LOCUS KF032815 1807 bp mRNA linear HUM 22-MAY-2013
DEFINITION Homo sapiens EPHX1 (EPHX1) mRNA, complete cds.
ACCESSION KF032815
VERSION KF032815.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1807)
AUTHORS Nguyen,H.L., Yang,X. and Omiecinski,C.J.
TITLE Expression of a novel mRNA transcript for human microsomal epoxide
hydrolase (EPHX1) is regulated by short open reading frames within
its 5'-untranslated region
JOURNAL RNA 19 (6), 752-766 (2013)
PUBMED 23564882
REFERENCE 2 (bases 1 to 1807)
AUTHORS Nguyen,H.L.T., Yang,X. and Omiecinski,C.J.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2013) Veterinary and Biomedical Sciences, The
Pennsylvania State University, 101 Life Sciences Building,
University Park, PA 16802, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..1807
/db_xref="H-InvDB:HIT000722612"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="HepG2"
/tissue_type="liver"
/PCR_primers="fwd_name: ephx1 fp, fwd_seq:
tggcaaagtttcctctttgtcccg, rev_name: ephx1 rp, rev_seq:
atggaggcctggaaaggaagttct"
/note="identical sequence found in cell line A549"
gene 1..1807
/gene="EPHX1"
CDS 210..1577
/gene="EPHX1"
/codon_start=1
/product="EPHX1"
/protein_id="AGL94507.1"
/translation="MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAARE
DDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFD
WKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKI
IPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQ
EFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTE
RDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTN
TEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKV
YVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLS
VLERQ"
BASE COUNT 401 a 542 c 467 g 397 t
ORIGIN
1 ggaattccgc gtcccagact gtgggcggac gccgcaggga gggaggctcg cgaccccgag
61 agcgcaccgc gtcccctggc ctgggcgagc tctgcttcca gggccgtggc tcgctcggcg
121 gtgaaatgca cttaatttgt cacccaagtc ggaacactga ttttcatgcg cgtccgtgtg
181 aagagaccac caaacaggct ttgtgagcca tgtggctaga aatcctcctc acttcagtgc
241 tgggctttgc catctactgg ttcatctccc gggacaaaga ggaaactttg ccacttgaag
301 atgggtggtg ggggccaggc acgaggtccg cagccaggga ggacgacagc atccgccctt
361 tcaaggtgga aacgtcagat gaggagatcc acgacttaca ccagaggatc gataagttcc
421 gtttcacccc acctttggag gacagctgct tccactatgg cttcaactcc aactacctga
481 agaaagtcat ctcctactgg cggaatgaat ttgactggaa gaagcaggtg gagattctca
541 acagataccc tcacttcaag actaagattg aagggctgga catccacttc atccacgtga
601 agccccccca gctgcccgca ggccataccc cgaagccctt gctgatggtg cacggctggc
661 ccggctcttt ctacgagttt tataagatca tcccactcct gactgacccc aagaaccatg
721 gcctgagcga tgagcacgtt tttgaagtca tctgcccttc catccctggc tatggcttct
781 cagaggcatc ctccaagaag gggttcaact cggtggccac cgccaggatc ttttacaagc
841 tgatgctgcg gctgggcttc caggaattct acattcaagg aggggactgg gggtccctga
901 tctgcactaa tatggcccag ctggtgccca gccacgtgaa aggcctgcac ttgaacatgg
961 ctttggtttt aagcaacttc tctaccctga ccctcctcct gggacagcgt ttcgggaggt
1021 ttcttggcct cactgagagg gatgtggagc tgctgtaccc cgtcaaggag aaggtattct
1081 acagcctgat gagggagagc ggctacatgc acatccagtg caccaagcct gacaccgtag
1141 gctctgctct gaatgactct cctgtgggtc tggctgccta tattctagag aagttttcca
1201 cctggaccaa tacggaattc cgatacctgg aggatggagg cctggaaagg aagttctccc
1261 tggacgacct gctgaccaac gtcatgctct actggacaac aggcaccatc atctcctccc
1321 agcgcttcta caaggagaac ctgggacagg gctggatgac ccagaagcat gagcggatga
1381 aggtctatgt gcccactggc ttctctgcct tcccttttga gctattgcac acgcctgaaa
1441 agtgggtgag gttcaagtac ccaaagctca tctcctattc ctacatggtt cgtgggggcc
1501 actttgcggc ctttgaggag ccggagctgc tcgcccagga catccgcaag ttcctgtcgg
1561 tgctggagcg gcaatgaccc acccctctcc ccccgcctgc cacctccccc cacaagtgcc
1621 ctccaggctt ttcttgggga agatacccct tttctgagga atgagtttgc ctccgtcccc
1681 tgcccatgct gggagcccac gctcaccccc tcacccctcc aagctcactc cccaaccccc
1741 aactccgtgt ggtaagcaac atggctttga tgataaacga ctttactcta aaaaaaaaaa
1801 aaaaaaa
//