LOCUS       JQ346088                 159 bp    mRNA    linear   HUM 11-MAR-2014
DEFINITION  Homo sapiens epidermal growth factor precursor (EGF) mRNA, partial
            cds.
ACCESSION   JQ346088
VERSION     JQ346088.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 159)
  AUTHORS   Khan,M.A., Khan,F., Ahmad,N., Khan,M.I., Zafar,A.U. and Husnain,T.
  TITLE     Expression line approach to recombinant human epidermal growth
            factor into the yeast, Pichia pastoris from Huh-7 cell line
  JOURNAL   Mol. Biol. Rep. 41 (3), 1445-1451 (2014)
   PUBMED   24413989
REFERENCE   2  (bases 1 to 159)
  AUTHORS   Khan,M.A., Khan,F. and Khan,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2012) Biopharmaceutical Lab, CEMB, University of
            the Punjab, 87-West Canal Canal Road, Lahore, Punjab 54000,
            Pakistan
FEATURES             Location/Qualifiers
     source          1..159
                     /db_xref="H-InvDB:HIT000721382"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q25-q27"
                     /cell_line="Huh-7"
                     /cell_type="hepato carcinoma"
                     /PCR_primers="fwd_name: se1, fwd_seq:
                     tcacctcagggaagatgacc, rev_name: se2, rev_seq:
                     atcccatcctcagtgtcagc"
                     /PCR_primers="fwd_seq: cagggaagatgaccaccact, rev_seq:
                     tgcgactcctcacatctctg"
                     /PCR_primers="fwd_seq: aagaattcaatagtgactctgaa, rev_seq:
                     gcgcggccgcgcgcagttcccacca"
     gene            <1..>159
                     /gene="EGF"
                     /note="hEGF"
     CDS             <1..>159
                     /gene="EGF"
                     /codon_start=1
                     /product="epidermal growth factor precursor"
                     /protein_id="AFA26280.1"
                     /translation="NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQY
                     RDLKWWELR"
     mat_peptide     1..159
                     /gene="EGF"
                     /product="epidermal growth factor"
BASE COUNT           36 a           33 c           48 g           42 t
ORIGIN      
        1 aatagtgact ctgaatgtcc cctgtcccac gatgggtact gcctccatga tggtgtgtgc
       61 atgtatattg aagcattgga caagtatgca tgcaactgtg ttgttggcta catcggggag
      121 cgatgtcagt accgagacct gaagtggtgg gaactgcgc
//