LOCUS HUMHGBE 2955 bp mRNA linear HUM 31-DEC-1994
DEFINITION Homo sapiens 1,4-alpha-glucan branching enzyme (HGBE) mRNA,
complete cds.
ACCESSION L07956
VERSION L07956.1
KEYWORDS 1,4-alpha-glucan branching enzyme.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2955)
AUTHORS Thon,V.J., Khalil,M. and Cannon,J.F.
TITLE Isolation of human glycogen branching enzyme cDNAs by screening
complementation in yeast
JOURNAL J. Biol. Chem. 268 (10), 7509-7513 (1993)
PUBMED 8463281
COMMENT Original source text: Homo sapiens liver cDNA to mRNA.
FEATURES Location/Qualifiers
source 1..2955
/db_xref="H-InvDB:HIT000191877"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_type="hepatocyte"
/tissue_type="liver"
gene 1..2955
/gene="HGBE"
misc_feature 1..12
/gene="HGBE"
/experiment="experimental evidence, no additional details
recorded"
/note="this is the 5' adaptor sequence used to clone cDNAs
in this library"
CDS 91..2199
/gene="HGBE"
/EC_number="2.4.1.18"
/function="branch glycogen"
/standard_name="glycogen branching enzyme"
/experiment="experimental evidence, no additional details
recorded"
/codon_start=1
/product="1,4-alpha-glucan branching enzyme"
/protein_id="AAA58642.1"
/translation="MAAPMTPAARPEDYEAALNAALADVPELARLLEIDPYLKPYAVD
FQRRYKQFSQILKNIGENEGGIDKFSRGYESFGVHRCADGGLYSKEWAPGAEGVFLTG
DFNGWNPFSYPYKKLDYGKWELYIPPKQNKSVLVPHGSKLKVVITSKSGEILYRISPW
AKYVVREGDNVNYDWIHWDPEHSYEFKHSRPKKPRSLRIYESHVGISSHEGKVASYKH
FTCNVLPRIKGLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGTPEELQELVDTA
HSMGIIVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRGTHDLWDSRLFAYSSWEV
LRFLLSNIRWWLEEYRFDGFRFDGVTSMLYHHHGVGQGFSGDYSEYFGLQVDEDALTY
LMLANHLVHTLCPDSITIAEDVSGMPALCSPISQGGGGFDYRLAMAIPDKWIQLLKEF
KDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLAFWLMDAEMYTNMSVLTP
FTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQ
FHLTDDDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEKHEGNKIIAFERAGLLFI
FNFHPSKSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDHSTDFFSEAFEHNGRPYS
LLVYIPSRVALILQNVDLPN"
misc_feature 2900..2955
/gene="HGBE"
/experiment="experimental evidence, no additional details
recorded"
/note="this is the 3' adaptor used in the cDNA library
construction; the 16 a's should be considered the true 3'
end, although in vivo length may actually be longer"
BASE COUNT 853 a 562 c 650 g 890 t
ORIGIN
1 gatctgaatt cggtcccagc tagagctcca gcgcccgctc aggccccact cgaccctctc
61 gggcctcggc tacttggact gcggcggaat atggcggctc cgatgactcc cgcggctcgg
121 cccgaggact acgaggcggc gctcaatgcc gccctggctg acgtgcccga actggccaga
181 ctcctggaga tcgacccgta cttgaagccc tacgccgtgg acttccagcg caggtataag
241 cagtttagcc aaattttgaa gaacattgga gaaaatgaag gtggtattga taagttttcc
301 agaggctatg aatcatttgg cgtccacaga tgtgctgatg gtggtttata ctccaaagaa
361 tgggccccgg gagcagaagg agtttttctt actggagatt ttaatggttg gaatccattt
421 tcgtacccat acaaaaaact ggattatgga aaatgggagc tgtatatccc accaaagcag
