LOCUS HUMMYP 2568 bp mRNA linear HUM 07-JAN-1995
DEFINITION Human myeloperoxidase mRNA, complete cds.
ACCESSION J02694
VERSION J02694.1
KEYWORDS glycoprotein; myeloperoxidase; peroxidase.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2568)
AUTHORS Morishita,K., Kubota,N., Asano,S., Kaziro,Y. and Nagata,S.
TITLE Molecular cloning and characterization of cDNA for human
myeloperoxidase
JOURNAL J. Biol. Chem. 262 (8), 3844-3851 (1987)
PUBMED 3029127
COMMENT Original source text: Human (HL-60 cells) cDNA to mRNA, clone
lambda-GM706.
Draft entry and computer-readable sequence of [1] kindly provided
by S.Nagata, 18-FEB-1987.
Although two other ATG codons were found at positions 182-184 and
227-229, the codon at 149-151 was confirmed as the initiation codon
since 'the first ATG codon is usually the initiation codon of
eukaryotic mRNAs' [1].
FEATURES Location/Qualifiers
source 1..2568
/db_xref="H-InvDB:HIT000191081"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="17q21.3-q23"
/cell_line="HL-60"
gene 1..2568
/gene="MPO"
CDS 149..2386
/gene="MPO"
/EC_number="1.11.1.7"
/codon_start=1
/product="myeloperoxidase"
/protein_id="AAA59896.1"
/db_xref="GDB:G00-120-192"
/translation="MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQ
PSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYF
KQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAY
QDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVK
RNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFV
TGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALT
SFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNR
SARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIV
GAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPF
MFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVD
EIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNL
KLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWEN
EGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLAS
WREAS"
mat_peptide 149..982
/gene="MPO"
/product="myeloperoxidase light chain"
/note="G00-120-192"
mat_peptide 983..2383
/gene="MPO"
/product="myeloperoxidase heavy chain"
/note="G00-120-192"
BASE COUNT 513 a 810 c 755 g 490 t
ORIGIN 223 bp upstream of PstI site.
1 gtccttggaa gctggatgac agcagctggc aaggggataa gagagcagtg agcccctccc
61 tcaaggaggt ctggctttat ccatagacag ggccctctga ggtggggctg aggtacaaag
121 ggggattgag cagcccagga gaagagagat gggggttccc ttcttctctt ctctcagatg
181 catggtggac ttaggacctt gctgggctgg gggtctcact gcagagatga agctgcttct
241 ggccctagca gggctcctgg ccattctggc cacgccccag ccctctgaag gtgctgctcc
301 agctgtcctg ggggaggtgg acacctcgtt ggtgctgagc tccatggagg aggccaagca
361 gctggtggac aaggcctaca aggagcggcg ggaaagcatc aagcagcggc ttcgcagcgg
421 ctcagccagc cccatggaac tcctatccta cttcaagcag ccggtggcag ccaccaggac
481 ggcggtgagg gccgctgact acctgcacgt ggctctagac ctgctggaga ggaagctgcg
541 gtccctgtgg cgaaggccat tcaatgtcac tgatgtgctg acgcccgccc agctgaatgt
601 gttgtccaag tcaagcggct gcgcctacca ggacgtgggg gtgacttgcc cggagcagga
661 caaataccgc accatcaccg ggatgtgcaa caacagacgc agccccacgc tgggggcctc
721 caaccgtgcc tttgtgcgct ggctgccggc ggagtatgag gacggcttct ctcttcccta
781 cggctggacg cccggggtca agcgcaacgg cttcccggtg gctctggctc gcgcggtctc
841 caacgagatc gtgcgcttcc ccactgatca gctgactccg gaccaggagc gctcactcat
901 gttcatgcaa tggggccagc tgttggacca cgacctcgac ttcacccctg agccggccgc
961 ccgggcctcc ttcgtcactg gcgtcaactg cgagaccagc tgcgttcagc agccgccctg
1021 cttcccgctc aagatcccgc ccaatgaccc ccgcatcaag aaccaagccg actgcatccc
1081 gttcttccgc tcctgcccgg cttgccccgg gagcaacatc accatccgca accagatcaa
1141 cgcgctcact tccttcgtgg acgccagcat ggtgtacggc agcgaggagc ccctggccag
1201 gaacctgcgc aacatgtcca accagctggg gctgctggcc gtcaaccagc gcttccaaga
1261 caacggccgg gccctgctgc cctttgacaa cctgcacgat gacccctgtc tcctcaccaa
1321 ccgctcagcg cgcatcccct gcttcctggc aggggacacc cgttccagtg agatgcccga
1381 gctcacctcc atgcacaccc tcttacttcg ggagcacaac cggctggcca cagagctcaa
1441 gagcctgaac cctaggtggg atggggagag gctctaccag gaagcccgga agatcgtggg
1501 ggccatggtc cagatcatca cttaccggga ctacctgccc ctggtgctgg ggccaacggc
1561 catgaggaag tacctgccca cgtaccgttc ctacaatgac tcagtggacc cacgcatcgc
1621 caacgtcttc accaatgcct tccgctacgg ccacaccctc atccaaccct tcatgttccg
1681 cctggacaat cggtaccagc ccatggaacc caacccccgt gtccccctca gcagggtctt
1741 ttttgcctcc tggagggtcg tgctggaagg tggcattgac cccatcctcc ggggcctcat
1801 ggccacccct gccaagctga atcgtcagaa ccaaattgca gtggatgaga tccgggagcg
1861 attgtttgag caggtcatga ggattgggct ggacctgcct gctctgaaca tgcagcgcag
1921 cagggaccac ggcctcccag gatacaatgc ctggaggcgc ttctgtgggc tcccgcagcc
1981 tgaaactgtg ggccagctgg gcacggtgct gaggaacctg aaattggcga ggaaactgat
2041 ggagcagtat ggcacgccca acaacatcga catctggatg ggcggcgtgt ccgagcctct
2101 gaagcgcaaa ggccgcgtgg gcccactcct cgcctgcatc atcggtaccc agttcaggaa
2161 gctccgggat ggtgatcggt tttggtggga gaacgagggt gtgttcagca tgcagcagcg
2221 acaggccctg gcccagatct cattgccccg gatcatctgc gacaacacag gcatcaccac
2281 cgtgtctaag aacaacatct tcatgtccaa ctcatatccc cgggactttg tcaactgcag
2341 tacacttcct gcattgaacc tggcttcctg gagggaagcc tcctagaggc caggtaaggg
2401 ggtgcagcag tgaggggtat atctgggctg gccagttgga accacggaga tctccttgcc
2461 ctagatgagc ccagccctgt tctgggtgca gctgagaaaa tgagtgacta gacgttcatt
2521 tgtgtgctca tgtatgtgcg aagtatataa attggctttt catgcgtg
//