LOCUS HUMOTC 1464 bp mRNA linear HUM 07-JAN-1995
DEFINITION Human ornithine transcarbamylase (OTC) mRNA, complete coding
sequence.
ACCESSION K02100
VERSION K02100.1
KEYWORDS nuclear matrix protein; ornithine transcarbamylase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1464)
AUTHORS Horwich,A.L., Fenton,W.A., Williams,K.R., Kalousek,F., Kraus,J.P.,
Doolittle,R.F., Konigsberg,W. and Rosenberg,L.E.
TITLE Structure and expression of a complementary DNA for the nuclear
coded precursor of human mitochondrial ornithine transcarbamylase
JOURNAL Science 224 (4653), 1068-1074 (1984)
PUBMED 6372096
COMMENT Original source text: Human cDNA to adult liver mRNA, clones pHO-1,
pHO-7, and pHO-31.
The structural gene for ornithine transcarbamylase (OTC) is a
nuclear-coded polypeptide encoded on the X chromosome. OTC
consists of 3 identical subunits. The precursor for each subunit
is synthesized in the cytoplasm and then processed
posttranslationally to its mature form during mitochondrial import.
Comparison of the human mature OTC subunit sequence with that of
the rat revealed 90% amino acid homology, and 95% DNA sequence
homology.
The cDNA sequence of OTC was derived from three overlapping
sequences from plasmids pHO-1, pHO-7, and pHO-31.
FEATURES Location/Qualifiers
source 1..1464
/db_xref="H-InvDB:HIT000191380"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="Xp21.1"
/tissue_type="liver"
/dev_stage="adult"
gene 1..1464
/gene="OTC"
mRNA <1..>1464
/gene="OTC"
/product="OTC mRNA"
CDS 136..1200
/gene="OTC"
/codon_start=1
/product="ornithine transcarbamylase"
/protein_id="AAA59975.1"
/db_xref="GDB:G00-119-468"
/translation="MLFNLRILLNNAAFRNGHNFMVRNFRCGQPLQNKVQLKGRDLLT
LKNFTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFA
LLGGHPCFPTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEASIPII
NGLSDLYHPIQILADYLTLQEHYSSLKGLTLSCFGDGNNILHSIMMSAAKFGMHLQAA
TPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGREEEKKK
RLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTI
MAVMVSLLTDYSPQLQKPKF"
sig_peptide 136..231
/gene="OTC"
/note="G00-119-468"
mat_peptide 232..1197
/gene="OTC"
/product="ornithine transcarbamylase"
/note="G00-119-468"
BASE COUNT 445 a 297 c 323 g 399 t
ORIGIN 243 bp upstream of PvuII site on X chromosome.
1 aagctgaagg gtgatattac ctttgctccc tcactgcaac tgaacacatt tcttagtttt
61 taggtggccc ccgctggcta acttgctgtg gagttttcaa gggcatagaa tcgtccttta
121 cacaattaaa agaagatgct gtttaatctg aggatcctgt taaacaatgc agcttttaga
181 aatggtcaca acttcatggt tcgaaatttt cggtgtggac aaccactaca aaataaagtg
241 cagctgaagg gccgtgacct tctcactcta aaaaacttta ccggagaaga aattaaatat
301 atgctatggc tatcagcaga tctgaaattt aggataaaac agaaaggaga gtatttgcct
361 ttattgcagg ggaagtcctt aggcatgatt tttgagaaaa gaagtactcg aacaagattg
421 tctacagaaa caggctttgc acttctggga ggacatcctt gttttcctac cacacaagat
481 attcatttgg gtgtgaatga aagtctcacg gacacggccc gtgtattgtc tagcatggca
541 gatgcagtat tggctcgagt gtataaacaa tcagatttgg acacccttgc taaagaagca
601 tccatcccaa ttatcaatgg gctgtcagat ttgtaccatc ctatccagat cctggctgat
661 tacctcacgc tccaggaaca ctatagctct ctgaaaggtc ttaccctcag ctgtttcggg
721 gatgggaaca atatcctgca ctccatcatg atgagcgcag cgaaattcgg aatgcacctt
781 caggcagcta ctccaaaggg ttatgagccg gatgctagtg taaccaagtt ggcagagcag
841 tatgccaaag agaatggtac caagctgttg ctgacaaatg atccattgga agcagcgcat
901 ggaggcaatg tattaattac agacacttgg ataagcatgg gacgagaaga ggagaagaaa
961 aagcggctcc aagctttcca aggttaccaa gttacaatga agactgctaa agttgctgcc
1021 tctgactgga catttttaca ctgcttgccc agaaagccag aagaagtgga tgatgaagtc
1081 ttttattctc ctcgatcact agtgttccca gaggcagaaa acagaaagtg gacaatcatg
1141 gctgtcatgg tgtccctgct gacagattac tcacctcagc tccagaagcc taaattttga
1201 tgttgtgtta cttgtcaaga aagaagcaat gttggtcagt aacagaatga gttggtttat
1261 ggggaaaaga gaagagaatc taaaaaataa accaatccct aacacgtggt atgggcgaat
1321 cgtacgatat gctttgccat tgtgaaactt tccttaagcc ttcaatttaa gtgctgatgc
1381 actgtaatac gtgcttaact ttgcttaaac tctctaattc ccaatttctg agttacattt
1441 agatatcata ttaactatca tata
//