LOCUS HUMPCP 2060 bp mRNA linear HUM 14-MAY-1996
DEFINITION Human prolylcarboxypeptidase mRNA, complete cds.
ACCESSION L13977
VERSION L13977.1
KEYWORDS prolylcarboxypeptidase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2060)
AUTHORS Yang,H.Y., Erdos,E.G. and Chiang,T.S.
TITLE New enzymatic route for the inactivation of angiotensin
JOURNAL Nature 218 (148), 1224-1226 (1968)
PUBMED 4297812
REFERENCE 2 (bases 1 to 2060)
AUTHORS Odya,C.E., Marinkovic,D.V., Hammon,K.J., Stewart,T.A. and
Erdos,E.G.
TITLE Purification and properties of prolylcarboxypeptidase
(angiotensinase C) from human kidney
JOURNAL J. Biol. Chem. 253 (17), 5927-5931 (1978)
PUBMED 28321
REFERENCE 3 (bases 1 to 2060)
AUTHORS Tan,F., Morris,P.W., Skidgel,R.A. and Erdos,E.G.
TITLE Sequencing and cloning of human prolylcarboxypeptidase
(angiotensinase C). Similarity to both serine carboxypeptidase and
prolylendopeptidase families
JOURNAL J. Biol. Chem. 268 (22), 16631-16638 (1993)
PUBMED 8344943
COMMENT Original source text: Homo sapiens (tissue library: lambda gt10 of
Graeme I. Bell) kidney cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..2060
/db_xref="H-InvDB:HIT000192236"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="kidney"
/germline
/tissue_lib="lambda gt10 of Graeme I. Bell"
5'UTR 1..29
/note="putative"
/citation=[3]
CDS 30..1520
/EC_number="3.4.16.2"
/function="cleaves C-terminal amino acids linked to
penultimate proline"
/standard_name="angiotensinase C"
/experiment="experimental evidence, no additional details
recorded"
/note="Human prolylcarboxypeptidase is a lysosomal enzyme
with an acid pH optimum that cleaves C-terminal amino
acids linked to proline in peptides such as angiotensin II
and des-Arg9-bradykinin."
/citation=[2]
/citation=[3]
/codon_start=1
/product="prolylcarboxypeptidase"
/protein_id="AAA99891.1"
/translation="MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAV
AKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNN
TGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKH
LKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVF
MKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLK
DWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVY
YNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSD
DCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTI
SEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH"
sig_peptide 30..119
/note="putative"
/citation=[3]
mat_peptide 165..1517
/product="prolylcarboxypeptidase"
/EC_number="3.4.16.2"
/function="cleaves C-terminal amino acids linked to
penultimate proline"
/experiment="experimental evidence, no additional details
recorded"
/citation=[2]
/citation=[3]
3'UTR 1518..2060
/note="putative"
/citation=[3]
regulatory 2038..2043
/regulatory_class="polyA_signal_sequence"
/note="putative"
/citation=[3]
polyA_site 2060
/note="truncated 70 bp polyA tail; putative"
/citation=[3]
BASE COUNT 534 a 462 c 462 g 602 t
ORIGIN
1 cacccgcact gcagtctcca gcctgagcca tgggccgccg agccctcctg ctcctgcttc
61 tgtcttttct ggcgccctgg gccaccatag ccctccggcc ggccttaagg gccctcggca
121 gcctacactt gccaaccaac cccacatccc tcccggctgt agccaagaac tattcggttc
181 tctacttcca acagaaggtt gatcattttg gatttaatac tgtgaaaact tttaatcagc
241 ggtacctagt agctgataaa tactggaaga aaaatggtgg atcaatactt ttctacactg
301 gtaatgaagg ggacattatc tggttttgta ataacacggg gttcatgtgg gatgtggctg
361 aggaactgaa agctatgttg gtgtttgctg aacatcgata ctatggagag tctctcccct
421 ttggtgacaa ctcattcaag gattccagac acttgaattt cctgacatca gaacaagctc
481 tggctgattt tgcagagtta atcaaacact tgaaaagaac aatcccagga gctgaaaatc
541 aacctgtcat tgccatagga ggctcctatg gtggcatgct tgccgcctgg tttaggatga
601 aatatcctca tatggtagtt ggagctcttg cagcttctgc ccctatctgg cagtttgagg
661 atttagtacc ttgtggtgta tttatgaaga tcgtaactac agattttagg aaaagcggtc
721 cacattgttc agagagcatc cacaggtcct gggatgccat taatcgactc tcaaatactg
781 gcagtggttt gcagtggctt actggagccc ttcacttatg cagcccatta acttctcagg
841 acatccaaca tttgaaagac tggatctctg aaacctgggt gaatctggca atggtggact
901 atccttatgc ctctaacttt ttacagcctt tgcctgcttg gcctatcaag gtagtgtgcc
961 agtatttgaa aaatcccaac gtatctgatt cactgctgct gcagaatatt ttccaagctc
1021 tgaatgtata ttacaattat tcgggccagg tgaaatgcct gaatatttca gagacagcaa
1081 ctagcagtct gggaacactg ggttggagct atcaggcctg cacagaagta gtcatgccct
1141 tttgtactaa tggtgtcgat gacatgtttg aacctcactc atggaactta aaggaacttt
1201 ctgatgactg ttttcaacag tggggtgtga gaccaaggcc ctcctggatc actactatgt
1261 atggaggcaa aaacattagt tcacacacaa acattgtttt cagcaatggt gaactagacc
1321 cctggtcagg aggtggagta actaaggata tcacagacac tctggttgca gtcaccatct
1381 cagagggggc ccaccactta gatctccgca ccaagaatgc cttggatcct atgtctgtgc
1441 tgttagcccg ctccttggaa gttagacata tgaagaattg gatcagagat ttctatgaca
1501 gtgcaggaaa gcagcactga gaaacttttg attgttttca atttcttctt ttatgttcac
1561 accaccacat tcccattcac tttgattttc tacatgtaat taccttcttt tgtttatcat
1621 tagatttgat ggggccaaag ttgagataga atagagggtg atgacggtaa gagcaagtgt
1681 cccatgaatg tgatttcctg ggttctcact gtctttgcac cacgtctagg aagaatcttc
1741 ttgatagctc tcccacacca tcagtggccc tcataactgg agtagagttc ctggttgctt
1801 ttcataagag ggagagttac tttctttgta tctctgcaag cagagatttc tctttggttt
1861 tgaggttgaa gtgtctttgg cccatttgta agtccccatc cctaccctac acaaagtaaa
1921 agcagaagat agataaaaaa tgatgtaatt gcagctggta ggatgtctgg tgcccaatcc
1981 caggaagtga gagccatttc ttttgtactg gatttaatga ctttgaactg tgctgtaaat
2041 aaataataca gctggacctt
//