LOCUS JQ425695 1381 bp mRNA linear HUM 12-MAY-2012
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
splice variant 2 (PFKFB1) mRNA, complete cds, alternatively
spliced.
ACCESSION JQ425695
VERSION JQ425695.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1381)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE Unique alternative splice variants of PFKFB1
JOURNAL Adv Biol Chem (2012) In press
REFERENCE 2 (bases 1 to 1381)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE The biological significance of alternative splice variants of PFKFB
JOURNAL Mol. Cell. Biochem. (2012) In press
REFERENCE 3 (bases 1 to 1381)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE Direct Submission
JOURNAL Submitted (20-JAN-2012) Molecular Biology, Palladin Institute of
Biochemistry, 9 Leontovycha, Kiev 01601, Ukraine
FEATURES Location/Qualifiers
source 1..1381
/db_xref="H-InvDB:HIT000721395"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="X"
/map="Xp11.21"
/cell_line="U87"
/cell_type="glioma"
/PCR_primers="fwd_name: pfkfb1f, fwd_seq:
ggtgcgactcctaagatgtc, rev_name: pfkfb1r, rev_seq:
ctgagccctttccaagaagt"
gene 1..1381
/gene="PFKFB1"
CDS 16..1365
/gene="PFKFB1"
/EC_number="3.1.3.46"
/note="lacks part of exon 3; product of this splice
variant lacks the 6-phosphofructo-2-kinase activity of
bifunctional enzyme, but does retain
fructose-2,6-biphosphatase activity; alternatively
spliced"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 1 splice variant 2"
/protein_id="AFJ19240.1"
/translation="MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTM
VIMVGLPARGKTYISTKLTRYLNWIGTPTKDNMEALQIRKQCALAALKDVHNYLSHEE
GHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYI
DCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRT
VYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISS
LKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQD
KYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPY
LKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY"
BASE COUNT 380 a 361 c 348 g 292 t
ORIGIN
1 ggtgcgactc ctaagatgtc tccagagatg ggagagctca cccaaaccag gttgcagaag
61 atctggattc cacacagcag cggcagcagc aggctgcaac ggagaagggg ctcatccata
121 ccccagttta ccaattcccc cacaatggtg atcatggtgg gtttaccagc tcgaggcaag
181 acctatatct ccacaaagct cacacgatat ctcaactgga taggaacacc aactaaagac
241 aacatggaag ccctgcaaat caggaagcag tgcgccctgg cagccctgaa ggatgttcac
301 aactatctca gccatgagga aggtcatgtt gcggtttttg atgccaccaa cactaccaga
361 gaacgacggt cactgatcct gcagtttgca aaagaacatg gttacaaggt gtttttcatt
421 gagtccattt gtaatgaccc tggcataatt gcagaaaaca tcaggcaagt gaaacttggc
481 agccctgatt atatagactg tgaccgggaa aaggttctgg aagactttct aaagagaatt
541 gagtgctatg aggtcaacta ccaacccttg gatgaggaac tggacagcca cctgtcctac
601 atcaagatct tcgacgtggg cacacgctac atggtgaacc gagtgcagga tcacatccag
661 agccgcacag tctactacct catgaatatc catgtcacac ctcgctccat ctacctttgc
721 cgacatggcg agagtgaact caacatcaga ggccgcatcg gaggtgactc tggcctctca
781 gttcgcggca agcagtatgc ctatgccctg gccaacttca ttcagtccca gggcatcagc
841 tccctgaagg tgtggaccag tcacatgaag aggaccatcc agacagctga ggccctgggt
901 gtcccctatg agcagtggaa ggccctgaat gagattgatg cgggtgtctg tgaggagatg
961 acctatgaag aaatccagga acattaccct gaagaatttg cactgcgaga ccaagataaa
1021 tatcgctacc gctatcccaa gggagagtcc tatgaggatc tggttcagcg tctggagcca
1081 gtgataatgg agctagaacg acaggagaat gtactggtga tctgccacca ggctgtcatg
1141 cggtgcctcc tggcctattt cctggataaa agttcagatg agcttccata tctcaagtgc
1201 cctctgcaca cagtgctcaa actcactcct gtggcttatg gctgcaaagt ggaatccatc
1261 tacctgaatg tggaggccgt gaacacacac cgggagaagc ctgagaatgt ggacatcacc
1321 cgggaacctg aggaagccct ggatactgtc ccagcccact actgagccct ttccaagaag
1381 t
//