gattctcttc cttacttttt ttgccttttt gcactgctgg ctcaatgcct ttgctgagat
2581 gttacgcttt ggtgacagga tgttctataa ggattggtgg aactccacgt catactccaa
2641 ctattataga acctggaatg tggtggtcca tgactggcta tattactatg cttacaagga
2701 ctttctctgg tttttctcca agagattcaa atctgctgcc atgttagctg tctttgctgt
2761 atctgctgta gtacacgaat atgccttggc tgtttgcttg agctttttct atcccgtgct
2821 cttcgtgctc ttcatgttct ttggaatggc tttcaacttc attgtcaatg atagtcggaa
2881 aaagccgatt tggaatgttc tgatgtggac ttctcttttc ttgggcaatg gagtcttact
2941 ctgcttttat tctcaagaat ggtatgcacg tcggcactgt cctctgaaaa atcccacatt
3001 tttggattat gtccggccac gttcctggac ttgtcgttac gtgttttaga agcttggact
3061 ttgtttcctc cttgtcactg aagattgggt agctccctga tttggagcca gctgtttcca
3121 gttgttactg aagttatctg tgttatttgg accactccag gctttacaga tgactcactc
3181 cattcctagg tcacttgaag ccaaactgtt ggaagttcac tggagtcttg tacacttaag
3241 cagagcagaa ctttttttgt ggggctgggt ggggggagaa gaccgactaa cagctgaagt
3301 aatgacagat tgttgctggg tcatatcagc tttatccctt ggtaattata tctgttttgt
3361 ttcttgactc tgtccaatca gagaataaac atcatagttt cttggccact gaattagcca
3421 aaacacttag gaagaaatca cttaaatacc tctggcttag aaattttttc atgcacactg
3481 ttggaatgta tgctaattga acatgcaatt ggggaagaaa aaatgtagaa tgatttttgc
3541 tatttctagt agaaagaaaa tgtctgtttt ccaaagataa tgttatacat cctattttgt
3601 aatttttttg aaaaaagttc aatgttcagt tttccttagt ttttaccttg ttttctctat
3661 aggtcatgat ttctgtgaag caaaaagatg ccttttacca tgaattcttg agtttacatc
3721 aataatattg tatattaagg ggatcagaag taggaaggaa aaaataagag atagcagagg
3781 aaaaagaaaa acatttcctc ttataacttc tgaagtaatt tgtaaaaaag atttgtagag
3841 tcaatcatgt gtttaaatta ttttatcaca aacttaacat ggaagatatt cctttttaac
3901 tttgtggtaa cttctttgaa gttatttaga aatatccttt ggaacaatta ttttattgtc
3961 taataaatat tgacttctct tgaattattt tgcagactag tgagtctgta c
//