LOCUS HUMPCNA 6340 bp DNA linear HUM 07-JAN-1995
DEFINITION Human proliferating cell nuclear antigen (PCNA) gene, complete cds.
ACCESSION J04718
VERSION J04718.1
KEYWORDS proliferating cell nuclear antigen.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6340)
AUTHORS Travali,S., Ku,D.H., Rizzo,M.G., Ottavio,L., Baserga,R. and
Calabretta,B.
TITLE Structure of the human gene for the proliferating cell nuclear
antigen
JOURNAL J. Biol. Chem. 264 (13), 7466-7472 (1989)
PUBMED 2565339
COMMENT Original source text: Human leukocyte DNA, (library of C.Croce),
and cDNA to mRNA, clone EMBL3-S2.
Draft entry and printed copy of sequence [1] kindly provided by
D.-H.Ku, 15-MAR-1989.
FEATURES Location/Qualifiers
source 1..6340
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
/map="20pter-p12"
regulatory 748..753
/regulatory_class="other"
/note="GC box"
regulatory 1090..1095
/regulatory_class="other"
/note="GC box"
regulatory 1110..1115
/regulatory_class="other"
/note="GC box"
regulatory 1124..1129
/regulatory_class="CAAT_signal"
regulatory 1145..1150
/regulatory_class="other"
/note="GC box"
prim_transcript 1268..6191
/note="PCNA mRNA and introns"
CDS join(1427..1647,2356..2453,2550..2617,3556..3750,
5636..5759,5846..5925)
/gene="PCNA"
/note="proliferating cell nuclear antigen (PCNA)"
/codon_start=1
/protein_id="AAA60040.1"
/db_xref="GDB:G00-120-261"
/translation="MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSH
VSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALV
FEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVI
SCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKA
TPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS"
gene join(1427..1647,2356..2453,2550..2617,3556..3750,
5636..5759,5846..5924)
/gene="PCNA"
exon <1427..1647
/gene="PCNA"
/note="proliferating cell nuclear antigen (PCNA)"
/number=1
intron 1648..2355
/note="PCNA intron A"
exon 2356..2453
/gene="PCNA"
/note="proliferating cell nuclear antigen"
intron 2454..2549
/note="PCNA intron B"
exon 2550..2617
/gene="PCNA"
/note="proliferating cell nuclear antigen"
/number=3
intron 2618..3555
/note="PCNA intron C"
exon 3556..3750
/gene="PCNA"
/note="proliferating cell nuclear antigen"
/number=4
intron 3751..5635
/note="PCNA intron D"
exon 5636..5759
/gene="PCNA"
/note="proliferating cell nuclear antigen"
/number=5
intron 5760..5845
/note="PCNA intron E"
exon 5846..>5925
/note="proliferating cell nuclear antigen"
/number=6
BASE COUNT 1756 a 1280 c 1457 g 1847 t
ORIGIN 1 bp upstream of EcoRI site; chromosome 20pter-20q13.
1 gaattctgct gaccaaggta ttaaaagtaa ctaaagagaa gtggtgtgaa gaaagcaaga
61 gagaaacaac aaatcctgtc catcctgtaa caattgaaaa tttctggctg ggcgtggtgg
121 ctcaggcctg taatcccagc actttgagag gccgaggcag gtggatcacc tgaggtcagg
181 tgttcaagac cagcctggcc aacatggtga aaccccgtct ctactaaaaa aaaataataa
241 taataataca aaaattagcc gggtgtggtg gtaggcacct gtaatcccag atactcggga
301 ggctgaggca ggagactcac ttgaacctgg gaggcggagg ttgcaatgag ctgagatcgc
361 gcgactgtac tccagcctgg atgacagagc aggactccat ctcaaaaagg aaggcgggga
421 aaaggggaaa tattaaatgt gtacgctctt tgactcagct gtattacttc aaggagttga
481 tatcaccaaa attgcctaag tgctcaaagg tgtttgtagt taaacaacag gagattgata
541 aattatgtta tatacatgtg atgctatgtt ttaaagaggt actgatatga taaaaagatg
601 tacgtggcat aaaattaaat gtacttatta agtacttttc caagtgttta cggaatgagt
661 gcatttttga aaaaaaaaaa gtgtattcga acttttaaaa aagctttaaa agctttatac
