LOCUS JQ425694 1353 bp mRNA linear HUM 12-MAY-2012
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
splice variant 3 (PFKFB1) mRNA, complete cds, alternatively
spliced.
ACCESSION JQ425694
VERSION JQ425694.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1353)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE Unique alternative splice variants of PFKFB1
JOURNAL Adv Biol Chem (2012) In press
REFERENCE 2 (bases 1 to 1353)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE The biological significance of alternative splice variants of PFKFB
JOURNAL Mol. Cell. Biochem. (2012) In press
REFERENCE 3 (bases 1 to 1353)
AUTHORS Minchenko,D.O., Lypova,N.M., Moenner,M. and Minchenko,O.H.
TITLE Direct Submission
JOURNAL Submitted (20-JAN-2012) Molecular Biology, Palladin Institute of
Biochemistry, 9 Leontovycha, Kiev 01601, Ukraine
FEATURES Location/Qualifiers
source 1..1353
/db_xref="H-InvDB:HIT000721394"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="X"
/map="Xp11.21"
/cell_line="U87"
/cell_type="glioma"
/PCR_primers="fwd_name: pfkfb1f, fwd_seq:
ggtgcgactcctaagatgtc, rev_name: pfkfb1r, rev_seq:
ctgagccctttccaagaagt"
gene 1..1353
/gene="PFKFB1"
CDS 603..1337
/gene="PFKFB1"
/EC_number="3.1.3.46"
/note="lacks exon 3; product of this splice variant could
not catalyze the synthesis of fructose-2,6-biphosphate,
but does catalyze degradation of fructose-2,6-biphosphate;
alternatively spliced"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 1 splice variant 3"
/protein_id="AFJ19239.1"
/translation="MVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIG
GDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEI
DAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQEN
VLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVN
THREKPENVDITREPEEALDTVPAHY"
BASE COUNT 368 a 354 c 342 g 289 t
ORIGIN
1 ggtgcgactc ctaagatgtc tccagagatg ggagagctca cccaaaccag gttgcagaag
61 atctggattc cacacagcag cggcagcagc aggctgcaac ggagaagggg ctcatccata
121 ccccagttta ccaattcccc cacaatggtg atcatggtgg gtttaccagc tcgaggcaag
181 acctatatct ccacaaagct cacacgatat ctcaactgga taggaacacc aactaaaggc
241 agtgcgccct ggcagccctg aaggatgttc acaactatct cagccatgag gaaggtcatg
301 ttgcggtttt tgatgccacc aacactacca gagaacgacg gtcactgatc ctgcagtttg
361 caaaagaaca tggttacaag gtgtttttca ttgagtccat ttgtaatgac cctggcataa
421 ttgcagaaaa catcaggcaa gtgaaacttg gcagccctga ttatatagac tgtgaccggg
481 aaaaggttct ggaagacttt ctaaagagaa ttgagtgcta tgaggtcaac taccaaccct
541 tggatgagga actggacagc cacctgtcct acatcaagat cttcgacgtg ggcacacgct
601 acatggtgaa ccgagtgcag gatcacatcc agagccgcac agtctactac ctcatgaata
661 tccatgtcac acctcgctcc atctaccttt gccgacatgg cgagagtgaa ctcaacatca
721 gaggccgcat cggaggtgac tctggcctct cagttcgcgg caagcagtat gcctatgccc
781 tggccaactt cattcagtcc cagggcatca gctccctgaa ggtgtggacc agtcacatga
841 agaggaccat ccagacagct gaggccctgg gtgtccccta tgagcagtgg aaggccctga
901 atgagattga tgcgggtgtc tgtgaggaga tgacctatga agaaatccag gaacattacc
961 ctgaagaatt tgcactgcga gaccaagata aatatcgcta ccgctatccc aagggagagt
1021 cctatgagga tctggttcag cgtctggagc cagtgataat ggagctagaa cgacaggaga
1081 atgtactggt gatctgccac caggctgtca tgcggtgcct cctggcctat ttcctggata
1141 aaagttcaga tgagcttcca tatctcaagt gccctctgca cacagtgctc aaactcactc
1201 ctgtggctta tggctgcaaa gtggaatcca tctacctgaa tgtggaggcc gtgaacacac
1261 accgggagaa gcctgagaat gtggacatca cccgggaacc tgaggaagcc ctggatactg
1321 tcccagccca ctactgagcc ctttccaaga agt
//