481 aataaatctg tactcgtgcc tcatggatcc aaattaaagg tagttattac tagtaaaagc
541 ggagagatct tgtatcgtat ttcaccgtgg gcaaagtatg tggttcgtga aggtgataat
601 gtgaattatg attggataca ctgggatcca gaacactcat atgagtttaa gcattccaga
661 ccaaagaagc cacggagtct aagaatttat gaatctcatg tgggaatttc ttcccatgaa
721 ggaaaagtag cttcttataa acattttaca tgcaatgtac taccaagaat caaaggcctt
781 ggatacaact gcattcagtt gatggcaatc atggagcatg cttactatgc cagctttggt
841 taccaaatca caagcttctt tgcagcttcc agccgttatg gaacacctga agagctacaa
901 gaactggtag acacagctca ttccatgggt atcatagtcc tcttagatgt ggtacacagc
961 catgcttcaa aaaattcagc agatggattg aatatgtttg atgggacaga ttcctgttat
1021 tttcattctg gacctagagg gactcatgat ctttgggata gcagattgtt tgcctactcc
1081 agctgggaag ttttaagatt ccttctgtca aacataagat ggtggttgga agaatatcgc
1141 tttgatggat ttcgttttga tggtgttacg tccatgcttt atcatcacca tggagtgggt
1201 caaggtttct caggtgatta cagtgaatat ttcggactac aagtagatga agatgccttg
1261 acttacctca tgttggcaaa tcatttggtt cacacgctgt gtcccgattc tataacaata
1321 gctgaggatg tatcaggaat gccagctctg tgctctccaa tttcccaggg agggggtggt
1381 tttgactatc gactagccat ggcaattcca gataagtgga ttcagctact taaagagttt
1441 aaagatgaag actggaacat gggcgatata gtatacacgc tcacaaacag gcgctacctt
1501 gaaaagtgca ttgcttatgc agagagccat gatcaggcat tggttgggga taagtcgctg
1561 gcattttggt tgatggatgc cgaaatgtat acaaacatga gtgtcctgac tccttttact
1621 ccagttattg atcgtggaat acagcttcat aaaatgattc gactcattac gcatgggctt
1681 ggtggagaag gctatctcaa tttcatgggt aatgaatttg ggcatcctga atggttagac
1741 ttcccaagaa aaggaaataa tgagagttac cattatgcca ggcggcagtt tcatttaact
1801 gacgacgacc ttcttcgcta caagttccta aataattttg acagggatat gaatagattg
1861 gaagaaagat atggttggct tgcagctcca caggcctacg tgagtgaaaa acatgaaggc
1921 aataagatca ttgcttttga aagagcaggt cttcttttca ttttcaactt ccatccaagc
1981 aagagctaca ctgactaccg agttggaaca gcattgccag ggaaattcaa aattgtgcta
2041 gattcagatg cagcggaata tggagggcat cagagactgg accacagcac tgactttttt
2101 tctgaggctt ttgaacataa tgggcgtccc tattctcttt tggtgtacat tccaagcaga
2161 gtggccctca tccttcagaa tgtggatctg ccgaattgaa gaggcctgat ttcagctcca
2221 ccagatgcag atttgtgttt tgttttcttg ttatcactgt cacacagctt ataacatgta
2281 tgcttttcag aatacagttg tctagccaag ccatcaagtg tctgaaattc aatattggtt
2341 tatgcaaata cagcaaactt ttatttaagt agataggaga atatgtttaa aatattagga
2401 atcctagacc atattttcaa gtcatcttag cagctaggat tctcaaatgg aagtgttata
2461 tataatatgt taaaaacatt ttgctttcct ggctaattat ttgatccttt taaatccaaa
2521 tttgaatcat ttgtcatgta tgattatttc tgttaaatgt acacagtatt taagatggat
2581 atttggtggc tctatttgtt ctgatatctt ttggtctaaa ttatgaggta ccaagattgt
2641 ttctttgttt ctttttttca aattgtgttt agaaatactg taataaatat gcagtagtga
2701 tataaagaat tatatccaag gtaatataaa agccattacg tatgaactca tccgtgtctc
2761 attttgtgtt ttattttgtg atctcttgtc cactaagtat cttgttaaat gccagtatct
2821 cagtctttct gaagccctga aatggtaatt gtagcatttc agaaaatgtc tttcatttca
2881 atcaataaaa agcttttgta aaaaaaaaaa aaaaaaaaaa aaaaaccgtc gacaaagcgg
2941 ccgcaaaccg aattc
//