721 aataacgatt gagtgattat aagagctggc gggggaatgt taagaggatg atagggagct
781 aagtttaaca gaacaattca cctctttatc ttgtgacacc tacgagcgca tcaattctgt
841 aattgaaaaa taaagtgcat atttgcagca gctgtactct cttcaggctg caaggaggct
901 tttcctcccg gtaggcttga tttgcatttc actttcactt tcgtggctgg aaactttcta
961 cccacgtagt gaggctagag gagccaccta aagctggggc ttgacgaagc cgggaccggg
1021 acccgatctc cacatatgcc cggacttctt ctgcggccgg gttcaggagt caaagaggcg
1081 gggagacctg cgcgacgctg ccccgccctg cgcccgcttc ctccaatgta tgctctaggg
1141 ggcgggcctc gcggggagca tggacacgat tggccctaaa gtcttccccg caaggccgtg
1201 ggctggacag cgtggtgacg tcgcaacgcg gcgcagggtg agagcgcgcg cttgcggacg
1261 cggcggcatt aaacggttgc aggcgtagag agtggtcgtt gtctttctag gtctcagccg
1321 gtcgtcgcga cgttcgcccg ctcgctctga ggctcctgaa gccgaaacta gctagacttt
1381 cctccttccc gcctgcctgt agcggcgttg ttgccactcc gccaccatgt tcgaggcgcg
1441 cctggtccag ggctccatcc tcaagaaggt gttggaggca ctcaaggacc tcatcaacga
1501 ggcctgctgg gatattagct ccagcggtgt aaacctgcag agcatggact cgtcccacgt
1561 ctctttggtg cagctcaccc tgcggtctga gggcttcgac acctaccgct gcgaccgcaa
1621 cctggccatg ggcgtgaacc tcaccaggtg agcctcgcgc cccgggaagc cgccccggcc
1681 cgcctgcacc tccggctgtg gcgagcgctt cgagcctagc cctcattggc tggcgtgggc
1741 atccagagct tctcattggc ctgcacgcag tggtggggcc caagctgaga tgagcggtta
1801 cggaaaagcc cgcgctggct gctgcgcgaa cctgcttttt cgcgccaaag tcacaaagcg
1861 ggtggtggcg ggaaaatcaa gggtttttcc gcagtgccag gaacactgtt ccagggactc
1921 tttgctcact aaacctgttg gccttgaatg gacgctttag ctgtggcttt cttgtttctg
1981 agacggtctc ggtctcggtg tgttgcccgg gctggtctcc aacttctggg ctcaagcgat
2041 cctcccggct cagtcgcgtc gactttaaat gctttataat gcccttgcga gaaatgtggc
2101 agcctgtcat cctacttagt ggtaggagat tgtttctatc cagaagggac actgctggtg
2161 gtattttagt ataaatactg ccagatgcgt ccaaaacgtc tgcattaata atggcatcct
2221 ccagcagtcc gtttaccctc caccagttct gagacggcct gacgggtgag agtggtaacc
2281 ccttctaacc gcgttcgaaa tacagccctt cagcagacgg cgttgatttt aaagcatgtg
2341 tctcctgtct tctagtatgt ccaaaatact aaaatgcgcc ggcaatgaag atatcattac
2401 actaagggcc gaagataacg cggatacctt ggcgctagta tttgaagcac caagtaagtc
2461 gtaccttttt accgagtcac gaagctacag gaaaatcaaa actctgtgtg agtagaaact
2521 caaaagctat ctgcgtttct tttggtaaga ccaggagaaa gtttcagact atgaaatgaa
2581 gttgatggat ttagatgttg aacaacttgg aattccagtg agtatcagtt tctcattgta
2641 gagagtgctg tacacaggca cgatagttat gtcatagaat gtttgtttat ttttacagac
2701 agggtcttgg ctctgttgcc caggctggag tgcagtagtg ccatatagct ctctctaacc
2761 tgggattcct gggctcaagc agtcctcttg ccttagtctc ctaagtggct aggaaggact
2821 acgggcctgt cccaccacac ctggctaatt tttttcattt ttgtgtgtgg gacgtggggg
2881 cagtctagcc aggctggctg gaactcctgg cctcaagtga tcctcctccg tcaagatatg
2941 ttaatataat ttaaagccta cttcataaca acttttctag aaatatatct actggtgcat
3001 gtttcaaaga gatgatttta gtatttggat agttgttcac cacaagtcta ataatctcca
3061 caggttaaat ttattgttta tgccagttgt ctatttgcat taacttccat gaactcttta
3121 aattgttctc tagaatgctt gctttttatt aatgaggttt taaagctagc ttgagagaaa
3181 tttatccagg ttaggttata aacaccaaag gagagaagaa atgtttgaat gttgaaaatg
3241 cctaatatat tctcttgctt tcttttagaa agtgattagg cctgcttgcg ccatcatgat
3301 ttctgtgcca tactctaatg ttctcttact ttatccctgg aggatgagga ggaggaggct
3361 cttgttccct ggatggtgca tttaatagcc atttattttt ttgagtggag tttgttaaga
3421 aattacgcaa gtcatatttt aaagtaatca gaaaatatga ttctgagttg tttaggtgtt
3481 gccttttaag aaagtgaggg tgccaaatca ttaaatttct aacaattaac ttttggaaaa
3541 ttttgttctt aataggaaca ggagtacagc tgtgtagtaa agatgccttc tggtgaattt
3601 gcacgtatat gccgagatct cagccatatt ggagatgctg ttgtaatttc ctgtgcaaaa
3661 gacggagtga aattttctgc aagtggagaa cttggaaatg gaaacattaa attgtcacag
3721 acaagtaatg tcgataaaga ggaggaagct gtaagtagtt tttaagtaaa aagaaaatag
3781 tttgaagaga attataatac tgcttattag gttaattgct aaaattaaaa gtagacagaa
3841 ttggatccca agtaatttct gaaaattgag atactgttga aatctgtgaa tgatttataa
3901 gtgtcatcca atttagaatt atatttgcaa gaagggaata caaattcagc acgtgtacat
3961 accacagcaa cagtggttta tggatcaagt ccacaccggc tcttaagggt aggattggga
4021 agttaggcgt ataacttagc ttctggagat acttactctc ttaccaaata attgagcata
4081 ggacagcagc tcaatagaag gatatgttag gagtaaaagt ctacctgttt ggagcactta
4141 tgtaatccta atatagctta catgttgtgg gtccaattgg tagcccattt taaaggtgga
4201 gaagcaggct gagcaacctt aagtgacaat ttagccaaag tcacaggctg taggaatcaa
4261 aggttaaaca ggaaggagac tctcactaag gctagaaagc agactccatg caactttgag
4321 agtacctaga gagaccctta tttaaccaaa atagaaagaa catagcaaaa ccccatctct
4381 cataaaaata taaaaattga ccgggtgatg agtggcgaca cacctgtaaa cccgactact
4441 ggaatgcatg agatgggaga atgacttgaa ccgaggaggc ggaggttgca gtgagcccag
4501 atcatgccac tcccctccag cctgggtgac agagcaagat tccatcttaa acaaacaaaa
4561 aaaactcgct aacctgggca taaattaaaa ctttgtaaat caaggacaaa ggtcctaaac
4621 ctcataactt gcattaggat taaatacggt agcattaaag agcttagcat atctgtgtgt
4681 ggcatattat aagcttacaa taaatactat atattgctct cttgtccctt gaatgggtag
4741 tcaacattta gtttaaataa aggtaaaatt cagttgaaag gttttttttt aaattaataa
4801 agtctaggag ctgattcttt atctgtttcc tgaatcacat ttccactcct gccaacctcg
4861 tttttttctt ttgctgtttt tctttgtttt tgagacaggg cttgctctgt gccacccagg
4921 ctggagtgcg gtggtgcagt cggttcacta cagcctcaaa ctccagggct taagtgatcc
4981 tcctgcctca gtttcccaag agccgggaca caggtgtgtg ccaacacact agcctggttt
5041 ccctaatttc attttcccct tgaccattac aactatttgt tgaagaaatt agatcattta
5101 ttagtttcag agtttggatt ttacctgatt gcattcctgt gtatctaata acctctacct
5161 gtgtgtccta cagactggta gctatagcct ggagccttga tatcagggtg ttttgtttcg
5221 ggggtgagag agcaagaata tggtggtggt gtgtgtgcct ctagtaggag gcacagggtg
5281 tctggatgtg tttgcaatgt tagcagctat aagtcattgt ctagatccat taagtcatta
5341 attagagttt gcagagctga aattaatacg ttttatcact tattggctgc ttattagaaa
5401 acttccataa gaaaagcttc ccattatata atttggttat ctaaattata gctataccaa
5461 aagacaaagg ctagataatc gagtcttttt gcatttatgt atcagtcttc aaaattttca
5521 tagcgtccct ccaaagtgac caatacaagt gtttgtgggt ttttataaat atataatgag
5581 ctaatagatt gcaactttct tgatgttttt caatgatgaa tcttttgttt tgtaggttac
5641 catagagatg aatgaaccag ttcaactaac ttttgcactg aggtacctga acttctttac
5701 aaaagccact ccactctctt caacggtgac actcagtatg tctgcagatg taccccttgg
5761 taagataata aatttgaacc ttgttttgat ggtagtcata tgtgatacat actcctcagt
5821 aattaaccat cttcctgtct ttcagttgta gagtataaaa ttgcggatat gggacactta
5881 aaatactact tggctcccaa gatcgaggat gaagaaggat cttaggcatt cttaaaattc
5941 aagaaaataa aactaagctc tttgagaact gcttctaaga tgccagcata tactgaagtc
6001 ttttctgtca ccaaatttgt acctctaagt acatatgtag atattgtttt ctgtaaataa
6061 cctatttttt ttctctattc tctccaattt gtttaaagaa taaagtccaa agtctgatct
6121 ggtctagtta acctagaagt atttttgtct cttagaaata cttgtgattt ttataataca
6181 aaagggtctt gactctaaat gcagttttaa gaattgtttt tgaatttaaa taaagttact
6241 tgaatttcaa agatcacagg gcagtgtctt catttgacca ggactgttga aagtatccta
6301 ctgaattccc agctacagtc accctttgtt caaactgttc
//